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1.
J Biomed Mater Res A ; 111(2): 234-244, 2023 02.
Article in English | MEDLINE | ID: mdl-36239143

ABSTRACT

Microgrooved surfaces are recognized as an important strategy of tissue engineering to promote the alignment of bone cells. In this work, we have investigated the mechanical and morphological aspects of osteoblasts cells after interaction with different micro-structured polymeric surfaces. Femtosecond laser writing technique was used for the construction of circular and parallel microgrooved patterns in biocompatible polymeric surfaces based on pentaerythritol triacrylate. Additionally, we have studied the influence of the biocompatible TiO2 nanocrystals (NCs) related to the cell behavior, when incorporated to the photoresin. The atomic force microscopy technique was used to investigate the biomechanical reaction of the human osteoblast-like MG-63 cells for the different microgroove. It was demonstrated that osteoblasts grown on circular microgrooved surfaces exhibited significantly larger Young's modulus compared to cells sown on flat films. Furthermore, we could observe that TiO2 NCs improved the circular microgrooves effects, resulting in more populated sites, 34% more elongated cells, and increasing the cell stiffness by almost 160%. These results can guide the design and construction of effective scaffold surfaces with circular microgrooves for tissue engineering and bone regeneration.

2.
Acta Parasitol ; 58(3): 334-6, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23990431

ABSTRACT

Serum samples from 169 water buffaloes and 121 beef cattle were analyzed for antibodies to T. gondii by an indirect fluorescent antibody test (IFAT). Positive results were obtained in 27.2% of water buffaloes and 17.4% of cattle. Statistical analysis indicated significant differences between the prevalence in cattle and buffalo (p ≤ 0.05). The highest titres found in positive animals were 1:256 (buffaloes) and 1:64 (cattle). In both bovine species, toxoplasmosis frequency in young animals (less than 2 years old) was lower compared to older individuals, although the differences seen in cattle were not statistically significant.


Subject(s)
Antibodies, Protozoan/blood , Buffaloes , Toxoplasma/immunology , Toxoplasmosis, Animal/epidemiology , Animals , Brazil/epidemiology , Cattle , Fluorescent Antibody Technique, Indirect , Seroepidemiologic Studies , Serum/immunology
3.
Proc Natl Acad Sci U S A ; 109(7): 2585-9, 2012 Feb 14.
Article in English | MEDLINE | ID: mdl-22308470

ABSTRACT

Spreading depression (SD), a slow diffusion-mediated self-sustained wave of depolarization that severely disrupts neuronal function, has been implicated as a cause of cellular injury in a number of central nervous system pathologies, including blind spots in the retina. Here we show that in the hypoglycemic chicken retina, spontaneous episodes of SD can occur, resulting in irreversible punctate lesions in the macula, the region of highest visual acuity in the central region of the retina. These lesions in turn can act as sites of origin for secondary self-sustained reentrant spiral waves of SD that progressively enlarge the lesions. Furthermore, we show that the degeneration of the macula under hypoglycemic conditions can be prevented by blocking reentrant spiral SDs or by blocking caspases. The observation that spontaneous formation of reentrant spiral SD waves leads to the development of progressive retinal lesions under conditions of hypoglycemia establishes a potential role of SD in initiation and progression of macular degeneration, one of the leading causes of visual disability worldwide.


Subject(s)
Hypoglycemia/pathology , Macular Degeneration/pathology , Retina/pathology , Animals , Blotting, Western , Chickens , Immunohistochemistry
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