"Como será minha vida com sífilis?" Desafios do diagnóstico em homens à luz de Leininger / "How will my life with syphilis be?" Diagnosis challenges in men in the light of Leininger / ¿Cómo será mi vida con sífilis? Desafíos diagnósticos en los hombres a la luz de Leininger

Objetivo: compreender os fatores que interferem no diagnóstico da sífilis em homens à luz da Teoria da Diversidade e Universalidade do Cuidado Cultural. Método: estudo qualitativo, aprovado por Comitê de Ética em Pesquisa, realizado em Hospital Universitário no Rio de Janeiro, entre setembro de 2017 e março de 2018. Foram entrevistados 32 homens diagnosticados com sífilis adquirida, e a análise temática foi utilizada para o tratamento dos dados. Resultados: a maioria dos homens tinha história de contágio por outra Infecção Sexualmente Transmissível e foi diagnosticada no ambulatório de imunologia. O momento diagnóstico é encarado com surpresa, tem repercussões psicossociais e é influenciado por fatores culturais e sociais. Considerações finais: alguns fatores interferem positivamente e outros negativamente no diagnóstico da sífilis na população masculina. Para detectar essa infecção nos homens, deve-se conhecer o contexto sociocultural em que estão inseridos para, assim, implementar estratégias tanto diagnósticas quanto preventivas mais eficazes(AU)

Objective: to understand the factors that interfere with the diagnosis of syphilis in men in the light of the Theory of Diversity and Universality of Cultural Care. Method: qualitative study, approved by the Ethics and Research Committee, conducted at a University Hospital in Rio de Janeiro, during september 2017 to march 2018. Thirty-two men diagnosed with acquired syphilis were interviewed, and thematic analysis was used for data treatment. Results: most men had a history of contagion by another STI and were diagnosed at the immunology outpatient clinic. The moment of diagnosis is faced with surprise, has psychosocial repercussions and is influenced by cultural and social factors. Final considerations: some factors interfere positively and others negatively in the diagnosis of syphilis in the male population. To detect this infection in men, it is necessary to know the sociocultural context in which they live, in order to implement more effective diagnostic and preventive strategies(AU)

Objetivo: comprender los factores que interfieren en el diagnóstico de la sífilis en los hombres a la luz de la Teoría de la diversidad y universalidad del cuidado cultural. Método: estudio cualitativo, aprobado por el Comité de Ética en Investigación, realizado en un Hospital Universitario de Río de Janeiro, de septiembre de 2017 a marzo de 2018. Se entrevistó a 32 hombres diagnosticados de sífilis adquirida y se utilizó el análisis temático para el tratamiento de los datos. Resultados: la mayoría de los hombres tenía antecedentes de contagio por otra Infección Sexualmente Transmisible y el diagnóstico se hizo en el ambulatorio de inmunología. El momento del diagnóstico se afronta con sorpresa, tiene repercusiones psicosociales y está influenciado por factores culturales y sociales. Consideraciones finales: algunos factores interfieren positivamente y otros negativamente en el diagnóstico de la sífilis en la población masculina. Para detectar esta infección en los hombres, se debe conocer el contexto sociocultural en el que se insertan, para entonces poner en marcha estrategias de diagnóstico y de prevención más eficaces(AU)

Lipidome remodeling in response to nutrient replenishment requires the tRNA modifier Deg1/Pus3 in yeast.

In the yeast Saccharomyces cerevisiae, the absence of the pseudouridine synthase Pus3/Deg1, which modifies tRNA positions 38 and 39, results in increased lipid droplet (LD) content and translational defects. In addition, starvation-like transcriptome alterations and induced protein aggregation were observed. In this study, we show that the deg1 mutant increases specific misreading errors. This could lead to altered expression of the main regulators of neutral lipid synthesis which are the acetyl-CoA carboxylase (Acc1), an enzyme that catalyzes a key step in fatty acid synthesis, and its regulator, the Snf1/AMPK kinase. We demonstrate that upregulation of the neutral lipid content of LD in the deg1 mutant is achieved by a mechanism operating in parallel to the known Snf1/AMPK kinase-dependent phosphoregulation of Acc1. While in wild-type cells removal of the regulatory phosphorylation site (Ser-1157) in Acc1 results in strong upregulation of triacylglycerol (TG), but not steryl esters (SE), the deg1 mutation more specifically upregulates SE levels. In order to elucidate if other lipid species are affected, we compared the lipidomes of wild type and deg1 mutants, revealing multiple altered lipid species. In particular, in the exponential phase of growth, the deg1 mutant shows a reduction in the pool of phospholipids, indicating a compromised capacity to mobilize acyl-CoA from storage lipids. We conclude that Deg1 plays a key role in the coordination of lipid storage and mobilization, which in turn influences lipid homeostasis. The lipidomic effects in the deg1 mutant may be indirect outcomes of the activation of various stress responses resulting from protein aggregation.

Long-term monitoring of COVID-19 prevalence in raw and treated wastewater in Salvador, the largest capital of the Brazilian Northeast.

