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1.
Environ Pollut ; 334: 122132, 2023 Oct 01.
Article in English | MEDLINE | ID: mdl-37414124

ABSTRACT

The increased prevalence of human infertility due to male reproductive disorders has been linked to extensive exposure to chemical endocrine disruptors. Acrylamide (AA) is a compound formed spontaneously during the thermal processing of some foods that are mainly consumed by children and adolescents. We previously found that prepubertal exposure to AA causes reduced sperm production and functionality. Oxidative stress is recognized as the main cause of reduced sperm quality and quantity. In this sense, our objective was to evaluate the expression and activity of genes related to enzymatic antioxidant defense, nonprotein thiols, lipid peroxidation (LPO), protein carbonylation (PC) and DNA damage in the testes of rats exposed to acrylamide (2.5 or 5 mg/kg) from weaning to adult life by gavage. For the AA2.5 and AA5 groups, there were no alterations in the transcript expression of genes related to enzymatic antioxidant defense. The enzymatic activities and metabolic parameters were also not affected in the AA2.5 group. For the AA5 group, the enzymatic activities of G6PDH and GPX were reduced, SOD was increased, and protein carbonylation (PC) was increased. Data were also evaluated by Integrate Biomarker Response (IBRv2), a method to analyze and summarize the effects on biomarkers between doses. The IBRv2 index was calculated as 8.9 and 18.71 for AA2.5 and AA5, respectively. The following biomarkers were affected by AA2.5: decreased enzymatic activities of G6PDH, SOD, and GPX, increased GST and GSH, increased LPO and PC, and decreased DNA damage. For AA5, decreased enzymatic activities of G6PDH, GST, CAT and GPX, increased SOD and GSH, increased PC, and decreased LPO and DNA damage were observed. In conclusion, AA exposure during the prepubertal period causes imbalances in the testicular enzymatic antioxidant defense, contributing to the altered spermatic scenario in the testes of these rats.


Subject(s)
Antioxidants , Testis , Humans , Child , Male , Rats , Animals , Adolescent , Antioxidants/metabolism , Protein Carbonylation , Testis/metabolism , Lipid Peroxidation , Acrylamide/toxicity , Acrylamide/metabolism , Semen/metabolism , Oxidative Stress , Superoxide Dismutase/metabolism , Biomarkers/metabolism , Glutathione/metabolism
2.
Arch Toxicol ; 86(4): 663-73, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22120950

ABSTRACT

Sexual differentiation in the brain takes place from late gestation to the early postnatal days. This is dependent on the conversion of circulating testosterone into estradiol by the enzyme aromatase. The glyphosate was shown to alter aromatase activity and decrease serum testosterone concentrations. Thus, the aim of this study was to investigate the effect of gestational maternal glyphosate exposure (50 mg/kg, NOAEL for reproductive toxicity) on the reproductive development of male offspring. Sixty-day-old male rat offspring were evaluated for sexual behavior and partner preference; serum testosterone concentrations, estradiol, FSH and LH; the mRNA and protein content of LH and FSH; sperm production and the morphology of the seminiferous epithelium; and the weight of the testes, epididymis and seminal vesicles. The growth, the weight and age at puberty of the animals were also recorded to evaluate the effect of the treatment. The most important findings were increases in sexual partner preference scores and the latency time to the first mount; testosterone and estradiol serum concentrations; the mRNA expression and protein content in the pituitary gland and the serum concentration of LH; sperm production and reserves; and the height of the germinal epithelium of seminiferous tubules. We also observed an early onset of puberty but no effect on the body growth in these animals. These results suggest that maternal exposure to glyphosate disturbed the masculinization process and promoted behavioral changes and histological and endocrine problems in reproductive parameters. These changes associated with the hypersecretion of androgens increased gonadal activity and sperm production.


Subject(s)
Glycine/analogs & derivatives , Gonadotropins, Pituitary/metabolism , Herbicides/toxicity , Maternal Exposure/adverse effects , Prenatal Exposure Delayed Effects/chemically induced , Reproduction/drug effects , Animals , Brain/drug effects , Brain/metabolism , Estradiol/blood , Female , Gene Expression Regulation, Developmental/drug effects , Glycine/toxicity , Luteinizing Hormone/blood , Male , Mating Preference, Animal/drug effects , Mating Preference, Animal/physiology , Pituitary Gland/drug effects , Pituitary Gland/metabolism , Pregnancy , Prenatal Exposure Delayed Effects/blood , RNA, Messenger/metabolism , Rats , Reproduction/physiology , Seminiferous Epithelium/drug effects , Seminiferous Epithelium/pathology , Sexual Maturation/drug effects , Spermatogenesis/drug effects , Testosterone/blood , Glyphosate
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