ABSTRACT
Donepezil hydrochloride containing polyurethane/hydroxypropyl cellulose (PU/HPC) nanofibers were prepared by the electrospinning for transdermal drug delivery. PU/HPC nanofibers were characterized with SEM, DSC, and Pascal mercury porosimetry. Drug-excipient interaction was studied by ATR-FTIR. In vitro release of PU/HPC nanofiber mat (10:2:1) exhibited Korsmeyer-Peppas release kinetics controlled by the diffusion of drug. In vitro permeation studies across skin resembling synthetic membrane demonstrated the flux of model drug. The in vitro cytotoxicity data obtained via MTT assay indicated that PU/HPC nanofiber mat could be well tolerated by the skin and the components was not irritant for the skin.
Subject(s)
Cellulose/analogs & derivatives , Drug Carriers , Indans/metabolism , Nanofibers/chemistry , Nootropic Agents/metabolism , Piperidines/metabolism , Polyurethanes/chemistry , Animals , Cell Survival/drug effects , Cellulose/chemistry , Donepezil , Drug Compounding , Drug Liberation , Electrochemical Techniques , Indans/chemistry , Indans/pharmacology , Kinetics , Membranes, Artificial , Mice , Molecular Mimicry , NIH 3T3 Cells , Nanofibers/ultrastructure , Nootropic Agents/chemistry , Nootropic Agents/pharmacology , Permeability , Piperidines/chemistry , Piperidines/pharmacology , Skin/drug effects , Skin/metabolismABSTRACT
D- or L-aspartic acid containing polyacrylamides were synthesized. Binary and ternary complex formation between these polymers with copper and bovine serum albumin was studied by spectroscopic and electrochemical measurement. Depending on the ratio of the polymer/copper and protein/polymer, the mixture exhibited water-soluble and insoluble character. A hypothetical structural scheme for the formation of ternary complexes is proposed. The effect of radiation on these complexes was also investigated.
Subject(s)
Acrylic Resins/chemistry , Acrylic Resins/metabolism , Aspartic Acid/metabolism , Copper/metabolism , Serum Albumin, Bovine/metabolism , Cations, Divalent/metabolism , Chromatography, High Pressure Liquid , Conductometry , Hydrogen-Ion Concentration , Molecular Structure , Protein Binding , Pulse Radiolysis , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , Structure-Activity RelationshipABSTRACT
Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R2, R16)], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water. Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R2, R16) solution at pH 7. A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place. The immunogenic properties of PVP (R2, R16)-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed. 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse. However, a comparative study of immunogenic activity of polycomplexes versus BSA.E+incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production. In contrast to IFA+BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies. Such a carrier-directed response may be determined by increase in immunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes. Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities. Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.