Wastewater-based epidemiology (WBE) becomes an interesting epidemiological approach to monitoring the prevalence of SARS-CoV-2 broadly and non-invasively. Herein, we employ for the first time WBE, associated or not with the PEG 8000 precipitation method, for the detection of SARS-CoV-2 in samples of raw or treated wastewater from 22 municipal wastewater treatment stations (WWTPs) located in Salvador, the fourth most populous city in Brazil. Our results demonstrate the success of the application of WBE for detecting SARS-CoV-2 in both types of evaluated samples, regardless of the usage of PEG 8000 concentration procedure. Further, an increase in SARS-CoV-2 positivity rate was observed in samples collected in months that presented the highest number of confirmed COVID-19 cases (May/2021, June/2021 and January/2022). While PEG 8000 concentration step was found to significantly increase the positivity rate in treated wastewater samples (p < 0.005), a strong positive correlation (r: 0.84; p < 0.002) between non-concentrated raw wastewater samples with the number of new cases of COVID-19 (April/2021-February/2022) was observed. In general, the present results reinforce the efficiency of WBE approach to monitoring the presence of SARS-CoV-2 in either low- or high-capacity WWTPs. The successful usage of WBE even in raw wastewater samples makes it an interesting low-cost tool for epidemiological surveillance.

Plasma oxylipin profiling by high resolution mass spectrometry reveal signatures of inflammation and hypermetabolism in amyotrophic lateral sclerosis.

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive loss of motor neurons, systemic hypermetabolism, and inflammation. In this context, oxylipins have been investigated as signaling molecules linked to neurodegeneration, although their specific role in ALS remains unclear. Importantly, most methods focused on oxylipin analysis are based on low-resolution mass spectrometry, which usually confers high sensitivity, but not great accuracy for molecular characterization, as provided by high-resolution MS (HRMS). Here, we established an ultra-high performance liquid chromatography HRMS (LC-HRMS) method for simultaneous analysis of 126 oxylipins in plasma. Intra- and inter-day method validation showed high sensitivity (0.3-25 pg), accuracy and precision for more than 90% of quality controls. This method was applied in plasma of ALS rats overexpressing the mutant human Cu/Zn-superoxide dismutase gene (SOD1-G93A) at asymptomatic (ALS 70 days old) and symptomatic stages (ALS 120 days old), and their respective age-matched wild type controls. From the 56 oxylipins identified in plasma, 17 species were significantly altered. Remarkably, most of oxylipins linked to inflammation and oxidative stress derived from arachidonic acid (AA), like prostaglandins and mono-hydroxides, were increased in ALS 120 d rats. In addition, ketones derived from AA and linoleic acid (LA) were increased in both WT 120 d and ALS 120 d groups, supporting that age also modulates oxylipin metabolism in plasma. Interestingly, the LA-derived diols involved in fatty acid uptake and ß-oxidation, 9(10)-DiHOME and 12(13)-DiHOME, were decreased in ALS 120 d rats and showed significant synergic effects between age and disease factors. In summary, we validated a high-throughput LC-HRMS method for oxylipin analysis and provided a comprehensive overview of plasma oxylipins involved in ALS disease progression. Noteworthy, the oxylipins altered in plasma have potential to be investigated as biomarkers for inflammation and hypermetabolism in ALS.

Vulnerabilidade feminina a violência física no período da pandemia de Covid-19 / Women's vulnerability to physical violence during the Covid-19 pandemic / Vulnerabilidad femenina a la violencia física en el período de la pandemia covid-19

Objetivo: analisar os fatores que aumentaram a vulnerabilidade feminina à violência física, no período da quarentena para Covid-19. Método: estudo quantitativo, transversal, descritivo, realizado entre agosto e setembro de 2021. Participaram 154 mulheres. Protocolo aprovado pelo Comitê de Ética em Pesquisa. Resultados: verificou-se que 3,2% tinham ensino fundamental, 80% sofreram violência física, 61,7% tinham ensino superior e 33,7% sofreram violência física. Quanto a renda mensal familiar 6,5% tinham renda menor de R$1.000,00, 80% sofreram violência física, 20,1% tinham renda maior que R$7.000,00, 25,8% sofreram violência física. Quanto ao n.º de filhos, 9,7% tinham mais de 3 filhos, 73,3% sofreram violência física, 18,2% o parceiro fazia uso de drogas ilícitas, 67,9% das mulheres já sofreram violência física. Conclusão: mulheres com baixa escolaridade, baixa renda familiar e maior número de filhos, cujos parceiros (as) fizeram uso de drogas ilícitas apresentaram alta significância estatística e maior vulnerabilidade à violência física durante o período de pandemia para Covid-19.

Objective: to examine factors that increased female vulnerability to physical violence during the quarantine period of the Covid-19 pandemic. Method: this quantitative, cross-sectional, descriptive study was conducted between August and September 2021, with the participation of 154 women. The project was approved by the research ethics committee. Results: of the 3.2% of these women who had only lower secondary schooling, 80% had suffered physical violence; of the 61.7% with higher education, 33.7% had suffered physical violence. In terms of monthly income. Of the 6.5% with monthly income of less than BRL 1,000.00, 80% had suffered physical violence; of the 20.1% earning over BRL 7,000, 25.8% had suffered physical violence. Of the 9.7% with more than 3 children, 73.3% had suffered physical violence; and of the 18.2% whose partners used illegal substances, 67.9% had suffered physical violence. Conclusion: women with little education, low family income, more children, and partners using illegal substances were found, with high statistical significance, to be more vulnerable to physical violence during the Covid-19 pandemic.

