ABSTRACT
The aim of this project is to analyze how two families (one is living with a senior with physical disabilities and the other is living with seniors) feel about using the human-type communication robot "Palro" and what they demand for the improvement through their 3 weeks usage. All of them liked Palro and its programs, but needed some new programs. They pointed out that Palro sometimes had problems in the facial or voice recognition systems. Palro is useful in the area of self-care and social isolation.
Subject(s)
Activities of Daily Living , Caregivers , Communication Aids for Disabled , Mobility Limitation , Robotics/instrumentation , Self Care/instrumentation , Social Isolation , Aged , Female , Humans , Internet , Patient Satisfaction , SoftwareABSTRACT
Next-generation sequencing experiments have shown that microRNAs (miRNAs) are expressed in many different isoforms (isomiRs), whose biological relevance is often unclear. We found that mature miR-21, the most widely researched miRNA because of its importance in human disease, is produced in two prevalent isomiR forms that differ by 1 nt at their 3' end, and moreover that the 3' end of miR-21 is posttranscriptionally adenylated by the noncanonical poly(A) polymerase PAPD5. PAPD5 knockdown caused an increase in the miR-21 expression level, suggesting that PAPD5-mediated adenylation of miR-21 leads to its degradation. Exoribonuclease knockdown experiments followed by small-RNA sequencing suggested that PARN degrades miR-21 in the 3'-to-5' direction. In accordance with this model, microarray expression profiling demonstrated that PAPD5 knockdown results in a down-regulation of miR-21 target mRNAs. We found that disruption of the miR-21 adenylation and degradation pathway is a general feature in tumors across a wide range of tissues, as evidenced by data from The Cancer Genome Atlas, as well as in the noncancerous proliferative disease psoriasis. We conclude that PAPD5 and PARN mediate degradation of oncogenic miRNA miR-21 through a tailing and trimming process, and that this pathway is disrupted in cancer and other proliferative diseases.
