[The nature of metabolic processes in human dental hard tissues depending on their morphological structure]. / Kharakter obmennykh protsessov v tverdykh tkanyakh zubov cheloveka v zavisimosti ot ikh morfologicheskogo stroeniya.

THE STUDY OBJECTIVE: Was to give a comparative assessment of the movement of enamel and dentin fluid depending on the morphological structure of different sections of human dental hard tissues. MATERIALS AND METHODS: The research involved 140 female subjects aged 21-30 years. Sizes, form, surface of enamel-dentin junction, enamel prisms and their crystals in superficial, middle and deep sections, width, tortuosity, facing of dentin tubules of tested teeth were analyzed by scanning electron microscope Jeol JCM-5700 and atomic force microscope NTEGRA Prima. The biochemical study examined the quantitative content of water in the samples of enamel, dentin, enamel-dentin junction by weighing sample weights before and after drying. The data obtained were processed by methods of variation statistics using standard packages Microsoft Excel 2008, Statistica 12.0. RESULTS: The enamel-dentine junction of large-fastened type is characterized by zones of dentin integration into enamel at the distance of more than 10 mm, with regular arrangement of enamel prisms and wide dentine tubules of 3.55 to 4.70 µm in diameter; no water content reduction was found. The small-fastened type has less pronounced spots of dentin interference with enamel from 2 to 10 µm, the dentinal tubules are slightly curved with a diameter of 2.90 to 3.60 µm, the water content in dentin is reduced. In microfastened type, a linear structure with interpenetration zones up to 2 µm, enamel prisms slightly ordered, dentin tubules narrowed and curved in diameter from 1.30 to 1.95 µm, water content in enamel and dentin is reduced. CONCLUSION: At microfastened type of enamel-dentin junction, chaotic arrangement of enamel prisms and narrow dentin tubules water movement is broken and metabolic processes are reduced that determines high probability of exposure of dental hard tissue diseases.

[Determination of the quantitative content of lipids in a biological material by the method of IR spectroscopy.]

A modification of the Folch method has been proposed for the quantitative determination of lipids in biological material, in which, after extraction of lipids with a chloroform / ethanol mixture, lipids are determined using IR spectroscopy. The bands corresponding to the stretching and deformation vibrations of the methyl and methylene groups of lipids and fatty acids were selected as analytical absorption bands: 1396, 1458, 2853, and 2923 cm- 1. These bands do not intersect with the absorption bands of proteins ad nucleic acids, thus avoiding the stage of preliminary cleaning of lipids from non-lipid impurities. A multifactor regression model was constructed, which allows experimental data to be described with an error not exceeding 12%.

[Correlation interrelations between the composition of saliva and blood plasmain norm.]

Recently, the attention of researchers to the study of the properties of human saliva as a material with unique properties and diagnostic capabilities has increased. The purpose of the study: the study of the relationship between the biochemical composition of saliva and blood plasma is normal. 107 volunteers participated in the study, including 46 women (age 37.2 ± 3.9 years) and 61 men (age 36.1 ± 2.8 years). In all samples of saliva and blood plasma, 23 biochemical parameters were determined, including mineral and protein composition, enzyme activity, as well as indices of endogenous intoxication and lipid peroxidation. Nonparametric statistical methods are used to process the data obtained. It is shown that it is difficult to establish a unique relationship between the biochemical parameters of saliva and blood plasma. The calculation of the Spearman correlation coefficients has shown that only 7 of the 23 parameters demonstrate a weak correlation between the content in saliva and plasma. In general, the definition of saliva can have an independent diagnostic value, in this case, the parallel with the composition of serum and plasma is not appropriate. Nevertheless, the use of saliva in clinical laboratory diagnostics is associated with the need to establish criteria for the norm and pathology for each biochemical parameter.

[The circadian dynamics of composition of human saliva according data of infrared-Fourier-spectroscopy.]

The article demonstrates that infrared spectroscopy technique can be applied for detecting circadian changes of content of biological fluids, including saliva. The intensity of absorption bands in area of 1075 cm-1 can be used for evaluating both mineralization potential and defense characteristics of saliva glycoproteins. The absorption band of phospholipids correlates with content of calcium. The absorption bands 2963 and 3287 cm-1 characterize dynamics of coefficient Na/K that in turn provides information about activity of simpatico-adrenal system and thyroidal status. The existence of inner patterns in infrared spectrum permits to suppose that in normal state there is a balance between particular functional groups and any deviation can characterize various pathological states in human organism.