Objetivo: analizar los factores que incrementaron la vulnerabilidad femenina a la violencia física durante el período de cuarentena por Covid-19. Método: investigación cuantitativa, transversal, descriptiva, realizada entre agosto y septiembre de 2021. En él participaron 154 mujeres. El protocolo fue aprobado por el Comité de Ética en Investigación. Resultados: se encontró que el 3,2% tenía educación primaria, el 80% había sufrido violencia física, el 61,7% había terminado la universidad y el 33,7% había sufrido violencia física. En cuanto al ingreso familiar mensual, del 6,5% que tenía ingresos inferiores a R$ 1.000,00, el 80% había sufrido violencia física; del 20,1% que tenía ingresos superiores a R$ 7.000,00, el 25,8% había sufrido violencia física. Respecto al número de hijos, el 9,7% tenía más de 3 hijos, el 73,3% sufrió violencia física, la pareja del 18,2% consumía drogas ilícitas, el 67,9% de las mujeres ya había sufrido violencia física. Conclusión: las mujeres con baja escolaridad, bajos ingresos familiares y mayor número de hijos, cuyas parejas consumían drogas ilícitas, presentaron alta significancia estadística y mayor vulnerabilidad a la violencia física durante el período pandémico por Covid-19.

Loss of life estimation and risk level classification due to a dam break.

This report estimated the loss of life and the variation of risk level to people and properties from the city of São José do Jacuípe, at Bahia State, in Brazil, through a simulation of the dam break near the city. The simulations employ the HEC-RAS program with the HEC-GeoRAS plugin, both made available by the U.S. Army Corps of Engineers. The program is a hydrological model for the hydric flow propagation arising from the complete resolution of the Saint-Venant equation, while the plugin was used for vector editing by creating a geomorphological model from the river basin. The results from the research demonstrated that the city is exposed to a risk that is time dependent. In addition, the lack of a warning system about a possible break could cause the death of almost all residents. Otherwise, with a warning system operating, the estimation of destruction would be dramatically reduced.

Bioactive compounds and hepatoprotective effect of Hancornia speciosa gomes fruit juice on acetaminophen-induced hepatotoxicity in vivo.

Hancornia speciosa Gomes (HS) is a Brazilian fruit tree used in inflammatory disorders by folk medicine. Here we sought to investigate the antioxidant and hepatoprotective properties of HS fruit juice in acetaminophen-induced hepatoxicity model in rats, as well as to perform a comprehensive chemical characterization of phenolics and metals by mass spectrometry. HS showed hepatoprotective and antioxidant activity by decreasing MDA and liver injury markers at healthy control levels. In addition, liver histopathological analysis revealed that HS decreases hepatocellular degeneration. Chemical characterization revealed 16 different phenolics, being chlorogenic acid (150 ± 5 µg/g) and rutin (120 ± 8 µg/g) the major phenolics in HS. Among the 14 micronutrients we identified, zinc and boron were the most abundant metals detected in HS. In line with previous studies involving liver diseases, our data supports evidence that such phenolics and metals present in HS may prevent liver injury induced by acetaminophen.

Qualidade de vida das mães de criança com câncer: revisão integrativa / Quality of life of mothers of children with cancer: an integrative review / Calidad de vida de madres de niños con cáncer: una revisión integradora

Objetivo: analisar as evidências científicas da literatura sobre qualidade de vida das mães de criança com câncer. Método: revisão Integrativa da literatura, realizada nas bases de dados LILACS, Medical Subject Headings e CINAHL, nos idiomas inglês, português e espanhol, com utilização dos descritores controlados em português: "qualidade de vida", "câncer", "família" e "enfermagem" e suas versões em inglês e espanhol no recorte temporal de 2010 a 2020, Resultados: foram selecionados 12 estudos completos para análise interpretativa que permitiu a identificação de cinco: categorias: domínios afetados; dados sócios demográficos e qualidade de vida; espiritualidade e pensamento positivo; readaptando o modo de viver; papel da equipe de saúde. Conclusão: os estudos com a temática qualidade de vida das mães de crianças com câncer tiveram a preocupação em mostrar que ter um filho com diagnóstico de câncer altera significativamente a qualidade de vidas das mães, em diferentes aspectos: cognitivo, emocional, afetivo e social.

Objective: to analyze the scientific evidence in the literature on the quality of life of mothers of children with cancer. Method:integrative literature review, carried out in LILACS, Medical Subject Headings (MeSH), CINAHL databases, in English, Portuguese and Spanish, using controlled descriptors in Portuguese: "quality of life", "cancer", "family" and "nursing" and their versions in English and Spanish in the time frame from 2010 to 2020. Results: twelve complete studies were selected for interpretative analysis that allowed the identification of five: categories: affected domains; socio-demographic data and quality of life; spirituality and positive thinking; readapting the way of living; role of the health team. Conclusion: studies with the theme quality of life of mothers of children with cancer were concerned with showing that having a child diagnosed with cancer significantly alters the quality of life of mothers, in different aspects: cognitive, emotional, affective and social.

