Mineralization and microbial utilization of poly(lactic acid) microplastic in soil.

The current carbon dioxide (CO2) evolution-based standard method for determining biodegradable microplastics (MPs) degradation neglects its priming effect on soil organic matter decomposition, which misestimates their biodegradability. Here, a 13C natural abundance method was used to estimate the mineralization of poly(lactic acid) (PLA) MP in various agricultural soils, and to trace its utilization in different microbial groups. In alkaline soils, the PLA-derived CO2 emissions increased with increasing soil carbon/nitrogen (C/N) ratios, and the mineralization of PLA MP concentrations ranged from 3-33 %, whereas the CO2 evolution method probably over- or under-estimated the mineralization of PLA in alkaline soils with different soil C/N ratios. Low PLA mineralization (1-5 %) were found in the acidic soil, and the standard method largely overestimated the mineralization of PLA MP by 1.3- to 3.3-fold. Moreover, the hydrolysate of PLA MP was preferentially assimilated by Gram-negative bacteria, but Gram-positive bacterial decomposition mainly contributed to the release of PLA-derived CO2 at low MP concentrations (≤ 1 %). Overall, the 13C natural abundance method appears to be suitable for tracking the mineralization and microbial utilization of biodegradable PLA in soils, and the PLA-derived C is mainly assimilated and decomposed by bacterial groups.

Effects of microplastics on soil microorganisms and microbial functions in nutrients and carbon cycling - A review.

The harmful effects of microplastics (MPs) pollution in the soil ecosystem have drawn global attention in recent years. This paper critically reviews the effects of MPs on soil microbial diversity and functions in relation to nutrients and carbon cycling. Reports suggested that both plastisphere (MP-microbe consortium) and MP-contaminated soils had distinct and lower microbial diversity than that of non-contaminated soils. Alteration in soil physicochemical properties and microbial interactions within the plastisphere facilitated the enrichment of plastic-degrading microorganisms, including those involved in carbon (C) and nutrient cycling. MPs conferred a significant increase in the relative abundance of soil nitrogen (N)-fixing and phosphorus (P)-solubilizing bacteria, while decreased the abundance of soil nitrifiers and ammonia oxidisers. Depending on soil types, MPs increased bioavailable N and P contents and nitrous oxide emission in some instances. Furthermore, MPs regulated soil microbial functional activities owing to the combined toxicity of organic and inorganic contaminants derived from MPs and contaminants frequently encountered in the soil environment. However, a thorough understanding of the interactions among soil microorganisms, MPs and other contaminants still needs to develop. Since currently available reports are mostly based on short-term laboratory experiments, field investigations are needed to assess the long-term impact of MPs (at environmentally relevant concentration) on soil microorganisms and their functions under different soil types and agro-climatic conditions.

Ecological and human health risk assessment of pharmaceutical compounds in the Sirsa River of Indian Himalayas.

The Baddi-Barotiwala-Nalagarh (BBN) region of Indian Himalayas is one of the most important pharmaceutical industrial clusters in Asia. This study investigated the distribution, and ecological and human health risks of four most frequently used pharmaceuticals [ciprofloxacin (CIP), norfloxacin (NOR), cetirizine (CTZ) and citalopram oxalate (ECP)] when co-occurring with metal ions in the Sirsa river water of the BBN region. The concentration range of the selected pharmaceuticals was between 'not detected' to 50 µgL-1 with some exception for CIP (50-100 µgL-1) and CTZ (100-150 µgL-1) in locations directly receiving wastewater discharges. A significant correlation was found between the occurrences of NOR and Al (r2 = 0.65; p = 0.01), and CTZ and K (r2 = 0.50; p = 0.01) and Mg (r2 = 0.50; p = 0.01). A high-level ecological risk [risk quotient (RQ) > 1] was observed for algae from all the pharmaceuticals. A medium-level risk (RQ = 0.01-0.1) was observed for Daphnia from CIP, NOR and ECP, and a high-level risk from CTZ. A low-level risk was observed for fishes from CIP and NOR, whereas CTZ and ECP posed a high-level risk to fishes. The overall risk to ecological receptors was in the order: CTZ > CIP > ECP > NOR. Samples from the river locations receiving water from municipal drains or situated near landfill and pharmaceutical factories exhibited RQ > 1 for all pharmaceuticals. The average hazard quotient (HQ) values for the compounds followed the order: CTZ (0.18) > ECP (0.15) > NOR (0.001) > CIP (0.0003) for children (0-6 years); ECP (0.49) > CTZ (0.29) > NOR (0.005) > CIP (0.001) for children (7-17 years), and ECP (0.34) > CTZ (0.21) > NOR (0.007) > CIP (0.001) for adults (>17 years). The calculated risk values did not readily confirm the status of water as safe or unsafe because the values of predicted no-effect concentration (PNEC) would depend on various other environmental factors such as quality of the toxicity data, and species sensitivity and distribution, which warrants further research.

