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2.
Vasc Med ; 14(3): 215-20, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19651670

ABSTRACT

Myeloperoxidase (MPO) is an enzymatic mediator of several inflammatory cascades and higher serum levels have been associated with increased risk of adverse cardiovascular events. We investigated the association of serum MPO with the ankle-brachial index (ABI) and peripheral arterial disease (PAD) in a bi-ethnic cohort of African-Americans and non-Hispanic white individuals. Participants included 1324 African-Americans (mean age 64 years, 71% women) and 1237 non-Hispanic white individuals (mean age 59 years, 57% women) belonging to hypertensive sibships. Serum levels of MPO were measured by solid phase sandwich immunoassay. ABI was measured using a standard protocol and PAD was defined as an ABI < 0.90. Multivariable regression analysis using generalized estimating equations were performed to assess whether serum MPO levels were associated with ABI and the presence of PAD. After adjustment for age and sex, higher MPO levels were significantly associated with lower ABI and the presence of PAD in African-Americans (p = 0.004 and p = 0.005, respectively) and in non-Hispanic white individuals (p = 0.001 and p = 0.016, respectively). After additional adjustment for conventional risk factors (diabetes, smoking status, total and high-density lipoprotein cholesterol, waist circumference, hypertension), prior history of myocardial infarction or stroke, and medication use (statins, aspirin, estrogen), higher MPO levels remained significantly associated with lower ABI and the presence of PAD in both African-Americans (p = 0.008 and p = 0.010, respectively) and non-Hispanic white individuals (p = 0.001 and p = 0.018, respectively). We conclude that higher MPO levels are associated with lower ABI and the presence of PAD in African-Americans and non-Hispanic white individuals.


Subject(s)
Ankle/blood supply , Blood Pressure , Brachial Artery/physiopathology , Peripheral Vascular Diseases/diagnosis , Peroxidase/blood , Black or African American/statistics & numerical data , Aged , Biomarkers/blood , Cross-Sectional Studies , Down-Regulation , Female , Humans , Male , Middle Aged , Peripheral Vascular Diseases/enzymology , Peripheral Vascular Diseases/ethnology , Peripheral Vascular Diseases/physiopathology , Predictive Value of Tests , ROC Curve , Regression Analysis , Risk Assessment , Risk Factors , United States/epidemiology , White People/statistics & numerical data
3.
Chest ; 125(4): 1483-91, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15078762

ABSTRACT

STUDY OBJECTIVE: s: The impairment of cardiac contractility during endotoxemia involves induction of nitric oxide formation through a cascade of events initiated by overexpression of proinflammatory cytokines. We previously showed that hypothermia attenuates endotoxin-induced overexpression of nitric oxide in rat lungs. In the present study, we tested the hypothesis that hypothermia protects against endotoxin-induced myocardial inflammation by changing the balance of pro- and anti-inflammatory cytokines, inhibiting myeloperoxidase, an indicator of neutrophil activity, and inhibiting nitric oxide-mediated protein damage. DESIGN: Rats were randomized to treatment with either hypothermia (n = 6; 18 to 24 degrees C) or normothermia (n = 6; 36 to 38 degrees C). Endotoxin (15 mg/kg) was administered intravascularly to anesthetized animals, and heart tissue was harvested 150 min later. MEASUREMENTS AND RESULTS: Using enzyme-linked immunosorbent assays (ELISAs), we found that hypothermia induced myocardial expression of the anti-inflammatory cytokines interleukin (IL)-4 and IL-10, while decreasing concentrations of the pro-inflammatory cytokines IL-1beta and growth-related oncogene/cytokine-induced neutrophil chemoattractant (rat homolog of IL-8). Electromobility shift assay revealed that hypothermia inhibited the nuclear translocation of nuclear factor-kappaB. Reverse transcriptase-polymerase chain reaction and Western blot assays revealed that hypothermia attenuated the endotoxin-induced overexpression of both inducible nitric oxide synthase (iNOS) messenger RNA and iNOS protein, respectively. Hypothermia also attenuated nitric oxide-mediated myocardial protein damage, as determined by a nitrotyrosine ELISA. Myocardial myeloperoxidase content, an indicator of neutrophil accumulation and oxidative activity, was also inhibited by hypothermia in endotoxemic rats. CONCLUSION: These data demonstrate that hypothermia induces an anti-inflammatory cytokine profile, inhibits neutrophil aggregation, and inhibits the formation of nitric oxide during endotoxemia in the rat.