Objetivo: analizar la evidencia científica em la literatura sobre la calidad de vida de las madres de niños com cáncer. Método: revisión integrativa de la literatura, realizada en LILACS, Medical Subject Headings, bases de datos CINAHL, em inglés, portugués y español, utilizando descriptores controlados em portugués: "calidad de vida", "cáncer", "familia" y "enfermería". Y sus versiones em inglés y español em el período de 2010 a 2020. Resultados: se seleccionaron doce estudios completos para análisis interpretativo que permitieron identificar cinco: categorías: domínios afectados; datos sociodemográficos y calidad de vida; espiritualidad y pensamiento positivo; readaptación de la forma de vida; papel del equipo de salud. Conclusión: estúdios com el tema calidad de vida de madres de niños com cáncer se preocuparon por mostrar que tener um hijo com cáncer altera significativamente la calidad de vida de las madres, en diferentes aspectos: cognitivo, emocional, afectivo y social.

Por que ainda insisto na terapia comunitária? relato de uma experiência formativa e prática / Why do i still insist on community therapy? report of a formative and practical experience / ¿Por qué aún insisto en la terapia comunitaria? informe de experiencia formativa y práctica

A Terapia Comunitária Integrativa valoriza as histórias de vida dos participantes, o resgate da identidade, a restauração da autoestima e da confiança em si e a ampliação da percepção dos problemas e das possibilidades de resolução a partir das competências locais, ou seja, torna o indivíduo mais autônomo e independente. O objetivo deste estudo foi descrever a experiência formativa e a prática da Terapia Comunitária Integrativa. Trata-se de um relato desenvolvido em duas Unidades Primárias de Atenção à Saúde no município de Horizonte, estado do Ceará. Este trabalho teve início a partir da proposta formativa lançada pela Residência Integrada em Saúde da Escola de Saúde Pública do Ceará. A equipe de terapeutas residentes foi composta por três profissionais: uma assistente social, uma cirurgiã-dentista e uma educadora física. As rodas terapêuticas ocorreram em duas unidades de saúde. Um dos desafios que mais persistiu foi a mobilização da comunidade, pois foi observada, durante todo o processo, a variação do público participante, no que diz respeito ao gênero, à idade e ao papel desenvolvido na comunidade. A equipe valorizou o vínculo, tecendo redes de acesso, disponibilizando-se enquanto equipe de terapeutas e demonstrando a importância do cuidado que vai além da clínica. Foi proposta outra forma de cuidado, que valoriza a autonomia do sujeito e sua capacidade, lembrando sempre que há uma identidade na comunidade e que juntos somos mais fortes.

Integrative Community Therapy values the life histories of participants, the recovery of identity, the restoration of self-esteem and self-confidence, and the broadening of perceived problems and possibilities for resolution based on local competences, that is, it creates a more autonomous and independent individual. Thus, this study describes the formative and practical experience of Integrative Community Therapy. An experience report was developed in two Primary Health Care Units at Horizonte, municipality in the state of Ceará, Brazil. This research stemmed from the formative proposal launched by the Integrated Residency in Health, School of Public Health, Ceará. The team of resident therapists consisted of three professionals: a social worker, a dental surgeon, and a physical educator. Therapeutic rounds took place in two Health Units. A persistent challenge concerned mobilizing the community, as the participating public varied throughout the process regarding gender, age, and the role developed in the community. The team valued the bond, weaving access networks, making themselves available as a team of therapists, and showing the importance of care that goes beyond the clinic. They proposed another form of care, one that values the subject's autonomy and ability, without forgetting the community identity and that together we are stronger.

La Terapia Integrativa Comunitaria valora las historias de vida de los participantes, la recuperación de la identidad, la restauración de la autoestima y confianza en sí mismos, la ampliación de la percepción de los problemas y las posibilidades de resolución con base en competencias locales, es decir, hace que el individuo sea más autónomo e independiente. El objetivo de este estudio fue describir la experiencia formativa y práctica de la Terapia Integrativa Comunitaria. Se trata de un informe elaborado en dos Unidades de Atención Primaria de Salud del municipio de Horizonte, en el estado de Ceará (Brasil). Se inició con la propuesta formativa lanzada por la Residencia Integrada de Salud de la Escuela de Salud Pública de Ceará. El equipo de terapeutas residentes estuvo integrado por tres profesionales: una trabajadora social, una dentista y una profesional de educación física. Las rondas terapéuticas se llevaron a cabo en dos Unidades de Salud. Uno de los desafíos que más persistió fue la movilización de la comunidad, ya que se observó a lo largo del proceso la variación del público participante en cuanto al género, la edad y el rol desarrollado en el comunidad. El equipo valoró el vínculo, estableció redes de acceso y se puso a disposición como equipo de terapeutas demostrando la importancia de una atención que va más allá de la clínica. Se propuso otra forma de cuidado, que valora la autonomía y capacidad del sujeto, recordándoles que siempre hay una identidad en la comunidad y que juntos somos más fuertes.

Transtorno relacionado ao uso de substâncias psicoativas: transições na vida de militares em tratamento / Disorder associated with psychoactive substance use: transitions in the lives of military personnel in treatment / Trastorno relacionado con el uso de sustancias psicoactivas: transiciones en la vida de militares en tratamento

Objetivo: delinear a terapêutica de enfermagem para militares internados em tratamento para transtorno relacionado ao uso de substâncias psicoativas à luz da Teoria das Transições. Método: estudo qualitativo, realizado em 2019, com 11 militares internados em uma unidade psiquiátrica, que responderam a uma entrevista aberta utilizando a técnica Narrativa de Vida. Foram tratados com emprego da técnica de análise de conteúdo. A pesquisa foi submetida e aprovada pelo Comitê de Ética. Resultados: Foram identificados três tipos de transição: situacional, organizacional e saúde-doença. Quanto ao padrão, as transições foram sequenciais, simultâneas e relacionadas. Como indicador de processo se destacou a formação de uma rede de apoio incluindo a família e os profissionais de saúde. Conclusão: a terapêutica de enfermagem envolve a conscientização do militar quanto ao processo de transição, sendo importante a participação da família, a preparação para a transição e a suplementação de papéis durante a internação.