Pristine and Fe-functionalized biochar for the simultaneous immobilization of arsenic and antimony in a contaminated mining soil.

This study examined the effectiveness of pristine biochar (BC) and Fe-functionalized biochar (FBC) in remediating As-Sb co-contaminated soil, and revealed the resulting impact on soil enzymatic activities and bacterial communities. Results from incubation experiments showed that the 1.5% FBC treatment reduced the bioavailable As and Sb concentration by 13.5% and 27.1%, respectively, in compared to the control, and reduced the proportion of specifically adsorbed and amorphous Fe-Mn oxide-bound metal(loid) fractions in the treated soil. Among the BC treatments, only the 1.5% BC treatment resulted in a reduction of bioavailable As by 11.7% and Sb by 21.4%. The 0.5% BC treatment showed no significant difference. The FBC achieved high As/Sb immobilization efficiency through Fe-induced electrostatic attraction, π-π electron donor-acceptor coordination, and complexation (Fe-O(H)-As/Sb) mechanisms. Additionally, the 1.5% FBC treatment led to a 108.2% and 367.4% increase in the activities of N-acetyl-ß-glucosaminidase and urease in soils, respectively, compared to the control. Furthermore, it significantly increased the abundance of Proteobacteria (15.2%), Actinobacteriota (37.0%), Chloroflexi (21.4%), and Gemmatimonadota (43.6%) at the phylum level. Co-occurrence network analysis showed that FBC was better than BC in increasing the complexity of bacterial communities. Partial least squares path modeling further indicated that the addition of biochar treatments can affect soil enzyme activities by altering soil bacterial composition. This study suggests that FBC application offers advantages in simultaneous As and Sb immobilization and restructuring the bacterial community composition in metal(loid)-contaminated soil.

Effect of particle size of nanoscale zero-valent copper on inorganic phosphorus adsorption-desorption in a volcanic ash soil.

Zero-valent copper engineered nanoparticles (Cu-ENPs) released through unintentional or intentional actions into the agricultural soils can alter the availability of inorganic phosphorus (IP) to plants. In this study, we used adsorption-desorption experiments to evaluate the effect of particle size of 1% Cu-ENPs (25 nm and 40-60 nm) on IP availability in Santa Barbara (SB) volcanic ash soil. X-Ray Diffraction results showed that Cu-ENPs were formed by a mixture of Cu metallic and Cu oxides (Cu2O or/and CuO) species, while specific surface area values showed that Cu-ENPs/25 nm could form larger aggregate particles compared to Cu-ENPs/40-60 nm. The kinetic IP adsorption of SB soil without and with 1% Cu-ENPs (25 nm and 40-60 nm) followed the mechanism described by the pseudo-second-order (k2 = 0.45-1.13 x 10-3 kg mmol-1 min-1; r2 ≥ 0.999, and RSS ≤ 0.091) and Elovich (α = 14621.10-3136.20 mmol kg-1 min-1; r2 ≥ 0.984, and RSS ≤ 69) models. Thus, the rate-limiting step for IP adsorption in the studied systems was chemisorption on a heterogeneous surface. Adsorption equilibrium isotherms without Cu-ENPs were fitted well to the Freundlich model, while with 1% Cu-ENPs (25 nm and 40-60 nm), isotherms were described best by the Freundlich and/or Langmuir model. The IP relative adsorption capacity (KF) was higher with 1% Cu-ENPs/40-60 nm (KF = 110.41) than for 1% Cu-ENPs/25 nm (KF = 74.40) and for SB soil (KF = 48.17). This study showed that plausible IP retention mechanisms in the presence of 1% Cu-ENPs in SB soil were: i) ligand exchange, ii) electrostatic attraction, and iii) co-precipitate formation. The desorption study demonstrated that 1% Cu-ENPs/40-60 nm increased the affinity of IP in SB soil with a greater effect than 1% Cu-ENPs/25 nm. Thus, both the studied size ranges of Cu-ENPs could favor an accumulation of IP in volcanic ash soils.

