Effect of elite physical exercise by triathletes on seven catabolites of DNA oxidation.

The oxidized nucleoside 8-hydroxy-2'-deoxyguanosine has been widely studied as a marker of DNA oxidation; however, data on the occurrence of other metabolites in plasma that are related to DNA damage are scarce. We have applied an improved, sensitive, robust, and reliable method, involving solid phase extraction and ultrahigh-performance liquid chromatography (UHPLC)-tandem mass spectrometry (MS/MS), to the precise quantitation of seven metabolites in the plasma of 15 elite triathletes after a 2-week training program. All compounds were eluted in the first 1.6 min, with limits of detection and quantification ranging between 0.001 and 0.3 ng.mL(-1) and 0.009 and 0.6 ng.mL(-1), respectively. Four compounds were detected in plasma: guanosine-3'-5'-cyclic monophosphate, 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanosine, and 8-nitroguanosine. After two weeks of training, 8-hydroxyguanine exhibited the highest increase (from 0.031 ± 0.008 nM to 0.036 ± 0.012 nM) (p < 0.05), which could be related to the enhanced activity of DNA-repairing enzymes that excise this oxidized base. Increased levels of guanosine-3'-5'-cyclic monophosphate and 8-hydroxy-2'-deoxyguanosine were also observed. In contrast, levels of 8-nitroguanosine (p < 0.05) were significantly reduced, which might be a protective measure as this compound strongly stimulates the generation of superoxide radicals, and its excess is related to pathologies such as microbial (viral) infections and other inflammatory and degenerative disorders. The results obtained indicate an induced adaptive response to the increased oxidative stress related to elite training, and point to the benefits associated with regular exercise.

Influence of pollutants in the magneto-dielectric response of silicon nanoparticles.

The influence of the degree of purity of a silicon nanoparticle on its resonances, either electric or magnetic, is assessed by using Mie theory as well as finite-element simulations. In particular, it is shown that the main effect of the increase of absorption due to the pollutants is observed in the magnetic resonances. Concerning Kerker's conditions for the directionality of the scattering [J. Opt. Soc. Am.73, 765 (1983)], it is found that both are strongly shifted when the material's purity is varied. Resistive losses confirm the quenching of magnetic resonances, showing that the region of influence in the magnetic dipole resonance is much larger than in the electric one, although it has been found that losses are not critical for silicon content over 99.50%.

Comprehensive polarimetric analysis of Spectralon white reflectance standard in a wide visible range.

Since polarimetry has extended its use for the study of scattering from surfaces and tissues, Spectralon, a white reflectance standard, is acquiring the role of a polarimetric standard. Both the behavior of Spectralon as a Lambertian surface and its performance as a perfect depolarizer are analyzed in detail. The accuracy of our dynamic polarimeter, together with the polar decomposition to describe the Mueller matrix (MM) depolarizing action, combine to produce a powerful tool for the proper analysis of this scattering surface. Results allowed us to revisit, for confirmation or revision, the role of some MM elements, as described in the bibliography. The conditions under which it can be considered a good Lambertian surface are specified in terms of incidence and scattering angle and verified over a large wavelength range.

Non-targeted metabolomic approach reveals urinary metabolites linked to steroid biosynthesis pathway after ingestion of citrus juice.

Citrus juice intake has been highlighted because of its health-promoting effects. LC-MS based metabolomics approaches are applied to obtain a better knowledge on changes in the concentration of metabolites due to its dietary intake and allow a better understanding of involved metabolic pathways. Eight volunteers daily consumed 400 mL of juice for four consecutive days and urine samples were collected before intake and 24h after each citrus juice intake. Urine samples were analysed by nanoHPLC-q-TOF, followed by principal component analysis (PCA) and Student's t-test (p<0.05). PCA showed a separation between two groups (before and after citrus juice consumption). This approach allowed the identification of four endocrine compounds (tetrahydroaldosterone-3-glucuronide, cortolone-3-glucuronide, testosterone-glucuronide and 17-hydroxyprogesterone), which belonged to the steroid biosynthesis pathway as significant metabolites upregulated by citrus juice intake. Additionally, these results confirmed the importance of using the non-targeted metabolomics technique to identify new endogenous metabolites, up- or down-regulated as a consequence of food intake.

