Surface Activated Pyrolytic Carbon Black: A Dual Functional Sustainable Filler for Natural Rubber Composites.

The significant rise in end-of-life tires (ELTs) globally poses immediate environmental and human health risks. Therefore, to promote ELTs recycling and to reduce tire industry carbon emissions, herein we present a facile approach for fine-tuning the interfacial interactions between pyrolytic carbon black (P-CB) obtained from ELTs and natural rubber (NR) matrix using phosphonium-based ionic liquid (PIL). The reinforcing effect of PIL-activated P-CB was studied by replacing the furnace-grade carbon black (N330-CB) with varying PIL and P-CB loadings. Adding PIL improved the filler dispersion and the cross-linking kinetics with a substantially reduced zinc oxide (ZnO) loading. Considering the cross-linking and viscoelastic properties, it was concluded that the composite, P-CB/N330-CB-PIL (1.5)+ZnO (1) with half substitution of N330-CB with P-CB synergistically works with 1.5 phr PIL and 1 phr of ZnO resulting in improved dynamic-mechanical properties with a minimal loss tangent value at 60 °C (tanδ=0.0689) and improved glass transition temperature (Tg =-38 °C) compared to control composite. The significant drop (~29 % lower) in tanδ could reduce fuel consumption and related CO2 emissions. We envisage that this strategy opens an essential avenue for "Green Tire Technology" towards the substantial pollution abatement from ELTs and reduces the toxic ZnO.

Molecular Insights into Antioxidant Efficiency of Melanin: A Sustainable Antioxidant for Natural Rubber Formulations.

N-(1,3-Dimethyl butyl)-N'-phenyl-p-phenylenediamine (6-PPD) is a worldwide antioxidant commonly added to delay the thermo-oxidative degradation of tire rubbers. Unfortunately, 6PPD and its transformation product 6PPD-quinone are toxic to aquatic organisms (e.g., coho salmon). Herein, we explore the free radical scavenging activity and protective mechanism of melanin (MLN) on natural rubber's (NR's) oxidative resistance using molecular dynamics (MD) and quantum mechanical (QM) calculations. The relationship between the molecular structure and the chemical nature of the antioxidant molecules via transition state calculations is explored to unravel the reaction mechanisms of antioxidants interacting with peroxy radicals (ROO·) of NR with the estimation of reaction barriers. Following this, the radical scavenging activity of antioxidants was quantified via a hydrogen atom transfer mechanism and bond dissociation energy calculations. Parallel MD simulations were considered to study the interfacial interactions of antioxidant molecules with polymer chains and fillers with a quantifiable structure-property correlation. Given these results, the nanocomposite (NR-MLN-SiO2) with natural antioxidant melanin manifested outstanding antioxidant properties by preferentially bagging the ROO· radicals, thus improving NR's thermal-oxidative aging relative to 6-PPD. The MD results revealed that the intermolecular interactions at the NR/antioxidant interface benefited the antioxidant MLN to bind tightly to the NR in NR-MLN-SiO2 composite, thus exhibiting improved dispersion, O2 barrier properties, and thermo-oxidative stability, which could extend the service life of NR products (e.g., tires). In addition, as a sustainable antioxidant, MLN could replace toxic antioxidants like 6-PPD. More importantly, the QM/MD simulations provided a fundamental understanding of the mechanistic pathways of antioxidant molecules in NR composites, which are conducive to designing high-performance and sustainable green elastomers.

Phosphonium Ionic Liquid-Activated Sulfur Vulcanization: A Way Forward to Reduce Zinc Oxide Levels in Industrial Rubber Formulations.

Extensive use of zinc oxide and accelerators such as diphenyl guanidine (DPG) in the vulcanization of rubber composites entail potential environmental risks. These are pervasive contaminants of roadway runoff originating from tire wear particles (TWPs). Herein, the effect of phosphonium ionic liquids (PILs) in styrene-butadiene rubber compounds was demonstrated with reduced ZnO loading and no DPG to minimize the environmental footprint of the vulcanization process. The structure and chemistry of PILs were found to be the influencing parameters impelling the cross-linking kinetics, enabling shorter induction times. The generation of active Zn2+ sites by PILs was examined through FTIR spectroscopy, calorimetry, and molecular dynamics simulations. From a tire application perspective, the PILs not only enhanced the cure kinetics but also improved the dynamic-mechanical behavior of the rubber composites. Consequently, the harm caused by TWPs to the atmosphere, fuel intake, and CO2 emissions was minimal, thereby confirming the potential use of PILs in the tire industry.