Objective: in the light of Transitions Theory, to outline nursing therapy for inpatient military personnel in treatment for disorders due to psychoactive substance use. Method: this qualitative study was conducted in 2019 with 11 military personnel hospitalized in a psychiatric unit, who responded to an open interview using the Narrative of Life technique. The data were treated using the content analysis technique. The study was submitted to, and approved by, the research ethics committee. Results: three types of transition were identified: situational, organizational and health-disease. In pattern, the transitions were sequential, simultaneous, and related. The salient process indicator was the creation of a support network including the family and health professionals. Conclusion: the nursing therapy involved making the serviceman aware of the transition process, in which the family's participation was important, as was preparation for the transition and role supplementation during hospitalization.

Objetivo: definir la terapia de enfermería para militares hospitalizados en tratamiento por trastornos relacionados con el uso de sustancias psicoactivas a la luz de la Teoría de la Transición. Método: estudio cualitativo, realizado en 2019, junto a 11 militares hospitalizados en una unidad psiquiátrica, quienes respondieron a una entrevista abierta utilizando la técnica Narrativa de Vida. Fueron tratados mediante la técnica de análisis de contenido. La investigación fue presentada y aprobada por el Comité de Ética. Resultados: Se identificaron tres tipos de transición: situacional, organizacional y salud-enfermedad. En cuanto al patrón, las transiciones fueron secuenciales, simultáneas y relacionadas. Como indicador de proceso, resaltó la conformación de una red de apoyo, incluyendo a la familia y los profesionales de la salud. Conclusión: la terapia de enfermería involucra la concientización militar en cuanto al proceso de transición, siendo importante la participación de la familia, la preparación para la transición y la suplementación de roles durante la hospitalización.

Immune status, well-being and gut microbiota in military supplemented with synbiotic ice cream and submitted to field training: a randomised clinical trial.

Strenuous physical activity, sleep deprivation and psychological stress are common features of military field training. The present study aimed to evaluate the effects of supplementation with a synbiotic ice cream on salivary IgA, gastrointestinal symptoms, well-being indicators and gut microbiota in young military participants undergoing field training. Sixty-five military completed the study: one group was supplemented for 30 d with synbiotic ice cream containing: 2·1 × 108 CFU/g for Lactobacillus acidophilus LA-5 and 2·7 × 109 CFU/g for Bifidobacterium animalis BB-12 and 2·3 g of inulin in the 60 g of ice cream at manufacture, and the other with a placebo ice cream. Volunteers were evaluated at pre-supplementation (baseline), post-supplementation and after a 5-d military training. Bifidobacterium and Lactobacillus genera were measured in stool samples and both showed a higher differential abundance post-supplementation and training. Salivary IgA and gastrointestinal symptoms decreased at post-training in both groups (P < 0·05; main effect of time); however, supplementation with synbiotic did not mitigate this effect. Tenseness and sleepiness were decreased in the synbiotic-treated group, but not in the placebo group at post-military training (P = 0·01 and 0·009, respectively; group × time effect). The other well-being indicators were not affected by the synbiotic supplementation. In conclusion, 30 d of synbiotic ice cream supplementation containing inulin, L. acidophilus LA-5 and B. animalis BB-12 favourably modulated gut microbiota and improved tenseness and sleepiness in healthy young military undergoing a 5-d field training. These improvements may be relevant to this population as they may influence the decision-making process in an environment of high physical and psychological stress.

Transition of blind women to motherhood from the perspective of Transitions Theory.

OBJECTIVE: to analyze the transition of blind women to motherhood from the perspective of Transitions Theory. METHOD: a qualitative, descriptive study, which had as participants 11 blind women. An open interview was conducted using the narrative method. Analysis occurred in the light of Transitions Theory, with approval from the Research Ethics Committee with Human Beings. RESULTS: the age group was 32 to 63 years, mostly Catholic, with social security benefits. Transition to motherhood mainly evidenced the experience when becoming a mother and feelings related to this new phase of life. FINAL CONSIDERATIONS: the women in the study adapted themselves to the maternal role, even with difficulties, developed healthy relationships with their children, overcame their disability and nurtured dreams and desires. They were aware of their role, achieving with mastery a healthy transition. It was evidenced scarcity of nursing therapies.

Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture.

The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitro antioxidant activities by DPPH, TBARS, NO assays and cell viability assays. The ultra-high performance liquid chromatography followed by tandem mass spectrometry analysis showed the presence of rutin and caffeic acid in aqueous and ethanolic extract. The total polyphenols in aqueous and ethanolic extracts found were 142.7 ± 3.0 and 123.1 ± 5.8 µg/mL of GAE, respectively. The ethanolic extract (5 mg/mL) inhibits TBARS by 33.8%, and the aqueous extract (5 mg/mL) exhibited scavenger property against nitric oxide derivatives to an extent of 77.8%. In cell culture, both extracts improved cell survivability under H2O2 induced oxidative stress. Thus, C. erecta extract is a good candidate to become a phytotherapic medicine.