Quantum optical response of metallic nanoparticles and dimers.

The optical properties of metallic nanoparticles (NPs) can be described with analytical models based on fundamental quantum mechanical principles, of which the Drude model constitutes the classical limit. Here, we examine the plasmonic properties of silver and gold nanospheres and dimers, with radii ranging from 10 to 1 nm, extending from the classically described regime to the quantum size regime. We have studied the spectral extinction cross section by using the T-matrix method. The results indicate an increasingly substantial change in NP permittivity as the radius is reduced below 5 nm, showing a clear blueshift and weakening of the plasmon resonances for both silver and gold. As a consequence, we observe a dramatic change in the interaction of dimers, especially in the case of gold, where the introduction of quantum mechanically corrected optical properties quenches the plasmonic resonance and predicts an absence of the expected associated redshift.

Physical activity increases the bioavailability of flavanones after dietary aronia-citrus juice intake in triathletes.

Control and triathlete volunteers (n=8 and n=15, respectively) were given 400 mL and 200 mL of aronia-citrus juice (AC-juice), respectively. The 24h urine samples were hydrolysed to determine the flavanones concentration by UPLC-QqQ-MS/MS. The flavanones metabolites in both groups of volunteers were glucuronides, sulfates, and sulfo-glucuronides, and the total excretion of flavanones increased fivefold in the triathletes compared with the control volunteers. The increase of ninefold in the homoeriodictyol of triathletes compared to control volunteers may suggest the overactivation of the microbiota metabolism caused by physical exercise. No differences concerning the bioavailability were detected between men and women in controlboth groups. The AC-juice could provide synergistic effects on health due to the increase in the bioavailability of flavanones, avoiding the deleterious effects caused by the overdosage of nutritional supplements.

Nimodipine inhibits IL-1ß release stimulated by amyloid ß from microglia.

BACKGROUND AND PURPOSE: There is growing evidence that inflammation plays a major role in the pathogenesis of neural damage caused by deposition of amyloid ß (Aß) in the brain. Nimodipine has received attention as a drug that might improve learning and reduce cognitive deficits in Alzheimer's disease, but the mechanism of action is poorly known. In this study, we tested the hypothesis that nimodipine inhibited Aß-stimulated IL-1ß release from microglia. EXPERIMENTAL APPROACH: Cultures of N13 microglia cells or primary mouse microglia were treated with nimodipine, and intracellular accumulation and release of IL-1ß in response to Aß or to the P2 receptor agonists ATP and benzoyl ATP (BzATP) were measured. Accumulation of IL-1ß was measured in vivo after intrahippocampal inoculation of Aß in the absence or presence of nimodipine. The effect of nimodipine on Aß-triggered cytotoxicity was also investigated. KEY RESULTS: We show here that nimodipine dose-dependently inhibited Aß-stimulated IL-1ß synthesis and release from primary microglia and microglia cell lines. Furthermore, nimodipine also inhibited Aß-induced IL-1ßin vivo accumulation at concentrations known to be reached in the CNS. Finally, nimodipine protected microglia from Aß-dependent cytotoxicity. CONCLUSION AND IMPLICATIONS: These data suggest that alleviation of symptoms of Alzheimer's disease following nimodipine administration might be due to an anti-inflammatory effect and point to a novel role for nimodipine as a centrally acting anti-inflammatory drug.

A ultra-pressure liquid chromatography/triple quadrupole tandem mass spectrometry method for the analysis of 13 eicosanoids in human urine and quantitative 24 hour values in healthy volunteers in a controlled constant diet.