Association of Prior Anterior Cruciate Ligament Tear With Decreased Career Longevity in Women's National Basketball Association.

BACKGROUND: The incidence of anterior cruciate ligament (ACL) injuries in women's basketball exceeds that of men. There is a paucity of data regarding career performance in Women's National Basketball Association (WNBA) athletes with a history of ACL reconstruction. PURPOSE: To determine whether WNBA athletes with a history of ACL injury prior to professional play have reduced career game utilization, defined as games played and started and minutes per game (MPG), as well as statistical performance, defined by player efficiency rating (PER). STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: Included were 42 WNBA players from 1997 to 2018 who had a history of ACL reconstruction before entering professional leagues. Body mass index (BMI), age, and position were collected for each player. Career data and performance statistics were likewise collected for each player's entire WNBA career. A control group of WNBA players with no history of ACL injury were matched by position, BMI, and age at the time of WNBA debut. Statistics compared game utilization and performance to assess the impact of ACL reconstruction. RESULTS: Athletes who sustained an ACL tear before entering the league played in fewer games per season in their first 3 professional seasons compared with healthy controls (24.2 ± 8.4 vs 28.2 ± 6.1; P = .02). Among athletes with a history of ACL reconstruction, 11 (26.2%) played only a single WNBA season, while no control athletes played in just 1 season. Additionally, athletes who had a previous ACL tear started significantly fewer games per season (9.0 ± 9.4 vs 14.0 ± 9.0; P < .01) and played fewer MPG (15.5 ± 7.2 vs 20.7 ± 5.5; P < .01) during their WNBA career. Athletes with a history of ACL tear had significantly shorter WNBA careers (4.8 ± 4.1 vs 8.1 ± 3.3 seasons; P < .001). Total professional play duration (WNBA + overseas) was significantly reduced in players with an ACL tear compared with controls (P < .05). PER was not significantly different between cohorts at any time point. CONCLUSION: WNBA athletes with a history of an ACL tear before professional play had decreased career game utilization and workload throughout their career despite having similar PER compared with healthy controls.

Design Principles of Interfacial Dynamic Bonds in Self-Healing Materials: What are the Parameters?

Polymers and polymer nanocomposites (PNCs) are extensively used in daily life. However, the growing requirement of advanced PNCs laid persistent environmental issues due to deformation-induced damage that once formed, does not vanish at future stages. Therefore, self-healing materials with significantly enhanced long life and safety have been designed to epitomize the forefront of recent advances in materials chemistry and engineering. Self-healing PNC (SH-PNCs) materials are a class of smart composites in which nanoparticles induce interfacial reconstruction via multiple covalent and non-covalent interactions culminating in improved mechanical strength and self-healing capability. However, since the filler nanoparticles are independent of the reversible supramolecular network, the filler incorporation destroys the self-healing ability but could enhance the mechanical strength. Hence, the molecular parameters controlling the alliance of robust mechanical strength with virtuous self-healing ability is a crucial challenge. Herein, we review the latest developments that have been made in self-healing materials and puts advancing insights into the fabrication of SH-PNCs in which the combination of covalent bonds and non-covalent interactions provides an optimal balance between their mechanical performance and self-healing capability. We highlight the importance of specific entropic, enthalpic changes, polymer chain conformations and flexibility that enable the reconstruction of damaged surface and physical reshuffling of dynamic bonds at the interface of cut surfaces.

Role of Interface Structure and Chain Dynamics on the Diverging Glass Transition Behavior of SSBR-SiO2-PIL Elastomers.

Intermolecular interactions between the constituents of a polymer nanocomposite at the polymer-particle interface strongly affect the segmental mobility of polymer chains, correlated with their glass transition behavior, and are responsible for the improved dynamical viscoelastic properties. In this work, we emphasized on the evolution of characteristic interfaces and their dynamics in silica (SiO2 NP)-reinforced, solution-polymerized, styrene butadiene rubber (SSBR) composites, whose relative prevalence varied with the phosphonium ionic liquid (PIL) volume fraction, used as an interfacial modifier. The molecular origins of such interfaces were examined through systematic dielectric spectroscopy, molecular dynamics (MD) simulations, and dynamic-mechanical analyses. The PIL facilitated H-bonding, cation-π, surface-phenyl, and van der Waals interfacial interactions between SSBR and SiO2 NP, thereby regulating the polymer chain dynamics, orientation, and mean-square displacement. Specifically, the mass density profiles from MD simulations revealed the dynamic gradient of polymer chains in the interfacial region as a function of radial distance from the center of mass of the SiO2 NP surface. The results showed a structuring effect to result in well-resolved density peaks at specific radial distances with the tangential orientation of styrene monomers in the vicinity of the SiO2 NP surface. These domino effects highlighted strong interfacial interactions to have an indispensable effect on the viscoelastic performance and thermal motion of SSBR molecular chains, leading to a higher glass transition temperature (T g) by ∼15 K, validating the experimental data. More importantly, our results gave new insights into the fundamental understanding of the fact that the strength of intermolecular interactions induced by PIL at the polymer-particle interface is the key to control the α-relaxation dynamics and T g optimization, desired for specific applications.