A comprehensive and global evaluation of residual antibiotics in agricultural soils: Accumulation, potential ecological risks, and attenuation strategies.

The occurrence of antibiotics in agricultural soils has raised concerns due to their potential risks to ecosystems and human health. However, a comprehensive understanding of antibiotic accumulation, distribution, and potential risks to terrestrial ecosystems on a global scale is still limited. Therefore, in this study, we evaluated the accumulation of antibiotics and their potential risks to soil microorganisms and plants, and highlighted the driving factors of antibiotic accumulation in agricultural soils based on 134 peer-reviewed studies (between 2000 and 2022). The results indicated that 56 types of antibiotics were detected at least once in agricultural soils with concentrations ranging from undetectable to over 7000 µg/kg. Doxycycline, tylosin, sulfamethoxazole, and enrofloxacin, belonging to the tetracyclines, macrolides, sulfonamides, and fluoroquinolones, respectively, were the most accumulated antibiotics in agricultural soil. The accumulation of TCs, SAs, and FQs was found to pose greater risks to soil microorganisms (average at 29.3%, 15.4%, and 21.8%) and plants (42.4%, 26.0%, and 38.7%) than other antibiotics. East China was identified as a hot spot for antibiotic contamination due to high levels of antibiotic concentration and ecological risk to soil microorganisms and plants. Antibiotic accumulation was found to be higher in vegetable fields (245.5 µg/kg) and orchards (212.4 µg/kg) compared to croplands (137.2 µg/kg). Furthermore, direct land application of manure resulted in a greater accumulation of TCs, SAs, and FQs accumulation in soils than compost fertilization. The level of antibiotics decreased with increasing soil pH and organic matter content, attributed to decreasing adsorption and enhancing degradation of antibiotics. In conclusion, this study highlights the need for further research on the impacts of antibiotics on soil ecological function in agricultural fields and their interaction mechanisms. Additionally, a whole-chain approach, consisting of antibiotic consumption reduction, manure management strategies, and remediation technology for soil contaminated with antibiotics, is needed to eliminate the potential environmental risks of antibiotics for sustainable and green agriculture.

Chitin and crawfish shell biochar composite decreased heavy metal bioavailability and shifted rhizosphere bacterial community in an arsenic/lead co-contaminated soil.

Sustainable management of ever-increasing organic biowaste and arable soil contamination by potentially toxic elements are of concern from both environmental and agricultural perspectives. To tackle the waste issue of crawfish shells and simultaneously minimize the threat of arsenic (As) and lead (Pb) to human health, a pot trial was conducted using chitin (CT), crawfish shell biochar (CSB), crawfish shell powder (CSP), and CT-CSB composite to compare their remediation efficiencies in As/Pb co-contaminated soil. Results demonstrated that addition of all amendments decreased Pb bioavailability, with the greatest effect observed for the CT-CSB treatment. Application of CSP and CSB increased the soil available As concentration, while significant decreases were observed in the CT and CT-CSB treatments. Meanwhile, CT addition was the most effective in enhancing the soil enzyme activities including acid phosphatase, α-glucosidase, N-acetyl-ß-glucosaminidase, and cellobiohydrolase, whereas CSB-containing treatments suppressed the activities of most enzymes. The amendments altered the bacterial abundance and composition in soil. For instance, compared to the control, all treatments increased Chitinophagaceae abundance by 2.6-4.7%. The relative abundance of Comamonadaceae decreased by 1.6% in the CSB treatment, while 2.1% increase of Comamonadaceae was noted in the CT-CSB treatment. Redundancy and correlation analyses (at the family level) indicated that the changes in bacterial community structure were linked to bulk density, water content, and As/Pb availability of soils. Partial least squares path modeling further indicated that soil chemical property (i.e., pH, dissolved organic carbon, and cation exchange capacity) was the strongest predictor of As/Pb availability in soils following amendment application. Overall, CT-CSB could be a potentially effective amendment for simultaneously immobilizing As and Pb and restoring soil ecological functions in contaminated arable soils.

