ABSTRACT
In this work a novel approach to identify new therapeutic targets consisting of serum proteins which contain an oligonucleotide binding domain is presented.
Subject(s)
Blood Proteins/genetics , Oligodeoxyribonucleotides/genetics , Oligodeoxyribonucleotides/pharmacology , Binding Sites , Blood Proteins/antagonists & inhibitors , Blood Proteins/chemistry , Drug Design , Gene Targeting , HMGB1 Protein/antagonists & inhibitors , HMGB1 Protein/chemistry , HMGB1 Protein/genetics , Humans , Macromolecular Substances , Models, Molecular , Oligodeoxyribonucleotides/chemistryABSTRACT
In this work we explore the ability of a chimeric LNA/DNA bent duplex, in which the kink is induced by 2 unpaired adenines in the middle of one strand, to bind HMGB1, a protein involved in many inflammatory processes. The LNA/DNA duplex was compared with the corresponding full DNA and PNA/DNA chimera duplexes from a thermodynamic and spectroscopic point of view.
Subject(s)
DNA/chemistry , DNA/pharmacology , HMGB1 Protein/antagonists & inhibitors , Oligonucleotides/chemistry , Oligonucleotides/pharmacology , HumansABSTRACT
In the present work, we report the synthesis and the characterization of a new chiral nucleoaminoacid, in which a diaminobutyric moiety is connected to the DNA nucleobase by an amidic bond, and its oligomerization to give the corresponding nucleo-gamma-peptide. The ability of this synthetic polymer to bind complementary DNA was studied in order to explore its possible use in antigene/antisense or diagnostic applications. Our interest in the presented DNA analogue was also supported by the importance of gamma-aminoacid-containing compounds in natural products of biological activity and by the known stability of gamma-peptides to enzymatic degradation. Furthermore, our work could contribute to the study of the role of nucleopeptides as prebiotic material in a PNA world that could successively lead to the actual DNA/RNA/protein world, as recently assumed.
Subject(s)
Aminobutyrates/chemistry , Peptide Nucleic Acids/chemistry , Base Sequence , Chromatography, High Pressure Liquid , Circular Dichroism , Molecular Conformation , Molecular Structure , Nucleic Acid Probes/chemical synthesis , Nucleic Acid Probes/chemistry , Peptide Nucleic Acids/chemical synthesis , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Ultraviolet , TemperatureABSTRACT
Los autores estudian la membrana alveolo-dental en 10 casos de esclerodermia sistemica progresiva. Dos pacientes eran desdentados.Actualizan el concepto de membrana alveolo-dental y sus componentes normales. Se sintetizan los resultados en dos tablas senalando la edad promedio de 44,7 anos. El engrosamiento de la membrana alveolo dentaria radiologicamente hallado fue de un promedio del 86%. Se hace una revision bibliografica sobre los trabajos relacionados con el tema y se plantean inquietudes futuras
Subject(s)
Scleroderma, Systemic , Periodontal LigamentABSTRACT
Los autores estudian la membrana alveolo-dental en 10 casos de esclerodermia sistemica progresiva. Dos pacientes eran desdentados.Actualizan el concepto de membrana alveolo-dental y sus componentes normales. Se sintetizan los resultados en dos tablas senalando la edad promedio de 44,7 anos. El engrosamiento de la membrana alveolo dentaria radiologicamente hallado fue de un promedio del 86%. Se hace una revision bibliografica sobre los trabajos relacionados con el tema y se plantean inquietudes futuras
