A "turn-off" photoluminescent sensor for H2O2 detection based on a zinc oxide-graphene quantum dot (ZnO-GQD) nanocomposite and the role of amine in the development of GQD.

In this work, graphene quantum dots (GQD) were prepared through a hydrothermal process. The photoluminescence (PL) emission spectrum for GQD prepared with high NH4OH concentration (sample D1-t) was attained at lower wavelength (406 nm), compared to GQD synthesized with low NH4OH concentration (sample D2-t attained at 418 nm). From these results, a smaller particle size for D1-t was deduced; according to TEM images the GQD particles are around 5 nm. The Raman ID3/IG ratio which is related to C-O groups at the edges of GQD and the full width at half maximum was lower for D1-t than D2-t. This was ascribed to the amine group incorporation at the edges and at the basal planes in D1-t, whilst in D2-t they prefer principally the edges of the GQD structure. The ZnO nanoparticles bonded to GQD (ZnO-GQD, nanocomposites) enhance the PL emission intensity. The H2O2 detection tested by photoluminescence spectroscopy, was found to occur thanks to the ZnO from the nanocomposite and its interaction with H2O2, producing a quenching effect. This quenching was accentuated by the increase of the H2O2 concentration. Such properties suggest the ZnO-GQD nanocomposite as a candidate to be used as a sensor material.

Discordant results for ALK based on immunohistochemistry versus fluorescence in situ hybridization in a cohort of patients diagnosed with lung adenocarcinoma / Discordancia de los resultados para ALK basados en inmunohistoquímica e hibridación fluorescente in situ en una cohorte de pacientes diagnosticados con adenocarcinoma de pulmón

Introduction: Anaplastic lymphoma kinase (ALK) rearrangement located on the short arm of chromosome 2, region 2 and band 3 is frequent in lung cancer patients who respond to targeted therapies with ALK inhibitors Therefore, their identification has become a standard diagnostic test in patients with advanced NSCLS, as such chromosomal alterations may lead to the activation of important signalling pathways involved in cell survival and proliferation. Methods: To investigate the ALK gene status, we performed FISH and IHC assays in 18 lung adenocarcinoma patients, 12 women and 6 men, aged between 29 and 85 years. Paraffin-embedded samples were analyzed in the Pathology Department of the Hospital Universitario San Ignacio. Results: Results between the two techniques in 5 patients showed discordant patterns, being positive for FISH and negative for IHC. The borderline to define ALK positivity was set at 15%, These results present experimental evidence that the techniques differ in specific situations. Conclusions: Our findings show that it is advisable to investigate the ALK gene status in patients with suspected lung cancer using both FISH and IHC in combination.(AU)

Introducción: La reorganización de la (anaplastic lymphoma kinase) ALK ubicada en el brazo corto del cromosoma 2, región 2 y banda 3 es frecuente en los pacientes con cáncer de pulmón que responden a terapias dirigidas con inhibidores de la ALK. Por ello, su identificación se ha establecido como una prueba diagnóstica estándar en pacientes con CPCNP, ya que dichas alteraciones cromosómicas puedan determinar la activación de importantes vías de señalización implicadas en la supervivencia y proliferación celulares. Métodos: Para determinar el estatus de gen ALK se realizaron pruebas FISH e IHC en 18 pacientes con adenocarcinoma pulmonar, 12 mujeres y 6 varones, con edades comprendidas entre 29 y 85 años. Las muestras fueron analizadas en el Departamento de Anatomía Patológica del Hospital Universitario San Ignacio. Resultados: Los resultados entre ambas técnicas mostraron patrones discordantes en 5 pacientes, con positividad de FISH y negatividad con IHC. El límite para definir la positividad de ALK se estableció en el 15%. Estos resultados muestran evidencia experimental que dichas técnicas difieren en situaciones específicas. Conclusiones: Este estudio recomienda la investigación del estatus del gen ALK en los pacientes con sospecha de cáncer de pulmón, mediante la combinación de FISH e IHC.(AU)

Evaluating the complete (44-item), short (20-item) and ultra-short (10-item) versions of the Big Five Inventory (BFI) in the Brazilian population.

