ABSTRACT
Cancer can be described as a dynamic disease formed by malignant and stromal cells. The cellular interaction between these components in the tumor microenvironment (TME) dictates the development of the disease and can be mediated by extracellular vesicles secreted by tumor cells (TEVs). In this review, we summarize emerging findings about how TEVs modify important aspects of the disease like continuous tumor growth, induction of angiogenesis and metastasis establishment. We also discuss how these nanostructures can educate the immune infiltrating cells to generate an immunosuppressive environment that favors tumor progression. Furthermore, we offer our perspective on the path TEVs interfere in cancer treatment response and promote tumor recurrence, highlighting the need to understand the underlying mechanisms controlling TEVs secretion and cargo sorting. In addition, we discuss the clinical potential of TEVs as markers of cell state transitions including the acquisition of a treatment-resistant phenotype, and their potential as therapeutic targets for interventions such as the use of extracellular vesicle (EV) inhibitors to block their pro-tumoral activities. Some of the technical challenges for TEVs research and clinical use are also presented.
ABSTRACT
Biodegradable films are a promising strategy to reduce the environmental impact caused by conventional plastics commonly used in agriculture. This study focused on the production and characterization of Konjac glucomannan (KGM) and alginate (ALG) based films enriched with sugarcane vinasse (VIN), a nutrient-rich wastewater generated in large volumes by the sugar-ethanol producing industries. ALG, KGM and ALG/KGM blended (50:50) films were produced by casting and treated with calcium ions (Ca2+) (ALG films) and a combination of Ca2+, alkali, and ethanol (KGM and ALG/KGM films). Vinasse addition tended to reduce transparency and water resistance of the films and had less effect on their mechanical properties. Crosslinking of ALG films resulted in enhanced mechanical properties and reduced moisture content, water solubility, swelling, water vapor permeability, and flexibility. KGM films were less impacted by crosslinking/deacetylation but showed improved water resistance while maintain a high degree of swelling (290% and 185% for KGM and KGM/VIN films respectively). Blended films exhibited characteristic properties of the two biopolymers and adequate compatibility indicated by Fourier transform infrared spectroscopy (FTIR) and morphologies. Vinasse-added ALG/KGM films represent a novel nutrient-enriched, bio-based material for agricultural applications and could help to face the environmental challenges imposed by vinasse disposal.