Research in nursing and modification of the knowledge tree in CNPq: contribution to science.

OBJECTIVE: to identify funding demands in the light of CNPq's knowledge subareas, as well as data on researchers and research groups, their distribution in the regions and their approximation with health research priorities of the Ministry of Health and the new knowledge tree framework of the area. METHOD: a descriptive study developed with data from 2009 to 2014 about researchers, funding and research areas provided by the Diretoria de Ciências Agrárias Biológicas e Saúde (Department of Agriculture, Biology and Health Sciences). RESULTS: there is a concentration of researchers and research groups in the Southeast region. The research priorities in the health field most frequently addressed were: Noncommunicable Diseases, Child and Adolescent Health, Women's Health and Communicable diseases. The grants could be classified in the current framework of knowledge areas. FINAL CONSIDERATIONS: this analysis enabled visualizing subarea demands and relevance of changing the nomenclature of the classification of knowledge framework in order to represent the scientific production generated in the nursing area.

Malformation and death X Alcoholism: perspective of Nursing the Theory of Transitions for alcoholic pregnant women.

OBJECTIVE: To analyze the knowledge of women who are being treated in Psychosocial Care Centers for Alcohol and Drugs about the harm caused by alcohol consumption during pregnancy, especially regarding fetal malformation. METHOD: Qualitative, descriptive and exploratory study using the Life Narrative approach. Data was collected between February and May 2016, in the city of Rio de Janeiro, with an open interview with the guiding question: "Tell me about your life in relation to alcohol consumption during pregnancy and the guidance received in prenatal care". RESULTS: The narratives revealed lack of information and fear of malformation (physical) and fetal death due to alcohol consumption during pregnancy. DISCUSSION: Malformation or fetal death may trigger a transitional process in women. FINAL CONSIDERATIONS: Women have incipient knowledge about the effects of alcohol on the fetus. They consider that alcohol can only cause physical defects in the children.

Research in nursing and modification of the knowledge tree in CNPq: contribution to science / Investigación en enfermería y modificación del árbol del conocimiento en CNPq: contribución a la ciencia / Pesquisa em enfermagem e a modificação da árvore do conhecimento no CNPq: contribuição à ciência

ABSTRACT Objective: to identify funding demands in the light of CNPq's knowledge subareas, as well as data on researchers and research groups, their distribution in the regions and their approximation with health research priorities of the Ministry of Health and the new knowledge tree framework of the area. Method: a descriptive study developed with data from 2009 to 2014 about researchers, funding and research areas provided by the Diretoria de Ciências Agrárias Biológicas e Saúde (Department of Agriculture, Biology and Health Sciences). Results: there is a concentration of researchers and research groups in the Southeast region. The research priorities in the health field most frequently addressed were: Noncommunicable Diseases, Child and Adolescent Health, Women's Health and Communicable diseases. The grants could be classified in the current framework of knowledge areas. Final considerations: this analysis enabled visualizing subarea demands and relevance of changing the nomenclature of the classification of knowledge framework in order to represent the scientific production generated in the nursing area.

RESUMEN Objetivo: identificar demandas de financiamiento de las subáreas de conocimiento del CNPq, datos sobre investigadores, grupos de investigación, su distribución en las regiones de Brasil y su aproximación con prioridades de investigación en salud del Ministerio de Salud y de la nueva estrutura del árbol de conocimiento del área. Método: estudio descriptivo desarrollado con datos entre 2009 a 2014 sobre investigadores, financiamiento y áreas de investigación suministrados por la Diretoria de Ciências Agrárias Biológicas e Saúde (Dirección de Ciencias Agrarias Biológicas y Salud). Resultados: hay concentración de investigadores y grupos de investigación en la región Sudeste. Las áreas de la agenda de prioridades de investigación en salud más contempladas fueron: Enfermedades no Transmisibles, Salud del Niño y del Adolescente, Salud de la Mujer y Enfermedades Transmisibles. Las concesiones realizadas pudieron ser clasificadas en el actual árbol del conocimiento. Consideraciones finales: el análisis permite la visualización de las demandas de las subáreas y la pertinencia de la modificación de la nomenclatura del árbol del conocimiento, reflejando la producción generada por el área de enfermería.

RESUMO Objetivo: identificar demandas de financiamento das subáreas de conhecimento do CNPq, dados sobre pesquisadores, grupos de pesquisa, sua distribuição nas regiões do Brasil e sua aproximação com prioridades de pesquisa em saúde do Ministério da Saúde e do novo arcabouço da árvore de conhecimento da área. Método: estudo descritivo desenvolvido com dados entre 2009 e 2014, sobre pesquisadores, financiamento e áreas de pesquisa, fornecidos pela Diretoria de Ciências Agrárias Biológicas e Saúde. Resultados: há concentração de pesquisadores e grupos de pesquisa no Sudeste. As áreas da Agenda Nacional de Prioridades de Pesquisa em Saúde mais contempladas foram: Doenças Não Transmissíveis, Saúde da Criança e do Adolescente, Saúde da Mulher e Doenças Transmissíveis. As concessões realizadas puderam ser classificadas na atual árvore do conhecimento. Considerações finais: A análise permite a visualização das demandas das subáreas e pertinência da modificação da nomenclatura da árvore do conhecimento, refletindo a produção gerada pela área.

