Antifungal activities of rotenoids from seeds and roots of Clitoria fairchildiana / Atividade antifúngica dos rotenoides das sementes e raiz de Clitoria fairchildiana

Clitoria fairchildiana (synonym Clitoria racemosa) is a tree belonging to the Leguminosae family growing in several Brazilian regions and it has in its composition rotenoids with unusual structures. The aim of this work is to determinate the antimicrobial activity rotenoids from C. fairchildiana. Clitoriacetal, 6-desoxyclitoriacetal, stemonal and stemonone were isolated from the roots and 11-desoxyclitoriacetal from seeds by different chromatographic techniques and identified by spectrometric data analyzes. The antimicrobial activity was obtained using different culture media and the results confirm the importance of the junction of the ring B/C and the pattern of hydroxylation of these compounds in antifungal activities. This is the first time antimicrobial activities of these rotenoids were determined.(AU)

Clitoria fairchildiana (sinônimo Clitoria racemosa) é uma árvore da família Leguminosa encontrada em várias regiões brasileiras e possui na sua composição rotenoides de estruturas não usuais. O objetivo do presente trabalho é determinar a atividade antimicrobiana de cinco rotenoides isolados das raízes e sementes da C. fairchildiana. Clitoriacetal, 6-desoxiclitoriacetal, stemonal e stemonona foram isolados das raízes e o 11- desoxiclitoriacetal isolado das sementes por meio de diferentes técnicas cromatográficas e identificados através da análise de dados espectrométricos. A atividade antimicrobiana foi obtida utilizando diferentes meios de cultura e os resultados confirmam a importância da junção do anel B/C e o padrão de hidroxilação dos rotenoides na atividade antifúngica. Este é o primeiro relato de atividades antimicrobianas de rotenoides de Clitoria.(AU)

Detection and Quantification of Rotenoids from Clitoria fairchildiana and its Lipids Profile.

This work describes the isolation and quantification of rotenoids from crude organic extracts of different parts of Clitoria fairchildiana R. A. Howard (Leguminosae) by HPLC-DAD. The lipid composition and the Artemia salina cytotoxic activities of the isolates were also conducted. Clitoriacetal (1), 6-deoxyclitoriacetal (2), stemonal and stemonone were isolated by chromatographic procedures and identified by usual spectroscopic and spectrometric techniques. Clitoriacetal and 6-deoxyclitoriacetal were not found in all parts of the plant, such as leaves and petals, but in the roots they occur in higher concentration. The activity against brine shrimp revealed that the root extract (LD50 = 158 ppm) was the more active.

Bioactive oleanane, lupane and ursane triterpene acid derivatives.

Betulinic, ursolic and oleanolic acids isolated from the aerial parts of Eriope blanchetii (Lamiaceae) were subjected to different esterification reactions, yielding 12 C-3 position ester derivatives. All compounds were identified using spectroscopic techniques, such as IR, 1H-NMR and MS. The derivatives were further investigated for their antioxidant level, Artemia salina lethality and antimicrobial activity.