RATIONALE: Isoprostanes (IsoPs) are a series of prostaglandin (PG)-like compounds formed non-enzymatically through free-radical-induced peroxidation of arachidonic acid. They are considered as 'gold-standard' biomarkers for oxidative stress, in general, and lipid peroxidation, in particular. METHODS: A new qualitative and quantitative analytical method for the determination of 13 eicosanoids in human urine using solid-phase extraction (SPE) and ultra-pressure liquid chromatography coupled to tandem mass spectrometry (UPLC/MS/MS) has been developed. The SPE was optimized by comparison of the extraction efficiency and recoveries of three distinct cartridges: Strata X-AW, C18 Sep-Pak, and Oasis HLB. The UPLC/MS/MS approach in the multiple reaction monitoring (MRM) mode was developed using negative electrospray ionization (ESI). RESULTS: The validated method provides a high-throughput assay with an adequate linearity from 0.16 to 330 ng mL(-1). The limit of detection (LOD) and limit of quantification (LOQ) for each analyte showed low intervals (0.021-0.64 ng mL(-1) and 0.042-1.28 ng mL(-1), respectively). Urinary IsoPs were determined in 24 healthy volunteers and ranged from 685 to 3480 ng 24 h(-1) and from 864 to 7511 ng 24 h(-1) in urine from women and men, respectively. CONCLUSIONS: This analytical method could constitute a useful tool for the determination of oxidative stress biomarkers in clinical studies in which IsoPs may evidence early pathological conditions, as suggested by the determination of the baseline IsoPs content in human urine, since it improves upon the detection capacity of previously described methods. The quantity of IsoPs excreted in urine was higher than that found in previous reports due to the total hydrolysis of the conjugated forms.

Síndrome de cauda equina secundaria a hernia discal lumbar gigante / Cauda equina syndrome secondary to giant lumbar disc herniation

Objetivo: Relacionar los hallazgos clínicos de presentación de síndrome de cauda equina secundario a hernia discal lumbar con el resultado final en cinco pacientes. Pacientes y metodología: Presentamos una serie de 5 pacientes con SCE secundario a hernia discal lumbar gigante confirmado por RMN, sometidos a discectomía más descomprensión temprana del canal medular. Resultados: La descompresión quirúrgica se realizó de forma urgente, todas las pacientes presentaban trastornos viscerales al ingreso. A pesar del tratamiento precoz, solo tres pacientes experimentaron una recuperación completa de la disfunción visceral y de los déficits motor y sensitivo previos. Conclusiones: la descompresión precoz, en ningún caso va en detrimento del pronóstico, y puede ser de ayuda para prevenir que una lesión incompleta progrese hacia una parálisis completa de esfínteres (AU)

Objetive: To determine the relation between clinical symptoms at presentation and the outcomes in a group of patients with CES secondary lumbar disc herniation. Patients and method: We present 5 patients with CES secondary to a giant lumbar disc herniation confirmed by MRI, submitted to discectomy and early decompression of the spinal canal. Results: Urgent surgical decompression was performed, all the patients were presenting visceral dysfunction on admission. Despite early treatment, only three patients have experienced a complete recovery of the visceral dysfunction and motor and sensory previous symptoms. Conclusions: The influence in a delay in the surgical treatment may well be overestimated due to the classic recommendations of the first published studies. However, early decompression is not detrimental, and may help to prevent an incomplete lesion progressing to complete sphincter paralysis (AU)

[Dietary iron intake and deficiency in elite women volleyball players]. / Ingesta dietética de hierro y su deficiencia en las jugadoras de voleibol femenino de élite.

INTRODUCTION: Volleyball practice requires repeated impacts on arms and feet caused by vertical jumps, falls, auctions, sudden and rapid changes of direction, which is why might raise us problems in the metabolism of iron (Fe) and the recommended intake of 18 mg/day (in the women in general), is not sufficient to meet the needs of the players of volleyball female (JVF). OBJECTIVE: We analyzed the FS and IST of 10 JVF a team of Spanish SuperLeague (26.6 ± 5.9 years and height 178.05 ± 8.7 cm) in two moments of the season: Week 0 (pre-start of preseason) and week 11 (after 11 weeks of training and 6 games of the regular season). Also calculated Fe intake in this period with consumption frequency questionnaire developed and tested with food dietary records of 7 days. RESULTS: We observed that an intake of 25.8 mg/day of dietary Fe is not sufficient to prevent 30% of the JVF suffer pre-latent iron deficiency and 20% latent deficit (pre-anemia). CONCLUSION: It could be recommended conducting periodic blood analytical and a food education, teaching which foods containing a high content of Fe-type heme, and the factors that can interfere with absorption.

Polar decomposition of the Mueller matrix: a polarimetric rule of thumb for square-profile surface structure recognition.