Malformation and death X Alcoholism: perspective of Nursing the Theory of Transitions for alcoholic pregnant women / Malformación y muerte X Alcoholismo: Perspectiva de Enfermería con la Teoría De La Transición en embarazada / Malformação e morte X Alcoolismo: perspectiva da Enfermagem com a Teoria da Transição em gestantes

ABSTRACT Objective: To analyze the knowledge of women who are being treated in Psychosocial Care Centers for Alcohol and Drugs about the harm caused by alcohol consumption during pregnancy, especially regarding fetal malformation. Method: Qualitative, descriptive and exploratory study using the Life Narrative approach. Data was collected between February and May 2016, in the city of Rio de Janeiro, with an open interview with the guiding question: "Tell me about your life in relation to alcohol consumption during pregnancy and the guidance received in prenatal care". Results: The narratives revealed lack of information and fear of malformation (physical) and fetal death due to alcohol consumption during pregnancy. Discussion: Malformation or fetal death may trigger a transitional process in women. Final Considerations: Women have incipient knowledge about the effects of alcohol on the fetus. They consider that alcohol can only cause physical defects in the children.

RESUMEN Objetivo: Analizar el conocimiento que poseen las mujeres tratadas por alcohol y drogas en los Centros de Atención Sicosocial, sobre los perjuicios que éstos ocasionan en la gestación. Método: investigación cualitativa, descriptiva y exploratoria en la que se utilizó el método Narrativa de Vida, con datos recolectados entre febrero y mayo de 2016 en el municipio de Rio de Janeiro mediante entrevista abierta e interpelaciones orientadoras: "Hábleme al respecto del uso de bebida alcohólica durante la gestación y sobre las orientaciones recibidas en el prenatal". Resultados: falta de información y miedo de malformación (física) y muerte fetal. Discusión: La malformación y/o la muerte fetal pueden desencadenar un proceso transicional en las mujeres. Consideraciones Finales: Las mujeres tienen un conocimiento precario sobre los efectos del alcohol en el feto. Consideran que la bebida influencia más que nada en la aparición de defectos físicos en sus hijos.

RESUMO Objetivo: Analisar o conhecimento das mulheres, tratadas nos Centros de Atenção Psicossocial para álcool e drogas, acerca dos malefícios ocasionados pelo álcool na gestação, principalmente em relação à malformação fetal. Método: Pesquisa qualitativa, descritiva e exploratória. Utilizou o método Narrativa de Vida. Dados coletados entre fevereiro e maio de 2016, no município do Rio de Janeiro, com entrevista aberta e questão norteadora: "Fale-me a respeito de sua vida que tenha relação com uso de bebida alcoólica durante a gestação e as orientações recebidas no pré-natal". Resultados: As narrativas revelaram falta de informação e medo de malformação (física) e morte fetal relacionados ao uso do álcool por gestantes. Discussão: Malformação ou morte fetal podem desencadear um processo transicional nas mulheres. Considerações Finais: As mulheres têm incipiente conhecimento de que o álcool pode acarretar danos ao feto. Consideram que a bebida pode influenciar, somente, no aparecimento de defeitos físicos nos filhos.

Transition of blind women to motherhood from the perspective of Transitions Theory / Transición de mujeres ciegas para la maternidad en la perspectiva de la Teoría de las Transiciones / Transição de mulheres cegas para a maternidade na perspectiva da Teoria das Transições

ABSTRACT Objective: to analyze the transition of blind women to motherhood from the perspective of Transitions Theory. Method: a qualitative, descriptive study, which had as participants 11 blind women. An open interview was conducted using the narrative method. Analysis occurred in the light of Transitions Theory, with approval from the Research Ethics Committee with Human Beings. Results: the age group was 32 to 63 years, mostly Catholic, with social security benefits. Transition to motherhood mainly evidenced the experience when becoming a mother and feelings related to this new phase of life. Final considerations: the women in the study adapted themselves to the maternal role, even with difficulties, developed healthy relationships with their children, overcame their disability and nurtured dreams and desires. They were aware of their role, achieving with mastery a healthy transition. It was evidenced scarcity of nursing therapies.

RESUMEN Objetivo: analizar la transición de mujeres ciegas para la maternidad en la perspectiva de la Teoría de las Transiciones. Método: investigación cualitativa, descriptiva, con 11 mujeres ciegas participando. Se realizó una entrevista abierta utilizando el método Narrativa de la vida y el análisis se realizó a la luz de la Teoría de la transición, con la aprobación del Comité de ética en investigación con seres humanos. Resultados: el grupo de edad tenía entre 32 y 63 años, la mayoría católicos con beneficios de seguridad social. La transición de la maternidad mostró principalmente la experiencia de convertirse en madre y los sentimientos relacionados con esta nueva fase de la vida. Consideraciones finales: las mujeres que participaron en el estudio se adaptaron al papel materno, a pesar de las dificultades, desarrollaron relaciones saludables con sus hijos, superaron la discapacidad y cultivaron sueños y deseos, conscientes de su papel, logrando el dominio de la transición saludable. Había escasez de terapias de enfermería.

