Liquid base cytology in evaluation of thyroid nodules.

BACKGROUND: Palpable thyroid nodules are present in 4-7% of general population and Fine Needle Aspiration (FNA) is now accepted by endocrinologists and thyroid surgeons as a safe, simple and cost effective procedure for evaluating a thyroid nodule. The obtained sample can be spread directly on slides, processed as cell block preparations or prepared as liquid base smears. Liquid base method has been recently accepted due to its shorter preparation time and better preservation of nuclear details. The aim of this study is to compare the diagnostic results of two commonly used methods: Liquid Base Preparation and Cell Block Preparation in evaluation of thyroid nodules. METHODS: The samples were taken from 100 patients with a solitary nodule or a prominent nodule on a multinodular goiter background (excluding hot nodules). The obtained samples were used to prepare conventional smears (CS), Cell Block Preparations (CBP) and Liquid Base Preparations (LBP). The slides were studied by two pathologists, considering the following parameters: Cellularity, Colloid, Lymphocytes/Plasma cells and Macrophages. RESULTS: 87% of cases revealed informative results in LBP method while in the same group of patients only 69% of samples were informative after processing by CBP method. Sensitivity and specificity of both methods compared with the conventional smears and with each other and it is concluded that LBP is a reliable method for evaluating of a thyroid nodule. Other studies also show the same results. CONCLUSION: The liquid base method should be trusted due to its easier procedure, cleaner slide background, its higher specificity and higher diagnostic yields. It can be used instead of CBP and in association with CS to increase the accuracy of evaluation of thyroid nodules.

Older adult psychiatric inpatients with non-cognitive disorders should be screened for vitamin B12 deficiency.

OBJECTIVE: Vitamin B12 (B12) deficiency is most prevalent among older adults. Practice guidelines recommend screening older adults with symptoms of cognitive disorder for B12 deficiency. However, guidelines for non-cognitive psychiatric disorders typically do not mention screening older adults for B12 deficiency. The purpose of this study was to determine whether routine screening of older adult psychiatric inpatients for B12 deficiency, regardless of cognitive symptoms, is clinically justified. DESIGN: We conducted a retrospective chart-review study of consecutive inpatient admissions. SETTING: Older Adult Acute Psychiatric Inpatient Unit at the University of Maryland Medical Center from 10/2007-4/2010. PARTICIPANTS: Acute psychiatric inpatients aged ≥50 years who met inclusion criteria (N=374). MEASUREMENTS: Mean (SD) B12 levels and percentages of probable (<180pg/mL) and possible (180-350pg/mL) B12 deficiency as well as characteristics of patients with probable and possible B12 deficiency compared to patients with optimal B12 levels. RESULTS: Mean (SD) B12 levels and percentages of probable and possible B12 deficiency, respectively, for cognitive disorder patients [468 (284) pg/mL, 7.8 % (n=5) and 29.7% (n=19)] and for non-cognitive disorder patients [481(268) pg/mL, 4.8 %(n=15) and 33.2%( n=103)] were not significantly different (t=0.339, df=372, P=0.735; χ2=1.084, df=2, P=0.582, respectively). CONCLUSION: Considering the potential benefits and low costs of screening and treatment, we conclude that it is justified to routinely screen older adult psychiatric inpatients for B12 deficiency whether or not cognitive disorder symptoms are present.

Multimodal microglia imaging of fiber tracts in acute subcortical stroke.

OBJECTIVE: Case series with (11)C-PK11195 and positron emission tomography (PET) in stroke patients suggest that activated microglia may be detected in remote brain regions with fiber tract connections to the lesion site as an indicator of poststroke neuroinflammation. However, the specificity of these imaging findings remains to be demonstrated. METHODS: In a prospective controlled study, we measured microglia activity using (11)C-PK11195-PET along the pyramidal tract, as defined by diffusion tensor imaging, in 21 patients with first-time acute subcortical ischemia within 2 weeks of stroke. Uptake ratios (affected vs unaffected side) were determined for a set of standardized volumes of interest along the pyramidal tracts (PT). Uptake ratios from patients in whom the PT was affected were compared with those in whom the PT was not affected. Uptake ratios were related to motor deficit and lesion size according to correlation analyses. RESULTS: Increased uptake ratios were only found in patients in whom the PT was affected by stroke. In the affected hemisphere, uptake was increased at the level of pons, midbrain, and internal capsule, but not in the oval center. The extent of remote microglia activation was independent of infarct size or clinical measures of stroke severity. INTERPRETATION: A specific activation of microglia was only found in patients in whom the PT was affected by the stroke and only caudal (anterograde) to the lesion; no activation was found in the retrograde direction or in those patients in whom the PT was not affected. These findings were independent of infarct size and may represent changes secondary to early Wallerian degeneration.

Maternal beliefs and attitudes concerning diarrhoeal disease.

PIP: This letter report on an extension of a study conducted in Simla hills India which seeks to identify maternal beliefs and attitudes concerning diarrheal disease. The respondents are predominantly rural or periurban, illiterate slum mothers who were interviewed by a doctor according to a pretested, multiterm questionnaire. Preliminary observations, based on 600 cases, show that about 15% of the resondents do not understand the nature of diarrhea, although most such mothers admit to seeking hospital advice regarding a child's bowel ailment. Almost 1/2 blame diarrhea and the associated pain and irritability on teething as well as heat, cold, rains, worms, and faulty feeding of the mother if the infant is breastfed. About 10% of the mothers try witchcraft before seeking medical advice. Milk, routine feeds, and fluids are believed to aggravate diarrhea by certain numbers of the mothers. However, many believe that highly diluted milk is useful in addition to gruel or dal (a lentil). Almost 1/2 restrict the food or fluid intake of children with diarrhea, and some stop breastfeeding. Some 30% gave children home remedies. About 25% are aware of oral rehydration therapy, but only 1/2 are convinced of the value and few know how to prepare the solution properly. More studies in various segments of developing country populations on how to counter ignorance of ORT need to be done. Also there is a need for a result-oriented health education strategy flexible enough to adapt to different situations. A multi-media approach is the best way of informing, changing behavior, and motivating people, but such programs are felt to be too expensive for developing countries. Alternative approaches need to be evaluated.^ieng