Subject(s)
Cytokines/metabolism , Endotoxemia/metabolism , Endotoxemia/therapy , Hypothermia, Induced , Myocardium/metabolism , Nitric Oxide/biosynthesis , Peroxidase/antagonists & inhibitors , Animals , Enzyme-Linked Immunosorbent Assay , Inflammation/prevention & control , Interleukin-1/metabolism , Interleukin-10/metabolism , Interleukin-4/metabolism , Interleukin-8/metabolism , Male , NF-kappa B/metabolism , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Random Allocation , Rats , Rats, Sprague-Dawley
4.
Shock ; 20(1): 41-5, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12813367

ABSTRACT

We recently reported that hypothermia protects against intrapulmonary nitric oxide overproduction and nitric oxide-mediated lung injury in endotoxemic rats. Few studies have been performed to investigate whether hypothermia reduces inflammation by affecting favorable changes in chemokine and pro- and anti-inflammatory cytokine profiles. In this study, we tested the hypothesis that hypothermia decreases concentrations of growth-related oncogene/cytokine-induced neutrophil chemoattractant-1 (GRO/CINC-1), interleukin (IL)-1beta, IL-6, and myeloperoxidase and increases concentration of IL-10 in the lungs endotoxemic rats. Twelve rats were anesthetized and randomized to treatment with either hypothermia (T = 18-24 degrees C; n = 6) or normothermia (T = 36-38 degrees C, n = 6). Endotoxin (15 mg/kg of Escherichia coli lipopolysaccharide) was administered intravascularly and lung tissue was harvested 150 min later. Three additional rats were sham instrumented and maintained as normothermic but not given endotoxin. Hematoxylin & eosin staining was performed for qualitative inspection of tissues. Quantitative analyses of lung homogenates were performed using enzyme-linked immunosorbent assays for IL-1beta, IL-6, IL-10, and GRO/CINC-1. Myeloperoxidase concentrations were determined using a colorimetric assay. Hypothermia attenuated the induction of intrapulmonary IL-1beta (P < 0.05), IL-6 (P < 0.05), GRO/CINC-1 (P < 0.05), and myeloperoxidase (P < 0.05) caused by endotoxin. Inspection of the lungs revealed that hypothermia similarly attenuated histological signs of injury, such as interstitial edema and neutrophil accumulation. Hypothermia increased the intrapulmonary concentration of IL-10 more than 3-fold over that measured in the normothermia (endotoxin-exposed) group (P < 0.05). Hypothermia inhibits neutrophil recruitment in the lungs of endotoxemic rats in part by decreasing proinflammatory cytokine expression. Additionally, hypothermia induces intrapulmonary IL-10 expression. Further studies are needed to investigate whether IL-10 mediates the anti-inflammatory effects of hypothermia.


Subject(s)
Chemokines, CXC , Endotoxemia/physiopathology , Endotoxemia/therapy , Hypothermia, Induced , Interleukin-10/metabolism , Lung/pathology , Animals , Blood Pressure , Chemokine CXCL1 , Chemokines/metabolism , Chemotactic Factors/metabolism , Disease Models, Animal , Endotoxemia/metabolism , Heart Rate , Hypothermia, Induced/adverse effects , Intercellular Signaling Peptides and Proteins/metabolism , Interleukin-1/metabolism , Interleukin-6/metabolism , Lung/metabolism , Lung Injury , Male , Peroxidase/metabolism , Rats , Rats, Sprague-Dawley
5.
Am J Physiol Lung Cell Mol Physiol ; 285(2): L484-91, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12716655

