ABSTRACT
Two different glucans (water-soluble PS-I, water-insoluble PS-II) were isolated from the alkaline extract of the fruit bodies of hybrid mushroom. PS-I was found to consist of only (1â6)-linked ß-D-glucopyranose. PS-II was composed of terminal, (1â3,4)-linked, and (1â3)-linked ß-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:1. PS-I showed macrophages, splenocytes, and thymocytes activation as well as antioxidant property. On the basis of sugar analysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these glucans were established as:
Subject(s)
Glucans/chemistry , Glucans/pharmacology , Hybridization, Genetic , Pleurotus/chemistry , Pleurotus/genetics , Volvariella/chemistry , Volvariella/genetics , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/isolation & purification , Adjuvants, Immunologic/pharmacology , Animals , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/pharmacology , Biphenyl Compounds/chemistry , Carbohydrate Sequence , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Glucans/isolation & purification , Hydrogen-Ion Concentration , Macrophages, Peritoneal/drug effects , Macrophages, Peritoneal/immunology , Mice , Molecular Sequence Data , Picrates/chemistry , Solubility , Spleen/cytology , Thymocytes/cytology , Thymocytes/drug effects , Water/chemistryABSTRACT
Three polysaccharide fractions (PS-I, PS-II, and PS-III) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-ß glucan. PS-II and PS-III were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as;
Subject(s)
Chimera/metabolism , Galactans/metabolism , Glucans/metabolism , Pleurotus , Volvariella , Carbohydrate Conformation , Carbohydrate Sequence , Chimera/genetics , Galactans/chemistry , Galactans/isolation & purification , Glucans/chemistry , Glucans/isolation & purification , Glucose/chemistry , Inbreeding , Magnetic Resonance Spectroscopy , Mannose/chemistry , Molecular Sequence DataABSTRACT
A water-soluble polysaccharide (PS-I), isolated from the aqueous extract of the stems of Amaranthus tricolor Linn. (Amaranthus gangeticus L.), was found to consist of L-arabinose, methyl-D-galacturonate, D-galactose, and 3-O-Ac-L-rhamnose in a molar ratio of nearly 1:1:1:1. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the PS-I is determined as: -->5)-alpha-L-Araf-(1-->4)-beta-D-Galp-(1-->2)-3-O-Ac-beta-L-Rhap-(1-->4)-alpha-D-Gal-A6Me-(1-->
Subject(s)
Amaranthus/chemistry , Polysaccharides/chemistry , Carbohydrate Sequence , Hot Temperature , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Plant Extracts/chemistry , Plant Stems/chemistry , Polysaccharides/isolation & purification , WaterABSTRACT
A water-soluble polysaccharide was isolated from hot aqueous extracts of fruits of Psidium guajava. The polysaccharide was found to contain 2-O-methyl-l-arabinose, 2-O-acetyl-D-galactose, and D-methyl galacturonate in a molar ratio of approximately 1:1:1. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as [carbohydrate structure: see text].
Subject(s)
Fruit/chemistry , Hot Temperature , Plant Extracts/chemistry , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Psidium/chemistry , Water/chemistry , Carbohydrate Sequence , Magnetic Resonance Spectroscopy , Molecular Sequence DataABSTRACT
A water-soluble polysaccharide, isolated from the hot aqueous extract of an edible mushroom, Agaricus bitorquis, was found to consist of d-glucose only. On the basis of total hydrolysis, methylation analysis, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit was established as -->6)-beta-D-Glcp-(1-->.