Effect of surfactants on the sorption-desorption, degradation, and transport of chlorothalonil and hydroxy-chlorothalonil in agricultural soils.

The fungicide chlorothalonil (CTL) and its metabolite hydroxy chlorothalonil (OH-CTL) constitute a risk of soil and water contamination, highlighting the need to find suitable soil remediation methods for these compounds. Surfactants can promote the bioavailability of organic compounds for enhanced microbial degradation, but the performance depends on soil and surfactant properties, sorption-desorption equilibria of contaminants and surfactants, and possible adverse effects of surfactants on microorganisms. This study investigated the influence of five surfactants [e.g., Triton X-100 (TX-100), sodium dodecyl sulphate (SDS), hexadecyltrimethylammonium bromide (HDTMA), Aerosol 22 and Tween 80] on the sorption-desorption, degradation, and mobility of CTL and OH-CTL in two volcanic and one non-volcanic soil. Sorption and desorption of fungicides depended on the sorption of surfactants on soils, surfactants' capacity to neutralize the net negative charge of soils, surfactants' critical micellar concentration, and pH of soils. HDTMA was strongly adsorbed on soils, which shifted the fungicide sorption equilibria by increasing the distribution coefficient (Kd) values. Contrarily, SDS and TX-100 lowered CTL and OH-CTL sorption on soils by decreasing the Kd values, which resulted in an efficient extraction of the fungicide compounds from soil. SDS increased the degradation of CTL, especially in the non-volcanic soil (DT50 values were 14 and 7 days in natural and amended soils, with final residues <7% of the initial dose), whereas TX-100 enabled an early start and sustenance of OH-CTL degradation in all soils. CTL and OH-CTL stimulated soil microbial activities without noticeable deleterious effects of the surfactants. SDS and TX-100 also reduced the vertical transport of OH-CTL in soils. Results of this study could be extended to soils in other regions of the world because the tested soils represent widely different physical, chemical, and biological properties.

Occurrence profiling and environmental risk assessment of veterinary antibiotics in vegetable soils at Chongqing region, China.

Veterinary antibiotics (VAs) are emerging contaminants in soils as they may pose high risks to the ecosystem and human health. Identifying VAs accumulation in soils is essential for assessing their potential risks. Therefore, we investigated the distribution of VAs in soils from vegetable fields and evaluated their potential ecological and antimicrobial resistance risks in the Chongqing region of the Three Gorges Reservoir area, China. Results indicated that twenty-six species of VAs, including nine sulfonamides (SAs), seven quinolones (QNs), four tetracyclines (TCs), four macrolides (MLs), and two other species of VAs were detected in soils, with their accumulative levels ranging from 1.4 to 3145.7 µg kg-1. TCs and QNs were the dominant VAs species in soils with high detection frequencies (100% TCs and 80.6% for QNs) and accumulative concentration (up to 1195 µg kg-1 for TCs and up to 485 µg kg-1 for QNs). Risk assessment indices showed that VAs (specifically SAs, TCs, and QNs) in most vegetable soils would pose a medium to high risk to the ecosystem and antimicrobial resistance. Mixture of VAs posed a higher risk to soil organisms, antimicrobial resistance, and plants than to aquatic organisms. Modeling analysis indicated that socioeconomic conditions, farmers' education levels, agricultural practices, and soil properties were the main factors governing VAs accumulation and environmental risks. Farmers with a high educational level owned large-scale farms and were more willing to use organic fertilizers for vegetable production, which eventually led to high VAs accumulation in vegetable soil. These findings would provide a reference for sustainable agricultural and environmental production under the current scenario of chemical fertilizer substitution by organic products and green agricultural development.

Enhancing cation and anion exchange capacity of rice straw biochar by chemical modification for increased plant nutrient retention.