In this research, the polar decomposition (PD) method is applied to experimental Mueller matrices (MMs) measured on two-dimensional microstructured surfaces. Polarization information is expressed through a set of parameters of easier physical interpretation. It is shown that evaluating the first derivative of the retardation parameter, δ, a clear indication of the presence of defects either built on or dug in the scattering flat surface (a silicon wafer in our case) can be obtained. Although the rule of thumb thus obtained is established through PD, it can be easily implemented on conventional surface polarimetry. These results constitute an example of the capabilities of the PD approach to MM analysis, and show a direct application in surface characterization.

Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442.

The PhaF is a nucleoid-associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; the N-terminal PHA granule-binding domain (phasin domain) and the C-terminal half containing AAKP-like tandem repeats characteristic of the histone H1 family. Although the PhaF binding to PHA granules and its role as transcriptional regulator have been previously demonstrated, the cell physiology meaning of these properties remains unknown. This work demonstrates that PhaF plays a crucial role in granule localization within the cell. TEM and flow cytometry studies of cells producing granules at early growth stage demonstrated that PhaF directs the PHA granules to the centre of the cells, forming a characteristic needle array. Our studies demonstrated the existence of two markedly different cell populations in the strain lacking PhaF protein, i.e. cells with and without PHA. Complementation studies definitively demonstrated a key role of PhaF in granule segregation during the cell division ensuring the equal distribution of granules between daughter cells. In vitro studies showed that PhaF binds DNA through its C-terminal domain in a non-specific manner. All these findings suggested a main role of PhaF in PHA apparatus through interactions with the segregating chromosome.

Ordered metal nanotube arrays fabricated by PVD.

In this work we report a simple method to fabricate ordered arrays of metal nanotubes. This method is based on the deposition of a metal by PVD onto an anodized aluminum oxide (AAO) template. The dimensions of the synthesized nanotubes depend both on the AAO template and on the deposited metal. In fact, it is observed that the aspect ratios of the nanotubes clearly depend significantly on the metal, ranging from 0.6 (Fe) to at least 3 (Zr).

Dolor lumbar crónico de origen facetario. Resultado del tratamiento mediante rizolisis percutánea. Selección de pacientes y técnica quirúrgica / Chronic lumbar facet joint pain. Treatment results using percutaneous rhizolysis. Patient selection and surgical technique

Objetivo: Evaluar la eficacia del tratamiento del dolor lumbar crónico de origen facetario mediante denervación percutánea facetaria por radiofrecuencia (rizolisis) para reducir el dolor y mejorar la discapacidad asociada en un grupo de pacientes meticulosamente seleccionados mediante combinación de la clínica, la exploración, las pruebas por imagen y el bloqueo anestésico facetario, y compararlo con la literatura médica previa. Material y métodos Estudio prospectivo de 70 pacientes con dolor lumbar crónico facetario de más de 3 meses de evolución, que no mejoraron con tratamiento conservador. Pacientes valorados clínicamente siguiendo indicaciones del Grupo para el Estudio de las Enfermedades del Raquis (GEER). Edad media: 49,52 años. Duración media del dolor: 6,86 años. Seguimiento mínimo: un año. Resultados Tras la rizolisis, el dolor lumbar medido mediante escala analógica visual (EAV) disminuye significativamente (p<0,05). Igualmente, mejoran de forma significativa los valores para el cuestionario Oswestry de discapacidad (ODI), reflejando una notable mejoría en su calidad de vida (p<0,05) al disminuir su incapacidad por el dolor lumbar. El 91,42% de los pacientes experimenta alivio significativo del dolor tras rizolisis, que llega a ser superior o igual a 6 meses en el 61,4% de los pacientes. Al año, el 84,5% de los pacientes afirma que volvería a recibir el mismo tratamiento. Conclusiones La rizolisis es una valiosa herramienta para el tratamiento sintomático del dolor lumbar crónico facetario. Una meticulosa selección de los pacientes mediante la combinación de la clínica, la exploración, las pruebas por imagen y el bloqueo anestésico facetario obtiene resultados duraderos en el tiempo ayudando a disminuir en estos pacientes su incapacidad por el dolor lumbar(AU)