RESUMO Objetivo: analisar a transição de mulheres cegas para a maternidade na perspectiva da Teoria das Transições. Método: pesquisa qualitativa, descritiva, que teve como participantes 11 mulheres cegas. Realizou-se entrevista aberta por meio do método de Narrativa de Vida, e a análise ocorreu à luz da Teoria das Transições, com aprovação do Comitê de Ética em Pesquisa com Seres Humanos. Resultados: a faixa etária foi de 32 a 63 anos, a maioria era católica e com benefícios da seguridade social. A transição da maternidade evidenciou principalmente a experiência vivenciada ao se tornar mãe e sentimentos relacionados a essa nova fase da vida. Considerações finais: as mulheres participantes do estudo se adaptaram ao papel materno, mesmo com as dificuldades, desenvolveram relações saudáveis com os filhos, superaram a deficiência e cultivaram sonhos e desejos, conscientes de seu papel, alcançando a maestria da transição saudável. Evidenciou-se a escassez das terapêuticas de enfermagem.

Transition of blind women to motherhood from the perspective of Transitions Theory / Transición de mujeres ciegas para la maternidad en la perspectiva de la Teoría de las Transiciones / Transição de mulheres cegas para a maternidade na perspectiva da Teoria das Transições

ABSTRACT Objective: to analyze the transition of blind women to motherhood from the perspective of Transitions Theory. Method: a qualitative, descriptive study, which had as participants 11 blind women. An open interview was conducted using the narrative method. Analysis occurred in the light of Transitions Theory, with approval from the Research Ethics Committee with Human Beings. Results: the age group was 32 to 63 years, mostly Catholic, with social security benefits. Transition to motherhood mainly evidenced the experience when becoming a mother and feelings related to this new phase of life. Final considerations: the women in the study adapted themselves to the maternal role, even with difficulties, developed healthy relationships with their children, overcame their disability and nurtured dreams and desires. They were aware of their role, achieving with mastery a healthy transition. It was evidenced scarcity of nursing therapies.

RESUMEN Objetivo: analizar la transición de mujeres ciegas para la maternidad en la perspectiva de la Teoría de las Transiciones. Método: investigación cualitativa, descriptiva, con 11 mujeres ciegas participando. Se realizó una entrevista abierta utilizando el método Narrativa de la vida y el análisis se realizó a la luz de la Teoría de la transición, con la aprobación del Comité de ética en investigación con seres humanos. Resultados: el grupo de edad tenía entre 32 y 63 años, la mayoría católicos con beneficios de seguridad social. La transición de la maternidad mostró principalmente la experiencia de convertirse en madre y los sentimientos relacionados con esta nueva fase de la vida. Consideraciones finales: las mujeres que participaron en el estudio se adaptaron al papel materno, a pesar de las dificultades, desarrollaron relaciones saludables con sus hijos, superaron la discapacidad y cultivaron sueños y deseos, conscientes de su papel, logrando el dominio de la transición saludable. Había escasez de terapias de enfermería.

RESUMO Objetivo: analisar a transição de mulheres cegas para a maternidade na perspectiva da Teoria das Transições. Método: pesquisa qualitativa, descritiva, que teve como participantes 11 mulheres cegas. Realizou-se entrevista aberta por meio do método de Narrativa de Vida, e a análise ocorreu à luz da Teoria das Transições, com aprovação do Comitê de Ética em Pesquisa com Seres Humanos. Resultados: a faixa etária foi de 32 a 63 anos, a maioria era católica e com benefícios da seguridade social. A transição da maternidade evidenciou principalmente a experiência vivenciada ao se tornar mãe e sentimentos relacionados a essa nova fase da vida. Considerações finais: as mulheres participantes do estudo se adaptaram ao papel materno, mesmo com as dificuldades, desenvolveram relações saudáveis com os filhos, superaram a deficiência e cultivaram sonhos e desejos, conscientes de seu papel, alcançando a maestria da transição saudável. Evidenciou-se a escassez das terapêuticas de enfermagem.

Does antennal sensilla pattern of different populations of Triatoma maculata (Hemiptera: Reduviidae) reveal phenotypic variability?

BACKGROUND: In Brazil, Triatoma maculata is only found in the State of Roraima and is a vector of Trypanosoma cruzi, the etiological agent of Chagas disease. It occurs in wild, peridomestic and domestic habitats, with an urban infestation in Boa Vista, the capital of this Brazilian state. The aim of this study was to assess the morphological variability of the T. maculata antennal phenotype in three populations of Roraima State, using the antennal sensilla pattern analyzed under optical microscopy. METHODS: The number and distribution of four antennal sensilla types (bristles, thin and thick walled trichoidea, and basiconic) of three Brazilian populations of T. maculata from Roraima State were compared. Univariate and multivariate analyses were performed. RESULTS: The antenna of T. maculata presented the four types of sensilla. According to the density and distribution of the antennal sensilla characteristics, the multivariate analyses showed that the laboratory population is morphologically structured. Urban specimens showed a pronounced phenotypic variability. The main differences were observed in the pedicel segment, and between males and females. CONCLUSIONS: We determined the antennal phenotype in three Roraima populations of T. maculata. These results support the idea that the patterns of antennal sensilla are sensitive markers for distinct populations in the Triatominae. The infestations of T. maculata in different habitats reinforces the ability of this vector to become adapted to a variety of environments, which, could have eco-epidemiological implications for the T. cruzi transmission that are still not well understood.