ABSTRACT
Cystic echinococcosis (CE) is a significant health problem in both human and veterinary medicine. It is caused by the tapeworm Echinococcus granulosus (E. granulosus). The objective of this study was to investigate molecular diversity of E. granulosus from the paraffin-embedded human (FFPE) tissue samples using sequencing of mitochondrial genes. Thirty-five FFPE tissue samples were collected from different regions of Guilan province, north of Iran. Demographic data were recorded using a questionnaire. Five sections (1 mm) of the tissue were prepared and deparaffined using xylene and ethanol methods. Molecular analysis was performed using the Nad1 and Cox1 genes using PCR and DNA sequencing. Totally, 25 cases (71.43%) were women and 10 cases (28.57%) were men. The most affected age group was 21-30 yr old. The most of cysts were isolated from the liver (n = 19; 54.29%) and others in the lung (n = 16; 45.71%). The Cox1 and Nad1 genes were successfully amplified in 16 (45.71%) and 12 (34.28%) DNA samples from FFPE tissue. Sequencing analysis revealed that all samples were E. granulosus sensu stricto complex (G1 and G3). In this study, E. granulosus sensu stricto complex G1 and G3 were identified in human hydatid cysts and showed the presence of sheep/dog cycle in human infection. This finding confirmed and completed previous studies on the geospatial distribution of E. granulosus sensu stricto complex G1 and G3 in the southern and coastal areas of the Caspian Sea region.
ABSTRACT
PURPOSE: Neospora caninum is an obligate intracellular apicomplexan protozoan that causes neurological disorders in dogs. This systematic review and meta-analysis targeted at evaluating the worldwide seroprevalence rate of N. caninum in dogs. METHODS: Five electronic databases, including PubMed, Scopus, Web of Science, Science Direct, and Google Scholar, until December 2018 were thoroughly searched for relevant English language articles. Papers were selected based on inclusion criteria. Data were analyzed in StatsDirect statistical software (version 2.7.9). RESULTS: The searching process yielded a total number of 156 papers. The total prevalence (95% confidence intervals) of N. caninum infection in dog population worldwide was measured as 17.14% in data analyses (15.25-19.10). In addition, a significant association was also detected between neosporosis infection in dogs and continent, country, year, WHO regions, sample size and diagnostic method (P < 0.001). CONCLUSION: Dogs are a matter of concern to public health officials as a source of N. caninum infection. Therefore, it is of paramount importance to provide comprehensive data and systematically analyze the prevalence of N. caninum infection in dogs. Accordingly, it is recommended that researchers carry out further studies to prevent and control neosporosis among dog population worldwide.