Biochar, a potential alternative of infield crop residue burning, can prevent nutrient leaching from soil and augment soil fertility. However, pristine biochar contains low cation (CEC) and anion (AEC) exchange capacity. This study developed fourteen engineered biochar by treating a rice straw biochar (RBC-W) first separately with different CEC and AEC enhancing chemicals, and then with their combined treatments to increase CEC and AEC in the novel biochar composites. Following a screening experiment, promising engineered biochar, namely RBC-W treated with O3-HCl-FeCl3 (RBC-O-Cl), H2SO4-HNO3-HCl-FeCl3 (RBC-A-Cl), and NaOH-Fe(NO3)3(RBC-OH-Fe), underwent physicochemical characterization and soil leaching-cum nutrient retention studies. RBC-O-Cl, RBC-A-Cl, and RBC-OH-Fe recorded a spectacular rise in CEC and AEC over RBC-W. All the engineered biochar remarkably reduced the leaching of NH4+-N, NO3- -N, PO43--P and K+ from a sandy loam soil and increased retention of these nutrients. RBC-O-Cl at 4.46 g kg-1 dosage emerged as the most effective soil amendment increasing the retention of above ions by 33.7, 27.8, 15.0, and 5.74 % over a comparable dose of RBC-W. The engineered biochar could thus enhance plants' nutrient use efficiency and reduce the use of costly chemical fertilizers that are harmful to environmental quality.

New insight into the mechanisms of preferential encapsulation of metal(loid)s by wheat phytoliths under silicon nanoparticle amendment.

Silicon nanoparticles (SiNPs) have been widely used to immobilize toxic trace metal(loid)s (TTMs) in contaminated croplands. However, the effect and mechanisms of SiNP application on TTM transportation in response to phytolith formation and phytolith-encapsulated-TTM (PhytTTM) production in plants are unclear. This study demonstrates the promotion effect of SiNP amendment on phytolith development and explores the associated mechanisms of TTM encapsulation in wheat phytoliths grown on multi-TTM contaminated soil. The bioconcentration factors between organic tissues and phytoliths of As and Cr (> 1) were significantly higher than those of Cd, Pb, Zn and Cu, and about 10 % and 40 % of the total As and Cr that bioaccumulated in wheat organic tissues were encapsulated into the corresponding phytoliths under high-level SiNP treatment. These observations demonstrate that the potential interaction of plant silica with TTMs is highly variable among elements, with As and Cr being the two most strongly concentrated TTMs in the phytoliths of wheat treated with SiNPs. The qualitative and semi-quantitative analyses of the phytoliths extracted from wheat tissues suggest that the high pore space and surface area (≈ 200 m2 g-1) of phytolith particles could have contributed to the embedding of TTMs during silica gel polymerization and concentration to form PhytTTMs. The abundant SiO functional groups and high silicate-minerals in phytoliths are dominant chemical mechanisms for the preferential encapsulation of TTMs (i.e., As and Cr) by wheat phytoliths. Notably, the organic carbon and bioavailable Si of soils and the translocation of minerals from soil to plant aerial parts can impact TTM sequestration by phytoliths. Thus, this study has implications for the distribution or detoxification of TTMs in plants via preferential PhytTTM production and biogeochemical cycling of PhytTTMs in contaminated cropland following exogenous Si supplementation.

Synthesis and characterization of PCN-222 metal organic framework and its application for removing perfluorooctane sulfonate from water.

Poly- and perfluoro alkyl substances (PFAS) are a group of man-made, notoriously persistent, and highly toxic contaminants in the environment reported worldwide. Many adsorbents including granular activated carbon, graphene, biochar, zeolites, and clay minerals have been tested for PFAS removal from water, but most of these materials suffer from high cost and/or poor removal performance. Here, we synthesized, characterized, and examined the efficiency of PCN-222(Fe), a new porous metal organic framework (MOF) with high water stability, for adsorptive removal of a frequently occurring PFAS, perfluorooctane sulfonate (PFOS), from water. The adsorption isotherm and kinetic studies revealed high PFOS adsorption capacity of PCN-222 (2257 mg/g), with rapid PFOS removal rate (within 30 min). The structure of PCN-222 was unaffected in water in the pH range of 2-10 but disintegrated and lost its PFOS removal ability at pH > 10. The PFOS adsorption on PCN-222 was an endothermic reaction. Electrostatic attraction was a dominant mechanism for PFOS adsorption at < 1694 mg/g PFOS concentration, while hydrophobic interaction accompanied with hydrogen-bonding was responsible at ≥ 1694 mg/g PFOS concentration. The interlayer morphology of PCN-222 did not change due to increasing PFOS loading. The findings of this study demonstrated superior features of PCN-222 over other conventional adsorbents for its potential application in removing PFOS from contaminated water to reduce PFOS transfer from water to living organisms.

