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Nutrients ; 10(9)2018 Sep 11.
Article in English | MEDLINE | ID: mdl-30208590

ABSTRACT

BACKGROUND: Chronic kidney disease and inflammation promote loss of Klotho expression. Given the well-established anti-inflammatory effects of omega-3 fatty acids, we aimed to investigate the effect of fish oil supplementation in a model of CKD. METHODS: Male C57BL/6 mice received supplementation with an adenine-enriched diet (AD, n = 5) or standard diet (CTL, n = 5) for 10 days. Two other experimental groups were kept under the adenine diet for 10 days. Following adenine withdrawal on the 11th day, the animals returned to a standard diet supplemented with fish oil (Post AD-Fish oil, n = 9) or not (Post AD-CTL, n = 9) for an additional period of 7 days. RESULTS: Adenine mice exhibited significantly higher mean serum urea, creatinine, and renal expression of the pro-inflammatory markers Interleukin-6 (IL-6), C-X-C motif chemokine 10 (CXCL10), and Interleukin-1ß (IL-1ß), in addition to prominent renal fibrosis and reduced renal Klotho gene expression compared to the control. Post AD-Fish oil animals demonstrated a significant reduction of IL-6, C-X-C motif chemokine 9 (CXCL9), and IL-1ß compared to Post AD-CTL animals. However, serum creatinine, renal fibrosis, and Klotho were not significantly different in the fish oil-treated group. Furthermore, renal histomorphological changes such as tubular dilatation and interstitial infiltration persisted despite treatment. CONCLUSIONS: Fish oil supplementation reduced renal pro-inflammatory markers but was not able to restore renal function nor Klotho expression in an adenine-induced CKD model.


Subject(s)
Adenine , Dietary Supplements , Fish Oils/administration & dosage , Inflammation Mediators/metabolism , Kidney/metabolism , Membrane Proteins/metabolism , Nephritis/diet therapy , Renal Insufficiency, Chronic/diet therapy , Animal Feed , Animals , Biomarkers/metabolism , Disease Models, Animal , Down-Regulation , Fibrosis , Kidney/pathology , Kidney/physiopathology , Klotho Proteins , Male , Mice, Inbred C57BL , Nephritis/chemically induced , Nephritis/metabolism , Nephritis/physiopathology , Renal Insufficiency, Chronic/chemically induced , Renal Insufficiency, Chronic/metabolism , Renal Insufficiency, Chronic/physiopathology
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