Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Data Brief ; 4: 315-21, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26217808

ABSTRACT

In the present data article we report the in vitro and in vivo biocompatibility of fabricated nerve conduits described in Das et al. [1]. Green synthesised gold nanoparticles (GNPs) were evaluated for their cytotoxicity in rat Schwann cells (SCTM41). We also describe herein the adhesion and proliferation of Schwann cells over the nanofibrous scaffolds. Methods describing surgical implantation of conduits in a rat sciatic nerve injury model, confirming its accurate implantation as well as the porosity and swelling tendency of the nerve conduits are illustrated in the various figures and graphs.

2.
Biomaterials ; 62: 66-75, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26026910

ABSTRACT

We report a novel silk-gold nanocomposite based nerve conduit successfully tested in a neurotmesis grade sciatic nerve injury model in rats over a period of eighteen months. The conduit was fabricated by adsorbing gold nanoparticles onto silk fibres and transforming them into a nanocomposite sheet by electrospinning which is finally given a tubular structure by rolling on a stainless steel mandrel of chosen diameter. The conduits were found to promote adhesion and proliferation of Schwann cells in vitro and did not elicit any toxic or immunogenic responses in vivo. We also report for the first time, the monitoring of muscular regeneration post nerve conduit implantation by recording motor unit potentials (MUPs) through needle electromyogram. Pre-seeding the conduits with Schwann cells enhanced myelination of the regenerated tissue. Histo-morphometric and electrophysiological studies proved that the nanocomposite based conduits pre-seeded with Schwann cells performed best in terms of structural and functional regeneration of severed sciatic nerves. The near normal values of nerve conduction velocity (50 m/sec), compound muscle action potential (29.7 mV) and motor unit potential (133 µV) exhibited by the animals implanted with Schwann cell loaded nerve conduits in the present study are superior to those observed in previous reports with synthetic materials as well as collagen based nerve conduits. Animals in this group were also able to perform complex locomotory activities like stretching and jumping with excellent sciatic function index (SFI) and led a normal life.


Subject(s)
Guided Tissue Regeneration/instrumentation , Nanocomposites/chemistry , Nerve Regeneration/physiology , Peripheral Nerve Injuries/physiopathology , Peripheral Nerve Injuries/therapy , Silk/chemistry , Equipment Design , Equipment Failure Analysis , Gold/chemistry , Materials Testing , Metal Nanoparticles/chemistry , Metal Nanoparticles/ultrastructure , Nanocomposites/ultrastructure , Neural Conduction/physiology , Peripheral Nerve Injuries/diagnosis , Recovery of Function/physiology , Schwann Cells/physiology , Schwann Cells/transplantation , Silk/ultrastructure , Tissue Scaffolds , Treatment Outcome
SELECTION OF CITATIONS
SEARCH DETAIL
...