Purpose: To evaluate the efficacy of percutaneous radiofrequency zygapophysial joint neurotomy (rhizotomy) to decrease pain and improve associated disability, in a group of patients suffering from low back pain from facet joint origin meticulously selected on the basis of a combination of by clinical findings, physical examination, imaging tests and anaesthetic diagnostic blocks. Materials and methods Prospective study with 70 patients treated with rhizotomy for low back pain from facet joint origin of longer than three months who did not improved after conservative treatment. Patients evaluated following the guidelines of the Spanish Society for the Study of the Spinal Diseases (GEER). Mean age was 49.52 years. Mean duration of pain: 6.86 years. Minimum follow-up: one year. Results Following percutaneous rhizotomy, mean low back pain as measured on the Visual Analogue Scale (VAS), decreased significantly (p<0.05). Likewise, there was a significant improvement in the values given for the Owestry Disability Index (ODI) reflecting a notable improvement in terms of quality of life (p<0.05). Following rhizotomy 91.4% of patients reported significant pain relief, wich was higher than or equal to six month in 61.4% of patients. At one year, 84.5% of patients stated that they would undergo the same treatment again, showing high satisfaction with the treatment received. Conclusions Percutaneous rhizotomy can be considered a valuable treatment for the symptomatic relief of chronic low back pain from facet joint origin. Meticulous patient selection by combining clinical and physical findings, imaging tests and anaesthetic diagnostic blocks, provides significant and lasting pain relieve, contributing to a reduction in the associated disability in patients suffering from chronic low back pain(AU)

Post-synthesis alignment of chemically modified carbon nanotubes in magnetic fields.

In this paper we present an improved procedure to prepare carbon nanotubes bundles functionalized with magnetite nanoparticles. Carbon nanotubes (CNTs) have been modified by hydrophobic adsorption of a carboxylic acid derivative and the previously synthesized magnetite nanoparticles have been attached to the acid groups. Electron microscopy studies show a high density of magnetite nanoparticles on the surface of CNTs. These modified carbon nanotubes become magnetic and can be appropriately oriented by using external magnetic fields.

Characterization of nitrogen-doped carbon nanotubes by atomic force microscopy, X-ray photoelectron spectroscopy and X-ray absorption near edge spectroscopy.

In this work, we perform a comparative study on single-walled carbon nanotubes (CNT) before and after low energy nitrogen ion bombardment (70 eV and 25 x 10-6 A/cm2) at room temperature. The morphology and the mechanical properties were studied by Atomic Force Microscopy (AFM). The bonding configuration of the N-doped CNTs was established by X-ray Photoelectron Spectroscopy (XPS) and X-ray Absorption Near Edge Spectroscopy (XANES). Single-walled carbon nanotubes were prepared using non-intrusive methods and deposited onto silicon substrates. For the spectroscopic studies, samples with a high concentration of CNTs were analyzed. XPS reveals different chemical states for carbon related to the incorporation of nitrogen. XANES confirms the hexagonal structure of the CNTs, resembling the bonding structure of hexagonal carbon nitrides. AFM images confirm that the CNTs were not destroyed after low energy N2+. The morphology of the original nanotubes maintains after nitrogen ion bombardment except for the incorporation of some pearl-shaped decoration, probably due to the adsorption of some contaminants or to deposition of re-sputtered material. Whereas CNTs improve their adherence to the substrate, this extra granularity on the CNT is easily removed even with the AFM tip. In conclusion, spectroscopic measurements and mechanical properties made clear information on the changes produced on CNT after nitrogen incorporation.

Attachment of magnetite nanoparticles on carbon nanotubes bundles and their response to magnetic fields.

We present an alternative method to control the alignment of carbon nanotube bundles by applying an external magnetic field on magnetic nanoparticles functionalized nanotubes. Carbon nanotubes have been modified by pi-pi stacking of a carboxylic acid and the previously synthesized Fe3O4 nanoparticles have been bounded to the acid groups. Results obtained by TEM, SEM and FESEM show that the magnetite nanoparticles are distributed along the nanotube surfaces, showing a high dispersion and a mean particle size of ca. 4-5 nm. In presence of a magnetic field the modified nanotube bundles have been clearly oriented along the axis parallel to the field.

Nanoscopic surface inspection by analyzing the linear polarization degree of the scattered light.

