Trends in Biological Ammonia Production.

Food production heavily depends on ammonia-containing fertilizers to improve crop yield and profitability. However, ammonia production is challenged by huge energy demands and the release of ~2% of global CO2. To mitigate this challenge, many research efforts have been made to develop bioprocessing technologies to make biological ammonia. This review presents three different biological approaches that drive the biochemical mechanisms to convert nitrogen gas, bioresources, or waste to bio-ammonia. The use of advanced technologies-enzyme immobilization and microbial bioengineering-enhanced bio-ammonia production. This review also highlighted some challenges and research gaps that require researchers' attention for bio-ammonia to be industrially pragmatic.

Understanding gaseous reduction in swine manure resulting from nanoparticle treatments under anaerobic storage conditions.

Manure is an impending source of carbon (C), sulfur (S) and water (H2O). Consequently, microbial populations utilize these constituents to produce methane (CH4), carbon dioxide (CO2), greenhouse gases (GHGs), and hydrogen sulfide (H2S). Application of nanoparticles (NPs) to stored manure is an emerging GHG mitigation technique. In this study, two NPs: nano zinc oxide (nZnO) and nano silver (nAg) were tested in swine manure stored under anaerobic conditions to determine their effectiveness in mitigating gaseous emissions and total gas production. The biological sources of gas production, i.e., microbial populations were characterized via Quantitative Polymerase Chain Reaction (qPCR) analysis. Additionally, pH, redox, and VFAs were determined using standard methods. Each treatment of the experiment was replicated three times and NPs were applied at a dose of 3 g/L of manure. Also, headspace gas from all treatment replicates were analyzed for CH4 and CO2 gas concentrations using an SRI-8610 Gas Chromatograph and H2S concentrations were measured using a Jerome 631X meter. Nanoparticles tested in this study reduced the cumulative gas volume by 16%-79% compared to the control. Among the NPs tested, only nZnO consistently reduced GHG concentrations by 37%-97%. Reductions in H2S concentrations ranged from 87% to 97%. Gaseous reductions were likely due to decreases in the activity and numbers of specific gas producing methanogenic archaea and sulfate reducing bacterial (SRB) species.

Nanoparticles in mitigating gaseous emissions from liquid dairy manure stored under anaerobic condition.

A number of mitigation techniques exist to reduce the emissions of pollutant gases and greenhouse gases (GHGs) from anaerobic storage of livestock manure. Nanoparticle (NP) application is a promising mitigating treatment option for pollutant gases, but limited research is available on the mode of NP application and their effectiveness in gaseous emission reduction. In this study, zinc silica nanogel (ZnSNL), copper silica nanogel (CuSNL), and N-acetyl cysteine (NACL) coated zinc oxide quantum dot (Qdot) NPs were compared to a control lacking NPs. All three NPs tested significantly reduced gas production and concentrations compared to non-treated manure. Overall, cumulative gas volumes were reduced by 92.73%-95.83%, and concentrations reduced by 48.98%-99.75% for H2S, and 20.24%-99.82% for GHGs. Thus, application of NPs is a potential treatment option for mitigating pollutant and GHG emissions from anaerobically stored manure.

In vitro evaluation of nano zinc oxide (nZnO) on mitigation of gaseous emissions.

BACKGROUND: Enteric methane (CH4) accounts for about 70% of total CH4 emissions from the ruminant animals. Researchers are exploring ways to mitigate enteric CH4 emissions from ruminants. Recently, nano zinc oxide (nZnO) has shown potential in reducing CH4 and hydrogen sulfide (H2S) production from the liquid manure under anaerobic storage conditions. Four different levels of nZnO and two types of feed were mixed with rumen fluid to investigate the efficacy of nZnO in mitigating gaseous production. METHODS: All experiments with four replicates were conducted in batches in 250 mL glass bottles paired with the ANKOMRF wireless gas production monitoring system. Gas production was monitored continuously for 72 h at a constant temperature of 39 ± 1 °C in a water bath. Headspace gas samples were collected using gas-tight syringes from the Tedlar bags connected to the glass bottles and analyzed for greenhouse gases (CH4 and carbon dioxide-CO2) and H2S concentrations. CH4 and CO2 gas concentrations were analyzed using an SRI-8610 Gas Chromatograph and H2S concentrations were measured using a Jerome 631X meter. At the same time, substrate (i.e. mixed rumen fluid+ NP treatment+ feed composite) samples were collected from the glass bottles at the beginning and at the end of an experiment for bacterial counts, and volatile fatty acids (VFAs) analysis. RESULTS: Compared to the control treatment the H2S and GHGs concentration reduction after 72 h of the tested nZnO levels varied between 4.89 to 53.65%. Additionally, 0.47 to 22.21% microbial population reduction was observed from the applied nZnO treatments. Application of nZnO at a rate of 1000 µg g- 1 have exhibited the highest amount of concentration reductions for all three gases and microbial population. CONCLUSION: Results suggest that both 500 and 1000 µg g- 1 nZnO application levels have the potential to reduce GHG and H2S concentrations.