The Big Five Inventory (BFI) is an instrument designed to assess the personality of individuals aged 18 and above. The original version consists of 44 items divided into five sub-scales representing each of the five personality factors: agreeableness, neuroticism, conscientiousness, openness, and extraversion. The main purpose of this study was to assess the factorial structure of the 44-item BFI and the reliability of two shorter versions with 20 and 10 items. The study also aimed to present normative data for interpreting scores from the short and ultrashort versions of the BFI for the Brazilian population. A total of 3565 individuals with a mean age of 33.3 years (SD = 13.0) from all Brazilian states participated in the study, with 44.2% from the State of Rio Grande do Sul. Participants completed a sociodemographic questionnaire and the BFI. Confirmatory factor analysis showed poor adaptation of the original 44-item model, but the short and ultrashort versions with 20 and 10 items respectively had good adaptation indexes and reliability, with Omega coefficients above 0.70. Normative data for the shorter versions were presented using mean, standard deviation, and percentiles (lower, medium, and higher). The study concluded that the short and ultrashort versions of the BFI have good reliability and can be used in surveys requiring a brief personality assessment.

Discordant results for ALK based on immunohistochemistry versus fluorescence in situ hybridization in a cohort of patients diagnosed with lung adenocarcinoma.

INTRODUCTION: Anaplastic lymphoma kinase (ALK) rearrangement located on the short arm of chromosome 2, region 2 and band 3 is frequent in lung cancer patients who respond to targeted therapies with ALK inhibitors Therefore, their identification has become a standard diagnostic test in patients with advanced NSCLS, as such chromosomal alterations may lead to the activation of important signalling pathways involved in cell survival and proliferation. METHODS: To investigate the ALK gene status, we performed FISH and IHC assays in 18 lung adenocarcinoma patients, 12 women and 6 men, aged between 29 and 85 years. Paraffin-embedded samples were analyzed in the Pathology Department of the Hospital Universitario San Ignacio. RESULTS: Results between the two techniques in 5 patients showed discordant patterns, being positive for FISH and negative for IHC. The borderline to define ALK positivity was set at 15%, These results present experimental evidence that the techniques differ in specific situations. CONCLUSIONS: Our findings show that it is advisable to investigate the ALK gene status in patients with suspected lung cancer using both FISH and IHC in combination.

Microbiological and microstructural analysis of indwelling bladder catheters and urinary tract infection prevention.

OBJECTIVE: To analyze the microbiological and microstructural part of indwelling urinary catheters and their association with urinary tract infection prevention. METHOD: This is a cross-sectional study, from June to December 2020, in which 42 indwelling urinary catheter tips and sterile urine samples were collected for analysis of crystals in optical microscopy and biofilms in scanning electron microscopy. Culture analysis and specification of the type of bacteria were performed. RESULTS: It was found that 35.71% of the samples had mature biofilm adhered to the catheter tip. Biofilms of Proteus mirabilis, Enterococcus faecalis, Staphylococcus epidermidis, Enterococcus faecium and Enterobacter cloacae stood out. The presence of magnesium-ammonium-phosphate crystal was associated with the presence of urinary tract infection and with Proteus mirabilis. There was a significant association (p = 0.001) between the use of prophylactic antibiotics versus urine culture >105 CFU/mL. CONCLUSION: The analyzes contributed to clinical practice, as it reinforces the development of effective and monitored strategies on cultures and urinary tract infection prevention associated with indwelling urinary catheters.

Microbiological and microstructural analysis of indwelling bladder catheters and urinary tract infection prevention / Análisis microbiológico y microestructural de sondas vesicales permanentes y prevención de infecciones del tracto urinario / Análise microbiológica e microestrutural dos cateteres vesicais de demora e prevenção de infecção do trato urinário