A mechanistic insight into the shrinkage and swelling of Ca-montmorillonite upon adsorption of chain-like ranitidine in an aqueous system.

Adsorption behavior of ranitidine hydrochloride (RT) on a Ca-montmorillonite (SAz-1) was studied in aqueous system through batch experiments. The adsorption kinetics revealed that the equilibrium reached within 0.25 h and the data fitted well to the pseudo-second order kinetic equation (R2 = 0.98). The maximum RT adsorption capacity of SAz-1 was 369.2 mg/g and the adsorption isotherm data followed the Langmuir model (R2 = 0.99). The adsorption of RT and desorption of exchangeable cations from the clay mineral were linearly correlated, suggesting that cation exchange was the dominant mechanism of RT adsorption. The XRD examination of RT-adsorbed SAz-1 samples (unsaturated/saturated) after heating enabled the calculation of RT occupied area in the interlayer of the clay mineral. The results suggested that adsorbed-RT at low loading rate could lay on the internal surfaces in a free style to reduce the basal spacing (d001 value) of SAz-1. When the RT loading rate was increased, a limited surface space enforced more RT molecules to lay in a tilted style and caused interlayer swelling of SAz-1 increasing the d001 value. The trend of rising decomposition temperature of RT with increasing RT loading rates confirmed intercalation of RT molecules in SAz-1. Infrared spectral analysis revealed the participation of amide and furan groups of RT in binding between RT and SAz-1. Thus, this study indicated that SAz-1 is an efficient adsorbent to remove RT from contaminated water, and the chain-like molecular structure of RT could cause an irregular change in the basal spacing of swelling type clay minerals.

Environmental impact of metal halide perovskite solar cells and potential mitigation strategies: A critical review.

Metal halide perovskite solar cells (PSCs) have gained extensive attention in the field of solar photovoltaic technology over the past few years. Despite being a remarkable alternative to fossil fuels, solar cells may have detrimental effects on the environment and human health owing to the use of toxic materials during manufacturing. Although modern metal-halide-based PSCs are stable and have encapsulation to prevent the release of potentially toxic materials into the environment, their destruction due to strong winds, hail, snow, landslides, fires, or waste disposal can result in the exposure of these materials to the environment. This may lead to the contamination of soil and groundwater, and uptake of potentially toxic elements by plants, subsequently affecting humans and other living organisms via food chain contamination. Despite worldwide concern, the environmental and ecotoxicological impacts of metal-halide-based PSCs have not been comprehensively surveyed. This review summarizes and critically evaluates the current status of metal-halide-based PSC production and its impact on environmental sustainability, food security, and human health. Furthermore, safe handling and disposal methods for the waste generated from metal-halide-based PSCs are proposed, with a focus on recycling and reuse. Although some studies have suggested that the amount of lead released from metal halide PSCs is far below the maximum permissible levels in most soils, a clear conclusion cannot be reached until real contamination scenarios are assessed under field conditions. Precautions must be taken to minimize environmental contamination throughout the lifecycle of PSCs until nontoxic and similarly performing alternative solar photovoltaic products are developed.

Plant-derived saponin enhances biodegradation of petroleum hydrocarbons in the rhizosphere of native wild plants.

