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1.
Biosci Biotechnol Biochem ; 78(11): 1954-62, 2014.
Article in English | MEDLINE | ID: mdl-25351334

ABSTRACT

Low protein content and sufficient grain rigidity are desired properties for the rice used in high-quality sake brewing such as Daiginjo-shu (polishing ratio of the rice, less than 50%). Two kinds of rice, sake rice (SR) and cooking rice (CR), have been used for sake brewing. Compared with those of SR, analyses of CR for high-quality sake brewing using highly polished rice have been limited. Here we described the original screening of late-maturing CR Sensyuraku (SEN) as rice with low protein content and characterization of its properties for high-quality sake brewing. The protein content of SEN was lower than those of SR Gohyakumangoku (GOM) and CR Yukinosei (YUK), and its grain rigidity was higher than that of GOM. The excellent properties of SEN with respect to both water-adsorption and enzyme digestibility were confirmed using a Rapid Visco Analyzer (RVA). Further, we confirmed a clear taste of sake produced from SEN by sensory evaluation. Thus, SEN has excellent properties, equivalent to those of SR, for high-quality sake brewing.


Subject(s)
Alcoholic Beverages , Fermentation , Oryza/chemistry , Oryza/metabolism , Cooking
2.
Biosci Biotechnol Biochem ; 77(10): 2160-5, 2013.
Article in English | MEDLINE | ID: mdl-24096657

ABSTRACT

The Japanese high-quality sake Daiginjo-shu is made from highly polished rice (polishing ratio, less than 50%). Here we showed that the sake rice Koshitanrei (KOS) has an excellent polishing property. Rice grains of KOS had the same lined white-core region as the sake rice Yamadanishiki (YAM). The grain rigidity/hardness of KOS was higher than that of the sake rice Gohyakumangoku (GOM). The loss ratio of KOS after high polishing by an industrial polishing machine was lower than that of GOM. Further, a clear taste of sake produced from KOS was confirmed by sensory evaluation.


Subject(s)
Alcoholic Beverages/standards , Food Handling/methods , Oryza , Mechanical Phenomena , Oryza/classification , Time Factors
3.
J Virol ; 86(9): 5264-77, 2012 May.
Article in English | MEDLINE | ID: mdl-22357273

ABSTRACT

Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, is a major virion tegument protein. In the present study, we showed that VP22 was required for efficient redistribution of viral proteins VP16, VP26, ICP0, ICP4, and ICP27 and of cellular protein Hsc-70 to the cytoplasm of infected cells. We found that two dileucine motifs in VP22, at amino acids 235 and 236 and amino acids 251 and 252, were necessary for VP22 regulation of the proper cytoplasmic localization of these viral and cellular proteins. The dileucine motifs were also required for proper cytoplasmic localization of VP22 itself and for optimal expression of viral proteins VP16, VP22, ICP0, UL41, and glycoprotein B. Interestingly, a recombinant mutant virus with alanines substituted for the dileucines at amino acids 251 and 252 had a 50% lethal dose (LD(50)) for neurovirulence in mice following intracerebral inoculation about 10(3)-fold lower than the LD(50) of the repaired virus. Furthermore, the replication and spread of this mutant virus in the brains of mice following intracerebral inoculation were significantly impaired relative to those of the repaired virus. The ability of VP22 to regulate the localization and expression of various viral and cellular proteins, as shown in this study, was correlated with an increase in viral replication and neurovirulence in the experimental murine model. Thus, HSV-1 VP22 is a significant neurovirulence factor in vivo.


Subject(s)
Herpesvirus 1, Human/metabolism , Herpesvirus 1, Human/pathogenicity , Proteins/metabolism , Viral Structural Proteins/metabolism , Amino Acid Motifs , Amino Acid Substitution , Animals , Brain/pathology , Brain/virology , Cell Line , Chlorocebus aethiops , Female , Gene Order , Genome, Viral , Herpes Simplex Virus Protein Vmw65/genetics , Herpes Simplex Virus Protein Vmw65/metabolism , Herpesvirus 1, Human/genetics , Leucine/chemistry , Mice , Mice, Inbred ICR , Mutation , Protein Transport , Proteins/genetics , Rabbits , Viral Proteins/genetics , Viral Proteins/metabolism , Viral Structural Proteins/genetics , Virulence , Virus Replication/genetics
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