We present an optical method for the nanoscopic inspection of surfaces. The method is based on the spectral and polarization analysis of the light scattered by a probe nanoparticle close to the inspected surface. We explore the sensitivity to changes either in the probe-surface distance or in the refractive index of the surface.

AqquaScan: design and implementation of an internet-based service for the remote monitoring and management of decentralised WWTPs.

This paper describes the design and implementation of AqquaScan, an Internet-based service for remote monitoring and integrated management of decentralised WWTPs. AqquaScan is a multi-user and multi-WWTP service. It has been built according to criteria such as flexibility, scalability and interoperability with the idea of providing an open environment suited to quickly accommodate future scenarios (e.g. incorporation of new plants or upgrading of existing installations). Both, the management of plant information and users interfaces have been implemented in distributed software components that communicate with one another via web services. The implemented web services can be exploited to develop customised user interfaces for visualising the monitored data. By default, a customised web-based client module has been programmed in order for users to be able to exploit the facilities offered within AqquaScan: (1) real-time monitoring of on-line signals; (2) visualisation of historical data; (3) changing operational parameters; (4) notification of time-event information; and (5) storage of measurements from laboratory analysis. At present, AqquaScan is fully operative and is offering supervision services to eleven industrial WWTPs distributed around Northern Spain.

Crown Rot of Zucchini Squash Caused by Fusarium solani f. sp. cucurbitae in Almería Province, Spain.

Cucumber, melon, watermelon, and zucchini are intensively cropped in the southern part of Spain where approximately 20,000 ha of the crops are grown in greenhouses. In the spring of 2007, zucchini plants (Cucurbita pepo) at the fruit-bearing stage in three commercial plastichouses in Almería exhibited necrosis on the basal stem, wilt, and death. The incidence of dead plants was 20 to 30%. Fusarium solani was consistently isolated from the basal stems of symptomatic plants on potato dextrose agar (PDA). Cultures of six single-hyphal transfers were identified on the basis of molecular sequences and morphological characteristics (2). Sequences of ribosomal DNA from ITS1 region, 5.8S rDNA, and ITS2 were identical for all six isolates of F. solani. The rDNA sequence of isolate Fscl-3 of F. solani was deposited as GenBank Accession No. AM940070. The pathogenicity of these six isolates of F. solani was tested in two experiments conducted in one plastichouse in Almería. Pregerminated seeds of zucchini cv. Consul were sown in 1-liter containers filled with vermiculite on 21 May and 22 June, 2007 (experiments 1 and 2, respectively). Plants at the one- to two-true-leaf stage or younger were inoculated with a soil drench of 2.0 to 8.4 × 105 propagules per ml). One colonized PDA petri plate of each isolate was blended and homogenized in 500 ml of distilled water. Inoculum (50 ml per plant) was poured around the stem of zucchini plants growing in vermiculite. The experimental design was a randomized complete block with three replicates with each plot comprising four plants (one plant per container). In both experiments, 12 uninoculated plants of the same cultivar served as controls. Plants were maintained for 1 month following inoculation in a greenhouse with mean temperatures ranging between 20.7 and 24.6°C and 23.3 to 29.8°C for experiments 1 and 2, respectively. Wilting first occurred 9 days after inoculation, and 14 days later, all plants inoculated with the F. solani isolates died. Inoculated plants exhibited lesions on the stem base without rot of secondary roots. At the end of the experiment, the uninoculated plants remained asymptomatic. Results of experiment 2, with higher temperatures, were similar. The pathogen was consistently recovered from symptomatic plants in both experiments, fulfilling Koch's postulates. Although F. solani f. sp. cucurbitae race 1 was reported in field squash (C. maxima) in the province of Valencia of east-central Spain (1), to our knowledge, this is the first report of F. solani as the causal agent of crown rot of zucchini plants in plastichouses in the Almería Province of Spain, one of the world's largest concentrations of greenhouses. References: (1) J. García-Jiménez et al. Plant Dis. 81:1216, 1997. (2) C. M. Messiaen and R. Cassini. Taxonomy of Fusarium. Page 427 in: Fusarium: Diseases, Biology, and Taxonomy. P. E. Nelson et al., eds. Pennsylvania State University, University Park, 1981.