Plant-derived saponins are bioactive surfactant compounds that can solubilize organic pollutants in environmental matrices, thereby facilitating pollutant remediation. Externally applied saponin has potential to enhance total petroleum hydrocarbon (TPH) biodegradation in the root zone (rhizosphere) of wild plants, but the associated mechanisms are not well understood. For the first time, this study evaluated a triterpenoid saponin (from red ash leaves, Alphitonia excelsa) in comparison to a synthetic surfactant (Triton X-100) for their effects on plant growth and biodegradation of TPH in the rhizosphere of two native wild species (a grass, Chloris truncata, and a shrub, Hakea prostrata). The addition of Triton X-100 at the highest level (1000 mg/kg) in the polluted soil significantly hindered the plant growth (reduced plant biomass and photosynthesis) and associated rhizosphere microbial activity in both the studied plants. Therefore, TPH removal in the rhizosphere of both plant species treated with the synthetic surfactant was not enhanced (at the lower level, 500 mg/kg soil) and even slightly decreased (at the highest level) compared to that in the surfactant-free (control) treatment. By contrast, TPH removal was significantly increased with saponin application (up to 60% in C. truncata at 1000 mg/kg due to enhanced plant growth and associated rhizosphere microbial activity). No significant difference was observed between the two saponin application levels. Dehydrogenase activity positively correlated with TPH removal (p < 0.001) and thus this parameter could be used as an indicator to predict the rhizoremediation efficiency. This work indicates that saponin-amended rhizoremediation could be an environmentally friendly and effective biological approach to remediate TPH-polluted soils. It was clear that the enhanced plant growth and rhizosphere microbial activity played a crucial role in TPH rhizoremediation efficiency. The saponin-induced molecular processes that promoted plant growth and soil microbial activity in the rhizosphere warrant further studies.

The effects of light regime on carbon cycling, nutrient removal, biomass yield, and polyhydroxybutyrate (PHB) production by a constructed photosynthetic consortium.

Microalgae can add value to biological wastewater treatment processes by capturing carbon and nutrients and producing valuable biomass. Harvesting small cells from liquid media is a challenge easily addressed with biofilm cultivation. Three experimental photobioreactors were constructed from inexpensive materials (e.g. plexiglass, silicone) for hybrid liquid/biofilm cultivation of a microalgal-bacterial consortia in aquaculture effluent. Three light regimes (full-spectrum, blue-white, and red) were implemented to test light spectra as a process control. High-intensity full-spectrum light caused photoinhibition and low biomass yield, but produced the most polyhydroxybutyrate (PHB) (0.14 mg g-1); a renewable bioplastic polymer. Medium-intensity blue-white light was less effective for carbon capture, but removed up to 82 % of phosphorus. Low-intensity red light was the only net carbon-negative regime, but increased phosphorus (+4.98 mg/L) in the culture medium. Light spectra and intensity have potential as easily-implemented process controls for targeted wastewater treatment, biomass production, and PHB synthesis using photosynthetic consortia.

Nitrogen-enriched biochar co-compost for the amelioration of degraded tropical soil.

Tropical soils are often deeply weathered and vulnerable to degradation having low pH and unfavorable Al/Fe levels, which can constrain crop production. This study aims to examine nitrogen-enriched novel biochar co-composts prepared from rice straw, maize stover, and gram residue in various mixing ratios of the biochar and their feedstock materials for the amelioration of acidic tropical soil. Three pristine biochar and six co-composts were prepared, characterized, and evaluated for improving the chemical and biological quality of the soil against a conventional lime treatment. The pH, cation exchange capacity (CEC), calcium carbonate equivalence (CCE) and nitrogen content of co-composts varied between 7.78-8.86, 25.3-30.5 cmol (p+) kg-1, 25.5-30.5%, and 0.81-1.05%, respectively. The co-compost prepared from gram residue biochar mixed with maize stover at a 1:7 dry-weight ratio showed the highest rise in soil pH and CEC, giving an identical performance with the lime treatment and significantly better effect (p < .05) than the unamended control. Agglomerates of calcite and dolomite in biochar co-composts, and surface functional groups contributed to pH neutralization and increased CEC of the amended soil. The co-composts also significantly (p < .05) increased the dehydrogenase (1.87 µg TPF g-1 soil h-1), ß-glucosidase (90 µg PNP g-1 soil h-1), and leucine amino peptidase (3.22 µmol MUC g-1 soil h-1) enzyme activities in the soil, thereby improving the soil's biological quality. The results of this study are encouraging for small-scale farmers in tropical developing countries to sustainably reutilize crop residues via biochar-based co-composting technology.

