Ageing leads to reduced specificity of antimicrobial peptide responses in Drosophila melanogaster.

Evolutionary theory predicts a late-life decline in the force of natural selection, possibly leading to late-life deregulations of the immune system. A potential outcome of such deregulations is the inability to produce specific immunity against target pathogens. We tested this possibility by infecting multiple Drosophila melanogaster lines (with bacterial pathogens) across age groups, where either individual or different combinations of Imd- and Toll-inducible antimicrobial peptides (AMPs) were deleted using CRISPR gene editing. We show a high degree of non-redundancy and pathogen-specificity of AMPs in young flies: in some cases, even a single AMP could confer complete resistance. However, ageing led to drastic reductions in such specificity to target pathogens, warranting the action of multiple AMPs across Imd and Toll pathways. Moreover, use of diverse AMPs either lacked survival benefits or even accompanied survival costs post-infection. These features were also sexually dimorphic: females required a larger repertoire of AMPs than males but extracted equivalent survival benefits. Finally, age-specific expansion of the AMP-repertoire was accompanied with ageing-induced downregulation of negative-regulators of the Imd pathway and damage to renal function post-infection, as features of poorly regulated immunity. Overall, we could highlight the potentially non-adaptive role of ageing in producing less-specific AMP responses, across sexes and pathogens.

Free-Fermionic Topological Quantum Sensors.

Second order quantum phase transitions, with well-known features such as long-range entanglement, symmetry breaking, and gap closing, exhibit quantum enhancement for sensing at criticality. However, it is unclear which of these features are responsible for this enhancement. To address this issue, we investigate phase transitions in free-fermionic topological systems that exhibit neither symmetry-breaking nor long-range entanglement. We analytically demonstrate that quantum enhanced sensing is possible using topological edge states near the phase boundary. Remarkably, such enhancement also endures for ground states of such models that are accessible in solid state experiments. We illustrate the results with 1D Su-Schrieffer-Heeger chain and a 2D Chern insulator which are both experimentally accessible. While neither symmetry-breaking nor long-range entanglement are essential, gap closing remains as the major candidate for the ultimate source of quantum enhanced sensing. In addition, we also provide a fixed and simple measurement strategy that achieves near-optimal precision for sensing using generic edge states irrespective of the parameter value. This paves the way for development of topological quantum sensors which are expected to also be robust against local perturbations.

Intergenerational paternal effect of adult density in Drosophila melanogaster.

Notwithstanding recent evidences, paternal environment is thought to be a potential but unlikely source of fitness variation that can affect trait evolution. Here we studied intergenerational effects of males' exposure to varying adult density in Drosophila melanogasterlaboratory populations.We held sires at normal (N), medium (M) and high (H) adult densities for 2 days before allowing them to mate with virgin females. This treatment did not introduce selection through differential mortality. Further, we randomly paired males and females and allowed a single round of mating between the sires and the dams. We then collected eggs from the dams and measured the egg size. Finally, we investigated the effect of the paternal treatment on juvenile and adult (male) fitness components.We found a significant treatment effect on juvenile competitive ability where the progeny sired by the H-males had higher competitive ability. Since we did not find the treatment to affect egg size, this effect is unlikely to be mediated through variation in female provisioning.Male fitness components were also found to have a significant treatment effect: M-sons had lower dry weight at eclosion, higher mating latency, and lower competitive mating success.While being the first study to show both adaptive and non-adaptive effect of the paternal density in Drosophila, our results highlight the importance of considering paternal environment as important source of fitness variation.