ABSTRACT

The in vivo mechanisms by which glucocorticoids inhibit nitric oxide expression await detailed investigation. In cell culture experiments, glucocorticoids have been shown to inhibit inducible nitric oxide synthase (iNOS) formation and activity. Glucocorticoids can inhibit iNOS activity in cultured cells by blocking arginine transport and inhibiting tetrahydrobiopterin biosynthesis. We recently reported that changes in intrapulmonary formation of nitric oxide in endotoxemic rats correspond with changes in transcription of the predominant arginine transporter cationic amino acid transporter (CAT)-2. Realizing that hemorrhagic shock induces nitric oxide overproduction in intact animals, we sought to explore whether glucocorticoids attenuate hemorrhagic shock-induced increases in intrapulmonary nitric oxide formation and whether they might do so by inhibiting the formation of tetrahydrobiopterin, iNOS protein, and CAT-2. We randomly assigned 10 male Sprague-Dawley rats to receive dexamethasone or normal saline. Bleeding the animals to a mean systemic blood pressure of between 40 and 45 mmHg created the hemorrhagic shock. Dexamethasone abrogated the increase in exhaled nitric oxide concentrations caused by hemorrhagic shock. At the end of the experiment, plasma nitrate/nitrite values were lower in the dexamethasone group than in the control group. The iNOS protein concentrations were also lower in the dexamethasone group than in the control group. Dexamethasone decreased the intrapulmonary iNOS mRNA concentrations yet increased both guanosine triphosphate cyclohydrolase I mRNA and CAT-2 mRNA. Our results support the idea that dexamethasone inhibits nitric oxide formation in a manner that is independent of tetrahydrobiopterin and arginine transport yet dependent on downregulation of iNOS mRNA expression.


Subject(s)
Cationic Amino Acid Transporter 2/genetics , Dexamethasone/pharmacology , GTP Cyclohydrolase/genetics , Gene Expression Regulation/drug effects , Lung/enzymology , Nitric Oxide Synthase/metabolism , RNA, Messenger/genetics , Administration, Inhalation , Animals , Base Sequence , Blood Pressure/drug effects , DNA Primers , Hemodynamics/drug effects , Hemodynamics/physiology , Lung/drug effects , Male , Nitrates/metabolism , Nitric Oxide/administration & dosage , Nitric Oxide/pharmacology , Nitric Oxide Synthase Type II , Nitrites/metabolism , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction , Shock, Hemorrhagic/genetics , Transcription, Genetic/drug effects
6.
Am J Physiol Lung Cell Mol Physiol ; 283(6): L1231-8, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12388361

ABSTRACT

Endotoxemia stimulates endogenous nitric oxide formation, induces transcription of arginine transporters, and causes lung injury. Hypothermia inhibits nitric oxide formation and is used as a means of organ preservation. We hypothesized that hypothermia inhibits endotoxin-induced intrapulmonary nitric oxide formation and that this inhibition is associated with attenuated transcription of enzymes that regulate nitric oxide formation, such as inducible nitric oxide synthase (iNOS) and the cationic amino acid transporters 1 (CAT-1) and 2 (CAT-2). Rats were anesthetized and randomized to treatment with hypothermia (18-24 degrees C) or normothermia (36-38 degrees C). Endotoxin was administered intravascularly. Concentrations of iNOS, CAT-1, CAT-2 mRNA, iNOS protein, and nitrosylated proteins were measured in lung tissue homogenates. We found that hypothermia abrogated the endotoxin-induced increase in exhaled nitric oxide and lung tissue nitrotyrosine concentrations. Western blot analyses revealed that hypothermia inhibited iNOS, but not endothelial nitric oxide synthase, protein expression in lung tissues. CAT-1, CAT-2, and iNOS mRNA concentrations were lower in the lungs of hypothermic animals. These findings suggest that hypothermia protects against intrapulmonary nitric oxide overproduction and nitric oxide-mediated lung injury by inhibiting transcription of iNOS, CAT-1, and CAT-2.


Subject(s)
Cationic Amino Acid Transporter 1/metabolism , Cationic Amino Acid Transporter 2/metabolism , Endotoxemia/metabolism , Hypothermia/metabolism , Lung/metabolism , Nitric Oxide Synthase/metabolism , Nitric Oxide/metabolism , Tyrosine/analogs & derivatives , Animals , Computer Systems , Immunoblotting , Male , Nitric Oxide Synthase Type II , Osmolar Concentration , Rats , Rats, Sprague-Dawley , Respiration , Reverse Transcriptase Polymerase Chain Reaction , Tyrosine/metabolism
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