Design of Dual Hybrid Network Natural Rubber-SiO2 Elastomers with Tailored Mechanical and Self-Healing Properties.

The preparation of natural rubber (NR)-silica (SiO2) elastomeric composites with excellent mechanical properties along with better self-healing ability remains a key challenge. Inspired by the energy dissipation and repairability of sacrificial bonds in biomaterials, a strategy for combining covalent and noncovalent sacrificial networks is engineered to construct a dual hybrid network. Here, the approach used to fabricate the composites was self-assembly of NR, bearing proteins and phospholipids on its outer bioshell, with SiO2 via metal-ion-mediated heteroaggregation effected by reversible electrostatic and H-bonds. Further, covalent cross-links were incorporated by a silane coupling agent, bis [3-(triethoxysilyl) propyl] tetrasulfide. The intrinsic self-healing ability of the composite at the molecular level was studied by broadband dielectric spectroscopy that unraveled the mechanism of the healing process. The synergistic effect between the molecular interdiffusion of the cross-linked NR chains and the electrostatic and H-bonding interactions imparted an exceptional self-healing characteristic to the liquid-liquid-mixing-prepared NR-SiO2 composites with improved mechanical performance. Specifically, the segmental relaxation dynamics of the healed composite was largely restricted due to increased number of ion-dipole interactions and S-S cross-links at the junction of the cut surface. We envisage that this extraordinary healing property, unreported yet, would be of great importance toward the design of novel NR-SiO2 elastomeric hybrids with superior mechanical properties.

Epoxyeicosatrienoic Acid Analog EET-A Blunts Development of Lupus Nephritis in Mice.

Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disorder that causes life threatening renal disease and current therapies are limited with serious side-effects. CYP epoxygenase metabolites of arachidonic acid epoxyeicosatrienoic acids (EETs) demonstrate strong anti-inflammatory and kidney protective actions. We investigated the ability of an orally active EET analog, EET-A to prevent kidney injury in a mouse SLE model. Twenty-weeks old female NZBWF1 (SLE) and age-matched NZW/LacJ (Non SLE) were treated with vehicle or EET-A (10 mg/kg/d, p.o.) for 14 weeks and urine and kidney tissues were collected at the end of the protocol. SLE mice demonstrated marked renal chemotaxis with 30-60% higher renal mRNA expression of CXC chemokine receptors (CXCR) and CXC chemokines (CXCL) compared to Non SLE mice. In SLE mice, the elevated chemotaxis is associated with 5-15-fold increase in cytokine mRNA expression and elevated inflammatory cell infiltration in the kidney. SLE mice also had elevated BUN, serum creatinine, proteinuria, and renal fibrosis. Interestingly, EET-A treatment markedly diminished renal CXCR and CXCL renal mRNA expression in SLE mice. EET-A treatment also reduced renal TNF-α, IL-6, IL-1ß, and IFN-γ mRNA expression by 70-80% in SLE mice. Along with reductions in renal chemokine and cytokine mRNA expression, EET-A reduced renal immune cell infiltration, BUN, serum creatinine, proteinuria and renal fibrosis in SLE mice. Overall, we demonstrate that an orally active EET analog, EET-A prevents renal injury in a mouse model of SLE by reducing inflammation.

Assessing the Perceptions and Practice of Self-Medication among Bangladeshi Undergraduate Pharmacy Students.