ABSTRACT Objective: To analyze the microbiological and microstructural part of indwelling urinary catheters and their association with urinary tract infection prevention. Method: This is a cross-sectional study, from June to December 2020, in which 42 indwelling urinary catheter tips and sterile urine samples were collected for analysis of crystals in optical microscopy and biofilms in scanning electron microscopy. Culture analysis and specification of the type of bacteria were performed. Results: It was found that 35.71% of the samples had mature biofilm adhered to the catheter tip. Biofilms of Proteus mirabilis, Enterococcus faecalis, Staphylococcus epidermidis, Enterococcus faecium and Enterobacter cloacae stood out. The presence of magnesium-ammonium-phosphate crystal was associated with the presence of urinary tract infection and with Proteus mirabilis. There was a significant association (p = 0.001) between the use of prophylactic antibiotics versus urine culture >105 CFU/mL. Conclusion: The analyzes contributed to clinical practice, as it reinforces the development of effective and monitored strategies on cultures and urinary tract infection prevention associated with indwelling urinary catheters.

RESUMEN Objetivo: Analizar la parte microbiológica y microestructural de los catéteres urinarios permanentes y su asociación con la prevención de la infección del tracto urinario. Método: Transversal, de junio a diciembre de 2020, en el que se recolectaron 42 puntas de sonda urinaria permanente y muestras de orina estéril para análisis de cristales en microscopía óptica y biopelículas en microscopía electrónica de barrido. Se realizaron análisis de cultivo y especificación del tipo de bacteria. Resultados: Se encontró que el 35,71% de las muestras presentaban biopelícula madura adherida a la punta del catéter. Se destacaron las biopelículas de Proteus mirabilis, Enterococcus faecalis, Staphylococcus epidermidis, Enterococcus faecium y Enterobacter cloacae. La presencia de cristales de fosfato de magnesio-amonio se asoció con la presencia de infección del tracto urinario y con Proteus mirabilis. Hubo una asociación significativa (p = 0,001) entre el uso de antibióticos profilácticos versus urocultivo >105 UFC/mL. Conclusión: Los análisis contribuyeron a la práctica clínica, ya que refuerzan el desarrollo de estrategias efectivas y monitoreadas sobre cultivos y prevención de la infección del tracto urinario asociada a los catéteres urinarios permanentes.

RESUMO Objetivo: Analisar a parte microbiológica e microestrutural dos cateteres vesicais de demora e sua associação com a prevenção de infecção do trato urinário. Método: Transversal, entre junho e dezembro de 2020, em que foram coletadas 42 pontas cateteres vesicais de demora e amostras de urina estéril para análise de cristais em microscopia óptica e de biofilmes em microscopia eletrônica de varredura. Fez-se analise de cultura e especificação do tipo de bactérias. Resultados: Verificou-se que 35,71% das amostras apresentaram o biofilme maduro aderido à ponta do cateter. Destacaram-se biofilmes de Proteus mirabilis, Enterococcus faecalis, Staphylococcus epidermidis, Enterococcus faecium e Enterobacter cloacae. A presença do cristal magnésio-amônio-fosfato foi associada à presença de infecção do trato urinário e ao Proteus mirabilis. Verificou-se associação significativa (p = 0,001) entre o uso de antibiótico profilático versus a urocultura >105 UFC/mL. Conclusão: As análises contribuíram para a prática clínica, pois reforçam a elaboração de estratégias efetivas e monitoradas sobre culturas e prevenção de infecção do trato urinário associada ao cateter vesical de demora.

Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties.

The flavonoids are compounds synthesized by plants, and they have properties such as antioxidant, anticancer, anti-inflammatory, and antibacterial, among others. One of the most important bioactive properties of flavonoids is their antioxidant effect. Synthetic antioxidants have side toxic effects whilst natural antioxidants, such as flavonoids from natural sources, have relatively low toxicity. Therefore, it is important to incorporate flavonoids derived from natural sources in several products such as foods, cosmetics, and drugs. For this reason, there is currently a need to extract flavonoids from plant resources. In this review are described the most important parameters involved in the extraction of flavonoids by unconventional methods such as ultrasound, pressurized liquid extraction, mechanochemical, high hydrostatic pressure, supercritical fluid, negative pressure cavitation, intensification of vaporization by decompression to the vacuum, microwave, infrared, pulsed electric field, high-voltage electrical discharges, and enzyme-assisted extraction. There are no unified operation conditions to achieve high yields and purity. Notwithstanding, progress has been achieved in the development of more advanced and environmentally friendly methods of extraction. Although in literature are found important advances, a complete understanding of the extraction process in each of the unconventional techniques is needed to determine the thermodynamic and kinetic mechanisms that govern each of the techniques.

