ABSTRACT
The influence of diabetes on the enzyme hexokinase (HK) was examined in thesalivary glands of rats. Diabetes was induced by an intraperitoneal injection of streptozotocin(60 mg/Kg body weight) in overnight fasted rats (180-200 g). The animalswere killed 48 hours and 30 days after the induction of diabetes and the submandibularand parotid salivary glands extracted for use. Hyperglycemia was evaluatedby determining the blood sugar. The area occupied by each intralobular component,acini, ducts, total parenchyma and stroma was measured, and no differenceswere observed compared with control. In the soluble fraction of the submandibulargland, no difference in the specific activity of HK was observed, between the diabeticand control animals, however, the activity per gland and per g of tissue showedlower values than control. The specific activity of the bound form was reduced in thediabetic gland. The results obtained for the parotid gland were different from thesubmandibular. The specific activity of both the soluble and bound forms wereincreased in the diabetic animals. The DEAE-cellulose column chromatography ofthe soluble and bound forms of the enzyme from both glands showed a first peakappearing during the washing of the column and two other peaks were eluted by thegradient. Thus, three isoenzymes in the submandibular and parotid salivary glandsfor the control and diabetic rats have been found (AU)
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