Objectives: To evaluate the perceptions and extent of practicing self-medication among undergraduate pharmacy students. Methods: This cross-sectional, questionnaire-based study was conducted over a six month period (January to June 2016) among undergraduate pharmacy students in five reputable public universities of Bangladesh. It involved face-to-face interviews regarding self-medication of 250 respondents selected by simple random sampling. Results: Self-medication was reported by 88.0% of students. Antipyretics (58.40%) were mostly preferred for the treatment of fever and headaches. The major cause for self-medication was minor illness (59.60%, p = 0.73) while previous prescriptions were the main source of knowledge as well as the major factor (52.80%, p = 0.94) dominating the self-medication practice. The results also demonstrated 88.80% of students had previous knowledge on self-medication and 83.60% of students always checked the information on the label; mainly the expiry date before use (85.60%). A significant (p < 0.05) portion of the students (51% male and 43% female) perceived it was an acceptable practice as they considered self-medication to be a segment of self-care. Furthermore, students demonstrated differences in their response level towards the adverse effect of drugs, the health hazard by a higher dose of drug, a physician's help in case of side effects, taking medicine without proper knowledge, and stopping selling medicine without prescription. Conclusions: Self-medication was commonly used among pharmacy students primarily for minor illnesses using over-the-counter medications. Although it is an inevitable practice for them it should be considered an important public health problem as this practice may increase the misuse or irrational use of medicines.

Magnetic Resonance Spectroscopy: Novel Non-invasive Technique for Diagnosing Brain Tumors.

OBJECTIVE: To determine the accuracy of MR Spectroscopy (MRS) in diagnosing brain tumors. STUDY DESIGN: Analytical study. PLACE AND DURATION OF STUDY: Neurosurgery Department, Jinnah Postgraduate Medical Centre, Karachi, from November 2010 to April 2011. METHODOLOGY: Fifty cases with brain tumors, who presented to Neurosurgery Department of Jinnah Postgraduate Medical Centre, Karachi, during the study period, were included in the study. All patients underwent MRS and later brain. Those with recurrent disease were excluded. Data was collected with the help of proforma. Data was analyzed using SPSS version 16. Comparison of MRS findings and biopsy diagnosis was done. Sensitivity, specificity, negative and positive predictive values (NPV and PPV) were determined keeping histopathology as the gold standard. RESULTS: Out of the 50 patients, there were 20 (40%) females and 30 (60%) males with mean age of 37 ± 13.24 years. The commonest presenting complaint was headache (76%) followed by weakness (62%) and seizures (30%). MRI had diagnosed 27 (51%) as neoplastic lesion. Spectroscopy reported 44 (88%) as neoplasms, while on histopathology, 42 (84%) were confirmed to have neoplasm. The accuracy of MRS was 94%, with 97.6% sensitivity, 71.42% specificity, 95.45% PPV and 83.3% NPV. CONCLUSION: Magnetic resonance spectroscopy can readily help in differentiating neoplasm from non-neoplastic brain tumors, thus an invasive brain biopsy procedure can be avoided.

Rapid Clinical Course of Multiple Metastatic Cerebral Angiosarcoma.

Central nervous system (CNS) angiosarcoma, both primary and secondary, is an exceptionally rare malignant tumor. The behaviour of angiosarcoma is one of rapid progression with early metastatic spread but as with all sarcomas, the primary site may not clinically evident by the time metastases develop. Identification of the primary site of origin is masked by the carpet-like growth pattern that may occur in the left atrium, the most frequently encountered primary site for angiosarcoma. This rare tumor presents many diagnostic challenges. We describe the clinical and neuropathologic features of two patients with angiosarcoma of the brain and review the previously published cases described to date.

Evaluation of antibacterial and anthelmintic activities with total phenolic contents of Piper betel leaves.

OBJECTIVE: The study was conducted to investigate the antibacterial and anthelmintic activities and to determine total phenolic contents of methanolic extract of Piper betel leaves. MATERIALS AND METHODS: The extract was subjected to assay for antibacterial activity using both gram positive and gram negative bacterial strains through disc diffusion method; anthelmintic activity with the determination of paralysis and death time using earthworm (Pheritima posthuma) at five different concentrations and the determination of total phenolic contents using the Folin-ciocalteau method. RESULTS: The extract showed significant (p<0.01) zone of inhibitions against gram positive Staphylococcus aureus [(6.77±0.25) mm] and Gram negative Escherichia coli [(8.53±0.25) mm], Salmonella typhi [(5.20±0.26) mm], Shigella dysenteriae [(11.20±0.26) mm] compared to positive control Azithromycin (ranging from 20.10±0.17 to 25.20±0.35 mm) while no zone inhibitory activity was found for both the extract and the standard drug against Gram positive Bacillus cereus. The extract also showed potent anthelmintic activity requiring less time for paralysis and death compared to the standard drug albendazole (10 mg/ml). At concentrations 10, 20, 40, 60 and 80 mg/ml, leaves extract showed paralysis at mean time of 9.83±0.60, 8.50±0.29, 6.60±0.17, 6.20±0.44 and 4.16±0.60; death at 11.33±0.88, 9.67±0.33, 7.83±0.17, 7.16±0.60 and 5.16±0.72 minutes, respectively. Whereas the standard drug showed paralysis and death at 19.33±0.71 and 51.00±0.23 minutes respectively. The extract confirmed the higher concentration of phenolic contents (124.42±0.14 mg of GAE /g of extract) when screened for total phenolic compounds. CONCLUSION: As results confirmed potential antibacterial and anthelmintic activities of Piper betel leaves extract, therefore it may be processed for further drug research.

