Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Tohoku J Exp Med ; 196(3): 193-201, 2002 Mar.
Article in English | MEDLINE | ID: mdl-12002276

ABSTRACT

The retinoblastoma protein-interacting zinc finger gene (RIZ) is a zinc-finger type DNA binding protein and is postulated as a member of the nuclear protein-methyltransferase superfamily. RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the N-terminal PR (PRDI-BF1 and RIZ homologous)-domain, RIZ2 lacks it. RIZ1 is now considered as a tumor suppressor. We analyzed nucleotide alteration of RIZ gene in human leukemia. The results revealed a single nucleotide polymorphism (SNP), T1704 to A, near the conserved Rb-binding domain, leading to an amino acid change, Asp283 to Glu. Interestingly, 17 of 21 leukemia cell lines are homozygous for the T1704 allele whereas only 2 of 20 normal subjects are homozygous for the allele. In addition, one base pair deletion in the poly (A)9 tract in the coding region near the C-terminal zinc-fingers was identified, resulting in frameshift, in 1 out of 17 leukemia cell lines, but no mutation in samples from 15 patients with acute lymphoblastic leukemia (ALL) and 6 patients with adult T cell leukemia (ATL). In the PR or SH3 (src homology 3) domain of the RIZ gene, no mutation was found. These findings suggest that RIZ may be a possible target of structural alteration leading to leukemia.


Subject(s)
DNA-Binding Proteins , Leukemia/genetics , Nuclear Proteins/genetics , Retinoblastoma Protein/metabolism , Transcription Factors , Alleles , Histone-Lysine N-Methyltransferase , Humans , Leukemia/metabolism , Mutation , Nuclear Proteins/metabolism , Polymorphism, Genetic , Retinoblastoma Protein/genetics , Tumor Cells, Cultured , Zinc Fingers
SELECTION OF CITATIONS
SEARCH DETAIL
...