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1.
J Thromb Haemost ; 14(12): 2496-2508, 2016 12.
Article in English | MEDLINE | ID: mdl-27706906

ABSTRACT

Essentials Stimulating endogenous fibrinolysis could be a novel antithrombotic strategy. The effect of valproic acid on endothelial tissue plasminogen activator in mice was investigated. Valproic acid increased tissue plasminogen activator expression in vascular endothelium. Valproic acid reduced fibrin deposition and thrombus formation after vascular injury. SUMMARY: Background The endogenous fibrinolytic system has rarely been considered as a target to prevent thrombotic disease. Tissue-type plasminogen activator (t-PA) production is potently increased by histone deacetylase (HDAC) inhibitors in endothelial cells in vitro, but whether this translates into increased vascular t-PA production and an enhanced fibrinolytic capacity in vivo is unknown. Objectives To determine whether the HDAC inhibitor valproic acid (VPA) stimulates production of t-PA in the vasculature of mice, and whether VPA pretreatment affects fibrin deposition and clot formation after mechanical vessel injury. Methods Mice were injected with VPA twice daily for up to 5 days. t-PA mRNA, and antigen expression in the mouse aorta and the circulating levels of t-PA were determined. Fibrin and thrombus dynamics after mechanical vessel injury were monitored with intravital confocal microscopy. Potential effects of VPA on platelets and coagulation were investigated. Results and Conclusions We found that VPA treatment increased vascular t-PA production in vivo and, importantly, that VPA administration was associated with reduced fibrin accumulation and smaller thrombi in response to vascular injury, but still was not associated with an increased risk of bleeding. Furthermore, we observed that higher concentrations of VPA were required to stimulate t-PA production in the brain than in the vasculature. Thus, this study shows that VPA can be dosed to selectively manipulate the fibrinolytic system in the vascular compartment and reduce thrombus formation in vivo.


Subject(s)
Endothelium, Vascular/metabolism , Thrombosis/drug therapy , Tissue Plasminogen Activator/metabolism , Valproic Acid/pharmacology , Animals , Aorta/metabolism , Blood Coagulation , Blood Platelets/metabolism , Enzyme Inhibitors/pharmacology , Fibrinolysis , Hemorrhage , Hippocampus/metabolism , Immunohistochemistry , Male , Mice , Mice, Inbred C57BL , Microscopy, Confocal , Platelet Function Tests , RNA, Messenger/metabolism
2.
Behav Brain Res ; 286: 33-8, 2015 Jun 01.
Article in English | MEDLINE | ID: mdl-25721743

ABSTRACT

Traumatic brain injury (TBI) represents a significant global health burden and causes long-lasting neuromotor deficits, particularly in individuals who sustain severe TBI. A better understanding of gait impairment after experimental TBI will provide valuable information for the recovery and rehabilitation of TBI survivors. Here we utilised the DigiGait system to perform kinematic gait analysis in mice subjected to brain injury induced by the controlled cortical impact (CCI) TBI model. Naïve mice, non-craniotomised and craniotomised mice were included as controls. The temporal and spatial profile of gait was mapped from 3h to 1-week post-TBI. Remarkably, there was a noticeable alteration in some aspects of gait in craniotomised sham mice from their pre-surgery baseline at various time-points over the testing period. This was not observed in naïve mice or non-craniotomised sham controls over the same time period. This finding indicates that the craniotomy procedure alone effects gait. When craniotomised mice were subjected to TBI, additional deleterious effects on gait function were observed, including forelimb stance and swing duration as well as left hindlimb swing and stride duration and frequency. Hence, mice subjected to CCI-induced TBI develop clear alterations in gait but part of this is attributable to the effect of craniotomy alone. This study also highlights the need to include both non-craniotomised and craniotomised sham mice as controls when undertaking the CCI-induced model of TBI, particularly when early time points are being evaluated.


Subject(s)
Brain Injuries/physiopathology , Gait , Movement Disorders/diagnosis , Movement Disorders/physiopathology , Animals , Biomechanical Phenomena , Brain Injuries/complications , Craniotomy , Disease Models, Animal , Forelimb/physiopathology , Gait/physiology , Hindlimb/physiopathology , Male , Mice, Inbred C57BL , Movement Disorders/etiology
3.
Cell Death Dis ; 5: e1410, 2014 Sep 11.
Article in English | MEDLINE | ID: mdl-25210793

ABSTRACT

Platelet activation is a frontline response to injury, not only essential for clot formation but also important for tissue repair. Indeed, the reparative influence of platelets has long been exploited therapeutically where application of platelet concentrates expedites wound recovery. Despite this, the mechanisms of platelet-triggered cytoprotection are poorly understood. Here, we show that activated platelets accumulate in the brain to exceptionally high levels following injury and release factors that potently protect neurons from apoptosis. Kinomic microarray and subsequent kinase inhibitor studies showed that platelet-based neuroprotection relies upon paracrine activation of the epidermal growth factor receptor (EGFR) and downstream DNA-dependent protein kinase (DNA-PK). This same anti-apoptotic cascade stimulated by activated platelets also provided chemo-resistance to several cancer cell types. Surprisingly, deep proteomic profiling of the platelet releasate failed to identify any known EGFR ligand, indicating that activated platelets release an atypical activator of the EGFR. This study is the first to formally associate platelet activation to EGFR/DNA-PK--an endogenous cytoprotective cascade.


Subject(s)
Apoptosis , Blood Platelets/enzymology , Brain Injuries/enzymology , DNA-Activated Protein Kinase/metabolism , ErbB Receptors/metabolism , Neurons/cytology , Adolescent , Adult , Aged , Animals , Blood Platelets/metabolism , Brain/cytology , Brain/enzymology , Brain Injuries/genetics , Brain Injuries/physiopathology , Cell Line, Tumor , Cells, Cultured , DNA-Activated Protein Kinase/genetics , ErbB Receptors/genetics , Female , Humans , Male , Mice, Inbred C57BL , Middle Aged , Platelet Activation , Young Adult
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