Designer biochar with enhanced functionality for efficient removal of radioactive cesium and strontium from water.

Radioactive elements released into the environment by accidental discharge constitute serious health hazards to humans and other organisms. In this study, three gasified biochars prepared from feedstock mixtures of wood, chicken manure, and food waste, and a KOH-activated biochar (40% food waste + 60% wood biochar (WFWK)) were used to remove cesium (Cs+) and strontium (Sr2+) ions from water. The physicochemical properties of the biochars before and after adsorbing Cs+ and Sr2+ were determined using X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, extended X-Ray absorption fine structure (EXAFS) spectroscopy, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX). The WFWK exhibited the highest adsorption capacity for Cs+ (62.7 mg/g) and Sr2+ (43.0 mg/g) among the biochars tested herein. The removal of radioactive 137Cs and 90Sr exceeded 80% and 47%, respectively, in the presence of competing ions like Na+ and Ca2+. The functional groups present in biochar, including -OH, -NH2, and -COOH, facilitated the adsorption of Cs+ and Sr2+. The Cs K-edge EXAFS spectra revealed that a single coordination shell was assigned to the Cs-O bonding at 3.11 Å, corresponding to an outer-sphere complex formed between Cs and the biochar. The designer biochar WFWK may be used as an effective adsorbent to treat radioactive 137Cs- and 90Sr-contaminated water generated during the operation of nuclear power plants and/or unintentional release, owing to the enrichment effect of the functional groups in biochar via alkaline activation.

Removal of nanoplastics in water treatment processes: A review.

Nanoplastics are drawing a significant attention as a result of their propensity to spread across the environment and pose a threat to all organisms. The presence of nanoplastics in water is given attention nowadays as the transit of nanoplastics occurs through the aquatic ecosphere besides terrestrial mobility. The principal removal procedures for macro-and micro-plastic particles are effective, but nanoparticles escape from the treatment, increasing in the water and significantly influencing the society. This critical review is aimed to bestow the removal technologies of nanoplastics from aquatic ecosystems, with a focus on the treatment of freshwater, drinking water, and wastewater, as well as the importance of transit and its impact on health concerns. Still, there exists a gap in providing a collective knowledge on the methods available for nanoplastics removal. Hence, this review offered various nanoplastic removal technologies (microorganism-based degradation, membrane separation with a reactor, and photocatalysis) that could be the practical/effective measures along with the traditional procedures (filtration, coagulation, centrifugation, flocculation, and gravity settling). From the analyses of different treatment systems, the effectiveness of nanoplastics removal depends on various factors, source, size, and type of nanoplastics apart from the treatment method adopted. Combined removal methods, filtration with coagulation offer great scope for the removal of nanoplastics from drinking water with >99 % efficiency. The collected data could serve as base-line information for future research and development in water nanoplastics cleanup.

Biochar-microorganism interactions for organic pollutant remediation: Challenges and perspectives.

Numerous harmful chemicals are introduced every year in the environment through anthropogenic and geological activities raising global concerns of their ecotoxicological effects and decontamination strategies. Biochar technology has been recognized as an important pillar for recycling of biomass, contributing to the carbon capture and bioenergy industries, and remediation of contaminated soil, sediments and water. This paper aims to critically review the application potential of biochar with a special focus on the synergistic and antagonistic effects on contaminant-degrading microorganisms in single and mixed-contaminated systems. Owing to the high specific surface area, porous structure, and compatible surface chemistry, biochar can support the proliferation and activity of contaminant-degrading microorganisms. A combination of biochar and microorganisms to remove a variety of contaminants has gained popularity in recent years alongside traditional chemical and physical remediation technologies. The microbial compatibility of biochar can be improved by optimizing the surface parameters so that toxic pollutant release is minimized, biofilm formation is encouraged, and microbial populations are enhanced. Biocompatible biochar thus shows potential in the bioremediation of organic contaminants by harboring microbial populations, releasing contaminant-degrading enzymes, and protecting beneficial microorganisms from immediate toxicity of surrounding contaminants. This review recommends that biochar-microorganism co-deployment holds a great potential for the removal of contaminants thereby reducing the risk of organic contaminants to human and environmental health.