Prevalence of iron-deficiency anaemia among university students in Noakhali region, Bangladesh.

Iron-deficiency anaemia (IDA) is a common health problem in rural women and young children of Bangladesh. The university students usually take food from residential halls, and the food value of their diets is not always balanced. This cross-sectional study was conducted to estimate the prevalence of iron-deficiency anaemia among the university students of Noakhali region, Bangladesh. Haemoglobin level of 300 randomly-selected students was measured calorimetrically, using Sahli's haemoglobinometer during October to December 2011. Statistical analysis was done by using SPSS software for Windows (version 16) (SPSS Inc., Chicago, IL, USA). In the study, 55.3% students were found anaemic, of whom 36.7% were male, and 63.3% were female. Students aged 20-22 years were more anaemic (43.4%) than other age-groups. Majority (51.3%) of male students showed their haemoglobin level in the range of 13-15 g/dL, followed by 26.0% and 21.3% with 10-12 g/dL and 16-18 g/dL respectively. Although 50.5% anaemic and 51.1% non-anaemic female students showed normal BMI--lower percentage than anaemic (60.7%) and non-anaemic (71.9%) male students, the underweight students were found more anaemic than the overweight and obese subjects. Regular breakfast-taking habit showed significant (p = 0.035, 95% CI 0.5-1.0) influence on IDA compared to non-regular breakfast takers. Consumption of meat, fish, poultry, eggs, or peanut butter regularly; junk food; multivitamins; and iron/iron-rich food showed insignificant (p = 0.097, 95% CI 0.5-1.1; p = 0.053, 95% CI 1.1-2.3; p = 0.148, 95% CI 0.6-1.2; and p = 0.487, 95% CI 0.7-1.4 respectively) role in provoking IDA. In the case of non-anaemic subjects, all of the above parameters were significant, except the junk food consumption (p = 0.342, 95% CI 0.5-1.2). Our study revealed that majority of university students, especially female, were anaemic that might be aggravated by food habit and lack of awareness. The results suggest that anaemia can be prevented by providing proper knowledge on the healthful diet, improved lifestyle, and harmful effect of anaemia to the students.

Attitudes and perceptions of medical students toward neurosurgery.

OBJECTIVE: To evaluate the attitudes and perceptions of medical student toward neurosurgery. METHODS: A cross-sectional survey comprising questions based on a Likert scale was used to analyze the attitudes and perceptions of third-year, fourth-year, and fifth-year medical students from the Royal College of Surgeons in Ireland. An anonymous questionnaire containing 15 items was administered and scored following a Likert ranking scale (1, disagree; 2, agree somewhat; 3, agree moderately; 4, agree strongly). RESULTS: This prospective study included 60 students 20-26 years old (mean age, 23 years old). Most of the students agreed that their neurosurgery teaching is inadequate, neurosurgical history is difficult to obtain, neurosurgical signs are difficult to elicit, the neurosurgery training period is long, neurosurgical illnesses have poor outcomes, and neurosurgery can impede family life (70%-100%). CONCLUSIONS: The findings identify some areas that may be targeted to stimulate and improve medical students' interest and passion toward the pursuit of neurosurgery as a specialty and ultimately to improve their learning experience.

Intramedullary dermoid cyst with relatively atypical symptoms: a case report and review of the literature.

BACKGROUND: Intraspinal dermoid cysts are rare and benign tumors that occur primarily due to the defective closure of the neural tube, an ectodermal derivative, during the process of development. They are slow-growing tumors manifesting in the second and third decades of life. CASE PRESENTATION: We present here a case of a 14-year-old Sindhi boy with a six-month history of paraparesis of the lower limbs and a progressive loss of power of grade 3/5, and hypoesthesia in the L4/L5 dermatomes of his right lower limb. A plain magnetic resonance imaging scan revealed a well-demarcated intraspinal intramedullary cyst containing an abscess at the level of T12 and L1 causing localized cord compression, which was producing the symptoms. Near total excision of the cyst was successfully performed and was sent for biopsy, which revealed keratinocytes and keratin flakes. With one month of follow-up, along with physiotherapeutic management, the patient gradually improved and was able to walk without support. CONCLUSIONS: Critical evaluation of every case with aggravating symptoms should be carried out, and neurological and radiological examinations should be conducted to ensure the well-being of patients.

