ABSTRACT
A 20-year-old woman with ulcerative colitis flare was admitted to Fukui Red Cross Hospital. During treatment with granulocyte and monocyte apheresis (GMA), the patient complained of chest discomfort and was diagnosed with pulmonary thromboembolism with central intravenous catheter thrombosis. Apixaban, a direct oral anticoagulant (DOAC), was used as monotherapy for anticoagulation, and thromboembolism was resolved without complication. Among extraintestinal complications in patients with ulcerative colitis, pulmonary thromboembolism can be a life-threatening condition and requires prompt anticoagulant therapy. Although the effectiveness of conventional pharmacological anticoagulation using warfarin and heparin has been reported, the efficacy of DOAC monotherapy remains controversial. DOAC monotherapy may be considered and innovative therapeutic strategy for a thromboembolic condition in patients with ulcerative colitis.
Subject(s)
Colitis, Ulcerative , Pulmonary Embolism , Thrombosis , Anticoagulants/therapeutic use , Colitis, Ulcerative/complications , Colitis, Ulcerative/drug therapy , Female , Humans , Pulmonary Embolism/drug therapy , Pulmonary Embolism/etiology , Pyrazoles , Pyridones , Young AdultABSTRACT
BACKGROUND: Helicobacter pylori CagA protein is considered to be one of the virulence factors associated with gastric cancer. CagA is injected into gastric epithelial cells, undergoes tyrosine phosphorylation, and binds to Src homology 2 domain-containing protein-tyrosine phosphatase (SHP-2). Two major subtypes of CagA have been observed in the SHP-2-binding site, the Western and East Asian types. The East Asian-type CagA binds to SHP-2 more strongly than the Western-type CagA. The diversity of CagA, which collectively determines the binding affinity of CagA to SHP-2, may be an important variable in determining the clinical outcome of infection by different H. pylori strains. METHODS: We investigated the relationship between the diversity of CagA and clinical outcome in Okinawa, Japan. A total 24 strains, 13 gastric cancer strains and 11 duodenal ulcer strains, were studied. We sequenced full-length cagA genes and analyzed the phylogenetic relationships between Okinawa isolates and previously characterized Western H. pylori strains. RESULTS: All isolates examined were cagA positive. The prevalence of East Asian CagA-positive strains was significantly higher in patients with gastric cancer (84.6%) than in patients with duodenal ulcer (27.3%) (chi-squared = 8.06, P = 0.011). The phylogenetic analysis showed that all gastric cancer strains with East Asian-type CagA were in the East Asian cluster, and that most duodenal ulcer strains were in the Western cluster. CONCLUSIONS: The origins of H. pylori isolates are different between gastric cancer strains and duodenal ulcer strains, and East Asian CagA-positive H. pylori infection is associated with gastric cancer. The strain diversity observed in Okinawa may affect the difference in the prevalence of disease associated with H. pylori infection in Japan.
Subject(s)
Antigens, Bacterial/genetics , Bacterial Proteins/genetics , Duodenal Ulcer/microbiology , Genetic Variation , Helicobacter Infections/microbiology , Helicobacter pylori/genetics , Stomach Neoplasms/microbiology , Adult , Aged , Aged, 80 and over , Antigens, Bacterial/classification , Bacterial Proteins/classification , Duodenal Ulcer/epidemiology , Female , Helicobacter Infections/epidemiology , Helicobacter pylori/classification , Helicobacter pylori/isolation & purification , Helicobacter pylori/pathogenicity , Humans , Japan/epidemiology , Male , Middle Aged , Phylogeny , Prevalence , Sequence Analysis, DNA , Stomach Neoplasms/epidemiologyABSTRACT
BACKGROUND: Interleukin-1beta (IL-1beta) gene polymorphisms are related to hypochlorhydria and increase the risk of gastric cancer in the presence of Helicobacter pylori infection. However, little information is available about the genetic risk factors of reflux esophagitis. In this study we investigated its association with the IL-1beta polymorphisms. METHODS: We examined 48 patients with reflux esophagitis and 96 control subjects, 89 with gastric cancer. IL-1beta-511C/T genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: The frequency of IL-1beta-511T alleles was significantly higher in reflux esophagitis patients (57.3%) than in controls (41.1%) (P = 0.0215, chi(2) = 5.289). The frequency of IL-1beta-511T/T genotypes was also significantly higher in reflux esophagitis patients (31.3%) than in controls (15.6%). The odds ratio and the 95% confidence interval were 4.000 and 1.393-11.486, respectively. The frequency of IL-1beta-511T/T genotypes was significantly higher in reflux esophagitis patients (31.3%) than in gastric cancer patients (21.4%). The odds ratio and the 95% confidence interval were 2.961 and 1.054-8.316, respectively. CONCLUSIONS: IL-1beta-511T was associated with reflux esophagitis having hyperacidity. Differences of genetic background regarding gastric acid secretion may exist between Japanese and Caucasians.
