ABSTRACT
In the era of miniaturization of electronic equipment and the need to connect sensors with textile materials, including clothing, the processing of signals received from the implemented sensors becomes an important issue. Information obtained by measuring the electrical properties of the sensors must be sent, processed, and visualized. For this purpose, the authors of this article have developed a prototype of a data collector obtained from textronic sensors created on composite textile substrates. The device operates in a system consisting of an electronic module based on the nRF52 platform, which supports wireless communication with sensors using Bluetooth technology and transmits the obtained data to a database hosted on the Microsoft Azure platform. A mobile application based on React Native technology was created to control the data stream. The application enables automatic connection to the selected collector, data download and their presentation in the form of selected charts. Initial verification tests of the system showed the correctness and reliability of its operation, and the presented graphs created from the obtained data indicate the usefulness of the device in applications where measurements and recording of impedance, resistance, and temperature are necessary. The presented prototype of a data collector can be used for resistance, impedance, and temperature measurements in the case of textronic structures but also in other wearable electronic systems.
ABSTRACT
Although the primary biological function of retina photoreceptors is to absorb light and provide visual information, exposure to intense light could increase the risk of phototoxic reactions mediated by rhodopsin photobleaching products (RPBP) that might accumulate in photoreceptor outer segments (POS). Here we investigated whether quercetin can modify the phototoxic potential of RPBP under in vitro photic stress conditions. ARPE-19 cells or quercetin enriched cultures pre-loaded with rhodopsin-rich POS isolated from bovine retinas were irradiated with green light to photobleach rhodopsin, and subsequently with blue light. Survival of cells was determined by MTT assay and propidium iodide staining. Changes in mitochondrial membrane potential (MMP) were assessed by JC-1 staining. Protein hydroperoxides, formed by photosensitized oxidation, mediated by RPBP, were analyzed in cells and in a model system with bovine serum albumin (BSA), using the coumarin boronic acid fluorogenic probe. The effect of photic stress on specific phagocytosis of RPE cells was determined by flow cytometry. Photoreactivity of POS with and without quercetin was analyzed by EPR oximetry and EPR spin trapping. Cytotoxicity measurements and MMP analyses confirmed that supplementation with quercetin protected ARPE-19 cells against photic stress mediated by rhodopsin-rich POS. Quercetin significantly reduced the inhibitory effect of RPBP-mediated stress on POS phagocytosis and the RPBP ability to photooxidize cellular proteins or BSA. The data support the hypothesis that quercetin may efficiently diminish the phototoxic action of retinoids, necessary for restoring the phagocytic function of ARPE-19 cells.