Mel e açúcar mascavo na cicatrização de feridas / Honey and brown sugar in wounds healing

O estudo teve como objetivo comparar a eficácia do mel e do açúcar mascavo na cicatrização de feridas induzidas cirurgicamente na pele de cobaias, para avaliar o tempo de cicatrização, as variações macroscópicas e microscópicas do tecido cicatricial, a contração das feridas e o grau de contaminação. Quinze cobaias da raça Abissínia, machos inteiros, de seis meses de idade e pesando entre 500 a 850 gramas, foram divididas em três grupos com cinco animais cada, nos quais foram induzidas, cirurgicamente, três feridas em cada animal com dimensão de 1,5cm² e distância entre elas de 1,5cm, na região dorsal. A ferida F1 foi tratada diariamente com mel, a ferida F2 com solução salina 0,9% (controle) e a ferida F3 com o açúcar mascavo. A eutanásia foi realizada no Grupo 1 ao sétimo dia após a cirurgia, no Grupo 2 ao décimo quarto dia e no Grupo 3 no vigésimo primeiro dia após a cirurgia, sendo as biópsias encaminhadas para avaliação histopatológica. Foram também realizados swabs das feridas em todos os grupos no terceiro e sétimo dias após a cirurgia e as amostras enviadas para a cultura e isolamento bacteriano. Concluiu-se que as feridas tratadas com o mel cicatrizaram mais rápido em relação às tratadas com o açúcar mascavo, demonstrando a maior propriedade cicatrizante do mel em relação ao açúcar mascavo.

The study aimed to compare the effectiveness of honey and brown sugar in wounds healing induced in the skin of guinea pigs, to evaluate the healing time, microscopic and macroscopic healing variations, wound contraction and contamination degree. Fifteen guinea pigs, Abyssinia breed, males, six months of age and weighing between 500 to 850 grams, were divided in three groups with five animals each. Three wounds were promoted in each animal, surgical, with 1.5cm² and 1.5cm between them, in the dorsal region. The F1 was treated, daily, with honey, F2 with saline solution 0,9% (control) and F3 with brown sugar. Euthanasia was performed in Group 1 on the seventh day after the surgery, Group 2 on the fourteenth day and the Group 3 on the twenty-first day after the surgery and the biopsies sent to histopathological evaluation. Wounds swabs were made in all groups on the third and seventh days after the surgery and the samples sent for culture and bacterial isolation. The wounds treated with honey healed faster than treated with brown sugar.

Diaqua-tris-(nitrato-κ(2)O,O'){2,2'-[pyridine-2,6-diylbis(methyl-ene-oxy)]dibenzaldehyde-κO(1)}dysprosium(III)-2,2'-[pyridine-2,6-diylbis(methyl-ene-oxy)]dibenzaldehyde (1/1).

The title compound, [Dy(NO(3))(3)(C(21)H(17)NO(4))(H(2)O)(2)]·C(21)H(17)NO(4), may be considered as an organic-metalorganic 1:1 co-crystal, in which the two dialdehyde mol-ecules act as a ligand and as an organic moiety, respectively. The Dy(III) atom coordinates nine O atoms from the organic ligand, bidentate nitrate ions and water mol-ecules, approximating a square-face-tricapped trigonal-prismatic geometry. The coordinated dialdehyde is not planar: the uncoordinated oxybenzaldehyde group is twisted by 39.96 (4)° from the rest of the ligand. In contrast, the free organic moiety is almost planar, with an r.m.s. deviation of 0.15 Å. In the crystal, segregated stacks of dialdehyde are formed in the [100] direction. For the complex, the shortest π-π contact is found at 3.781 (2) Å, and for the free ligand, at 3.785 (2) Å. The crystal structure is further stabilized by O-H⋯O and O-H⋯N hydrogen bonds in which coordinated water mol-ecules are the donor groups.