Subject(s)
Allergens/immunology , Respiratory Hypersensitivity/diagnosis , Respiratory Hypersensitivity/immunology , Adolescent , Adult , Allergens/chemistry , Animals , Bronchial Provocation Tests , Child , Cricetinae , Female , Humans , Male , Middle Aged , Phodopus/immunology , Respiratory Hypersensitivity/chemically induced , Respiratory Hypersensitivity/pathology , Skin TestsABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Child , Adolescent , Young Adult , Adult , Middle Aged , Pets/immunology , Phodopus/immunology , Allergy and Immunology , Hypersensitivity/complications , Hypersensitivity/diagnosis , Hypersensitivity/immunology , Conjunctivitis/complications , Conjunctivitis/immunology , Conjunctivitis, Allergic/immunology , Mesocricetus/immunology , Nasal Obstruction/complications , Nasal Obstruction/immunology , Rhinitis/complications , Rhinitis/immunologySubject(s)
Allergens/immunology , Antigens, Plant/immunology , Food Hypersensitivity/diagnosis , Plant Proteins/immunology , Salvia/immunology , Seeds/immunology , Allergens/analysis , Anaphylaxis/blood , Anaphylaxis/diagnosis , Anaphylaxis/immunology , Antigens, Plant/analysis , Food Hypersensitivity/blood , Food Hypersensitivity/immunology , Humans , Immunoglobulin E/blood , Immunoglobulin E/immunology , Male , Middle Aged , Plant Extracts/chemistry , Plant Proteins/analysis , Seeds/chemistryABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Seeds/cytology , Seeds/enzymology , Rhinitis, Allergic, Seasonal/diagnosis , Rhinitis, Allergic, Seasonal/metabolism , Diet/methods , Urticaria/pathology , Seeds/metabolism , Rhinitis, Allergic, Seasonal/complications , Diet , Urticaria/prevention & controlABSTRACT
BACKGROUND: The relevance of contact allergy to plant-related food has recently emerged. Oral allergy syndrome is one of the most characteristic symptoms of fruit allergy, although it also causes systemic reactions. Plant-food allergy is increasing at the same time as pollen allergy, and fruit-induced allergic contact urticaria could be rising as well. OBJECTIVES: The present study was carried out in order to investigate whether one particular primary melon-peel allergen is responsible for contact urticaria. METHODS: Fourteen patients presenting with contact urticaria after touching melon peel were evaluated. A melon-peel extract was prepared and analysed by immunoblotting using the patients' sera. Molecular characterization of IgE-binding bands was performed using mass spectrometry. Melon-peel lipid transfer protein (LTP) was purified. Inhibition studies and contact challenge with the protein were performed to confirm IgE reactivity to the purified allergen. RESULTS: An IgE-binding band of ~8-9 kDa was observed in an immunoblotting assay with all the patients' sera and was identified as an LTP. The melon-peel LTP was purified in two chromatography steps. Inhibition studies confirmed LTP as a major allergen in patients with melon-peel contact urticaria. Contact challenge with melon-peel LTP was performed in five patients, all of whom had positive results, exhibiting itchy erythema and hives in the area of contact. CONCLUSIONS: This study confirmed our previous findings that melon-peel LTP is a major allergen and is responsible for contact allergy. This knowledge may be used to improve both diagnosis and treatment of patients allergic to melon.