Delayed diagnosis of post-traumatic C7 vertebra anterior subluxation with an unusual neurological pattern: a case report.

INTRODUCTION: Post-traumatic subluxations are potentially devastating injuries to the axial skeleton. Of utmost priority are an expedient and timely diagnosis and realignment because of its association with spinal cord and nerve root trauma, which lead to progressive deleterious neurological deficits. A good radiological study of the occipitocervical joint and first thoracic vertebra is key to a successful early diagnosis. However, cases might still fail to be diagnosed, leading to trouble. A case of post-traumatic subluxation at the C7 vertebral level with an unusual neurological pattern is presented here. CASE PRESENTATION: A 35-year-old farmer from the Sindh province of Pakistan presented to our neurology department after a fall 2 months earlier and complained of lower limb pain and difficulty in walking. He had numbness in both of his lower limbs up to his umbilical region, with sparing of bladder function along with intact strength in the upper extremities bilaterally. CONCLUSIONS: Our case highlights the unusual sparing of upper limbs and intact urinary continence with severe lower limb deficits in a 70% subluxation. Our case is unusual because highly detrimental effects such as quadriplegia are expected with such extreme subluxation, but our patient presented with only lower limb deficits. This case serves as a reminder to emergency medicine doctors, spine surgeons, and even radiologists (a) to evaluate spine injuries by using computed tomography in trauma patients to identify artifact around a suspected injury and (b) to be mindful of negative conventional radiographs.

Unilateral facial palsy in an infant: an unusual presentation of familial multiple cerebral cavernous malformation.

Cerebral cavernous malformation (CCM) in infants tends to have genetic predisposition. These cavernomas have a progressive course of events and associated neurological symptoms with increase in age. They most commonly present with seizure and syndrome of increased intracranial pressure comprising of headache, vomiting and focal neurological signs. We describe a case of a 7-month-old infant who presented with an acute onset of right facial paralysis with a background of familial CCM. The CT and MRI scan revealed fresh haemorrhage in the right cerebellar and pontine cavernomas with surrounding oedema and no evidence of obstructive hydrocephalus. These two cavernomas re-bled in a week duration causing episodes of incessant crying and irritability. After discussing the pros and cons of treatment, owing to stable clinical status, the patient is currently been managed conservatively.

Detection of anti-nuclear antibody by immunofluorescence assay and enzyme immunoassay in childhood systemic lupus erythematosus: experience from Bangladesh.

BACKGROUND: Systemic lupus erythematosus (SLE) is a multisystem, chronic but often episodic, autoimmune disease that is characterized by the presence of antinuclear antibodies (ANA). The criteria set by American College of Rheumatology are widely used for diagnosis of SLE. Elevation of ANA titer is the most sensitive of the ACR criteria. There are different methods for detection of ANA. Indirect immunofluorescence (ANA-IFA) and enzyme immunoassay (ANA-EIA) are commonly used methods. The sensitivity of ANA-IFA using HEp-2 cell substrate is 90-100% in systemic rheumatic diseases. In Bangladesh most of the laboratories use ANA-EIA for detection of ANA. As the sensitivity of ANA-EIA is lower than ANA-IFA it might be that we are missing many cases of ANA positivity in childhood SLE cases. OBJECTIVES: To detect ANA by immunofluorescence assay using HEp-2 cell substrate and enzyme immunoassay in childhood SLE and to compare the diagnostic performance of these methods. MATERIAL AND METHODS: This is a cross-sectional analytical study. A total of 40 patients were enrolled. Among them 20 were childhood SLE cases. Another 20 patients of childhood rheumatic diseases other than SLE were taken as the disease control group. RESULT: In childhood SLE cases, 100% were ANA-positive by IFA and 55% were ANA positive by EIA. The sensitivity of ANA-IFA was 100%. In contrast, sensitivity of ANA-EIA was 55%. CONCLUSION: ANA-IFA is superior to ANA-EIA for detection of ANA in childhood SLE patients. ANA-IFA should be the primary screening test for children with clinical features suggestive of SLE.