Influence of the static magnetic field on red blood cells parameters and platelets using tests of CBC and microscopy images.

The present study was undertaken to investigate the influence of the static magnetic field (SMF) on the platelets and red blood cells counts (RBCs), haemoglobin (HGB), haematocrit (HCT), mean cell volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) in female rats (in-vitro). The results were analysed using tests of complete blood count (CBC) and microscopic images. The SMF in millitesla (mT) was generated using a fabricated electromagnetic exposure technique, and it has used for different time (minutes) of exposure. Statistical analysis for both tests investigated that the values of SMF affected the RBCs parameters and platelets for exposed Rats significantly for both tests. At 42.5 mT: RBCs counts have been affected (decreased) significantly (p < 0.05) at 30 min exposure, HCT (%) has affected (increased) significantly (p < 0.05) at 10 min and 30 min of exposure. The highest effects of SMF on the values of RBCs, HGB and HCT (%) were found at 34.8 mT after exposed to 15 min Variation of time of exposure and the values of SMF affected (p < 0.05) on the values of RBCs, HGB and HCT (%). The lowest effect of SMF was on the value of MCV (fl) at 6.4 mT at 15 min We found the effects of SMF on the platelet counts significant. Microscopy images of RBCs parameters and platelet count (PLT) support results got from the CBC test.

Effects of dietary synbiotic on innate immunity, antioxidant activity and disease resistance of Cirrhinus mrigala juveniles.

The dietary supplementation of synbiotic in Cirrhinus mrigala juvenile (with initial body weight ranging from 2.87 ±â€¯0.01 g to 3.26 ±â€¯0.05 g) was evaluated in terms of changes in innate immunity, antioxidant activity and disease resistance against Aeromonas hydrophilla infection. One hundred eighty acclimatized juveniles of mrigal were randomly distributed in the three replicates of each of four experimental groups i.e. control (without Probiotic and Prebiotic), T1 (High Probiotic + Low Prebiotic), T2 (Low Probiotic + High Prebiotic) and T3 (High Probiotic + High Prebiotic), using completely randomized design (CRD). At the end of the feeding trial for 60 days, fish were challenged by Aeromonas hydrophila and survival rate was recorded for the next 15 days. Bacillus subtilis used as a probiotic source and MOS used as a prebiotic source in the experiment. Results showed that innate immunity was comparatively improved in T3 group. Lysozyme activity and respiratory burst activity (NBT) were significantly (P < 0.05) affected in T3 group. Highest activities of antioxidant enzymes (P < 0.05) were reported in T3 group. Cumulative mortality % was found to be lower in the fish fed dietary synbiotic on T3 group after challenging with Aeromonas hydrophilla infection. The results of this study showed that under the experimental conditions, dietary supplementation of synbiotic had a synergestic effect on enhancing innate immunity and disease resistance of Cirrhinus mrigala (P < 0.05).

BEM46 shows eisosomal localization and association with tryptophan-derived auxin pathway in Neurospora crassa.

BEM46 proteins are evolutionarily conserved, but their functions remain elusive. We reported previously that the BEM46 protein in Neurospora crassa is targeted to the endoplasmic reticulum (ER) and is essential for ascospore germination. In the present study, we established a bem46 knockout strain of N. crassa. This Δbem46 mutant exhibited a level of ascospore germination lower than that of the wild type but much higher than those of the previously characterized bem46-overexpressing and RNA interference (RNAi) lines. Reinvestigation of the RNAi transformants revealed two types of alternatively spliced bem46 mRNA; expression of either type led to a loss of ascospore germination. Our results indicated that the phenotype was not due to bem46 mRNA downregulation or loss but was caused by the alternatively spliced mRNAs and the peptides they encoded. Using the N. crassa ortholog of the eisosomal protein PILA from Aspergillus nidulans, we further demonstrated the colocalization of BEM46 with eisosomes. Employing the yeast two-hybrid system, we identified a single interaction partner: anthranilate synthase component II (encoded by trp-1). This interaction was confirmed in vivo by a split-YFP (yellow fluorescent protein) approach. The Δtrp-1 mutant showed reduced ascospore germination and increased indole production, and we used bioinformatic tools to identify a putative auxin biosynthetic pathway. The genes involved exhibited various levels of transcriptional regulation in the different bem46 transformant and mutant strains. We also investigated the indole production of the strains in different developmental stages. Our findings suggested that the regulation of indole biosynthesis genes was influenced by bem46 overexpression. Furthermore, we uncovered evidence of colocalization of BEM46 with the neutral amino acid transporter MTR.