Blood parameters and hepatic histopathology of broilers fed rations supplemented with essential oils

This study aimed to evaluate the hematological profile, hepatic function, and histopathology of mixed-sex broilers fed rations supplemented with microencapsulated essential oils from Cymbopogon flexuosus (lemon grass) and Lippia rotundifolia (chá-de-pedestre). One hundred and fifty Cobb chicks were housed in cages from 1 to 42 days of age in a completely randomized design, with six replicates with five chickens in each of five treatments (150 total chicks): basal diet without antibiotic growth promoter (negative control), diet with enramicina and salinomycin (positive control), diet with lemon grass essential oil, diet with L. rotundifolia essential oil, and diet with a mixture of lemon grass and L. rotundifolia essential oils. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were significantly lower in untreated broilers (negative control group). Gender was not associated with erythrogram values, but aspartate aminotransferase activity (AST) was higher in females. Gamma-glutamyl transferase (GGT) was higher in chicks in the group that received rations with lemon grass oil. Broilers that received L. rotundifolia oil developed more hepatic lesions, although no effect of sex was observed related to the lesion score. Biliary hyperplasia and fibroplasias were observed in all groups, with higher histopathology scores in broilers that received diets containing L. rotundifolia oil. Mixed-sex broilers fed rations supplemented with lemon grass and L. rotundifolia essential oils have normal complete blood counts and unspecific hepatic lesions and are characterized by lipidosis, hyperplasia of the bile ducts, and fibroplasia.(AU)

Antibacterial activity and stability of microencapsulated lemon grass essential oil in feeds for broiler chickens / Atividade antimicrobiana e estabilidade do óleo essencial de capim-limão (Cymbopogon citratus) microencapsulados em ração para frangos de corte

The antibacterial effect of microencapsulated lemon grass (Cymbopogon citratus) essential oil on strains of Escherichia coli (ATCC8739), Staphylococcus aureus (ATCC 6538) and Salmonella enterica subsp. enterica (ATCC 6017), and the stability of this oil in feeds for broiler chickens were evaluated. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the macrodilution method, using the microencapsulated lemon grass essential oil at concentrations of 160 μL mL−1, 80 μL mL−1, 40 μL mL−1, 20 μL mL−1, and 10 μL mL−1. The oil concentration of 80 μL mL−1 presented the best results against the three bacteria evaluated. Samples of 200 g of feed mixed with 120 μL g−1 of the microencapsulated lemon grass essential oil was stored to evaluate the oil stability. Feed without microencapsulated lemon grass essential oil was prepared as control. The oil remained active for seven days, with significant reduction of S. aureus (3.08 CFU), E. coli (3.01 CFU), and S. enterica (3.10 CFU). The microencapsulated lemon grass essential oil at concentration of 80 μL mL−1 had antibacterial effect against the E. coli, S. enterica and S. aureus, and maintained stability of the feed for seven days, even with presence of organic matter, which is source of nutrients for pathogens.(AU)

No trabalho foi avaliado o efeito antimicrobiano do óleo capim limão (Cymbopogon citratus) microencapsulado frente a cepas de Escherichia coli (ATCC8739), Staphylococcus aureus (ATCC 6538) e Salmonella enterica subsp. enterica (ATCC 6017), e a estabilidade do óleo em ração de frangos de corte. O teste de Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM) do óleo essencial de capim-limão microencapsulado foi determinado pelo método de macrodiluição (tubo) utilizando as concentrações de 160μL/mL, 80μL/mL, 40μL/mL, 20μL/mLe 10μL/mL. A concentração do óleo de 80μL/mL apresentou melhores resultados frente às três bactérias. Para a avaliação da estabilidade foram separados e armazenadas porções de 200g de ração misturadas com o óleo essencial de capim limão na dosagem de 120μL/g de ração, foram preparadas porções de ração sem a presença do óleo essencial para teste controle, os resultados mostraram que o óleo permaneceu ativo até sete dias, com redução significativa de 3,08 UFC para S. aureus, 3, 01 para E. coli e 3,10 S. entérica. Conclui-se que, óleo essencial de capimlimão microencapuslado tem atividade antibacteriana na concentração de 80 μL/mL frente à Escherichia coli, S. entérica e S. aureus e promove a estabilidade da ração no período de sete dias mesmo na presença da matéria orgânica que é fonte de nutrientes para patógenos.(AU)

Antibacterial activity and stability of microencapsulated lemon grass essential oil in feeds for broiler chickens / Atividade antimicrobiana e estabilidade do óleo essencial de capim-limão (Cymbopogon citratus) microencapsulados em ração para frangos de corte

The antibacterial effect of microencapsulated lemon grass (Cymbopogon citratus) essential oil on strains of Escherichia coli (ATCC8739), Staphylococcus aureus (ATCC 6538) and Salmonella enterica subsp. enterica (ATCC 6017), and the stability of this oil in feeds for broiler chickens were evaluated. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the macrodilution method, using the microencapsulated lemon grass essential oil at concentrations of 160 μL mL−1, 80 μL mL−1, 40 μL mL−1, 20 μL mL−1, and 10 μL mL−1. The oil concentration of 80 μL mL−1 presented the best results against the three bacteria evaluated. Samples of 200 g of feed mixed with 120 μL g−1 of the microencapsulated lemon grass essential oil was stored to evaluate the oil stability. Feed without microencapsulated lemon grass essential oil was prepared as control. The oil remained active for seven days, with significant reduction of S. aureus (3.08 CFU), E. coli (3.01 CFU), and S. enterica (3.10 CFU). The microencapsulated lemon grass essential oil at concentration of 80 μL mL−1 had antibacterial effect against the E. coli, S. enterica and S. aureus, and maintained stability of the feed for seven days, even with presence of organic matter, which is source of nutrients for pathogens.

No trabalho foi avaliado o efeito antimicrobiano do óleo capim limão (Cymbopogon citratus) microencapsulado frente a cepas de Escherichia coli (ATCC8739), Staphylococcus aureus (ATCC 6538) e Salmonella enterica subsp. enterica (ATCC 6017), e a estabilidade do óleo em ração de frangos de corte. O teste de Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM) do óleo essencial de capim-limão microencapsulado foi determinado pelo método de macrodiluição (tubo) utilizando as concentrações de 160μL/mL, 80μL/mL, 40μL/mL, 20μL/mLe 10μL/mL. A concentração do óleo de 80μL/mL apresentou melhores resultados frente às três bactérias. Para a avaliação da estabilidade foram separados e armazenadas porções de 200g de ração misturadas com o óleo essencial de capim limão na dosagem de 120μL/g de ração, foram preparadas porções de ração sem a presença do óleo essencial para teste controle, os resultados mostraram que o óleo permaneceu ativo até sete dias, com redução significativa de 3,08 UFC para S. aureus, 3, 01 para E. coli e 3,10 S. entérica. Conclui-se que, óleo essencial de capimlimão microencapuslado tem atividade antibacteriana na concentração de 80 μL/mL frente à Escherichia coli, S. entérica e S. aureus e promove a estabilidade da ração no período de sete dias mesmo na presença da matéria orgânica que é fonte de nutrientes para patógenos.