Association analysis of vitamin D receptor gene polymorphisms in North England population with Type 2 diabetes mellitus.

BACKGROUND: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens. AIM: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). Five hundred T2DM patients and 200 healthy subjects with normal HbA1c (≤ 5.0 %), fasting blood sugar (≤ 120 mg/dL) and random blood sugar (≤ 140 mg/dL) were enrolled. METHOLODGY: The genotypes were found by polymerase chain reaction restriction fragment length polymorphism and DNA sequencing. RESULTS: revealed that no considerable differences in frequencies of genotype and allele of the Bsm I and Fok I polymorphisms between healthy and patients in the North England (For Fok I: OR = 1.11, 95% CI: 0.72-1.12; for Bsm I: OR = 1.35, 95% CI: 0.79-1.98). CONCLUSION: It is recommended that both following polymorphisms of vitamin D receptor gene may not considerably add to the progression of T2DM in the North England.

Vitamin D receptor FokI polymorphism in a Pakistani population with type 2 diabetes mellitus.

Present research was undertaken with the aim to assess the association of VDR gene FokI polymorphism with T2DM in local population. The study comprised of 100 T2DM patients (DG) and 50 normal individuals (CG) groups. Demographic parameters; age, gender, BMI and blood pressure were recorded. Fasting glucose (FG), HbA1c, vitamin D, liver function parameters, renal function parameters and lipid profile were measured. Significantly higher (P<0.05) BMI (34.6±11.3 vs. 24.9±4.0kg/m2), sBP (141±16 vs. 124±14mm Hg), dBP (81±8 vs. 76±7mm Hg), FG (145±5.54 mg/dL vs. 80±3.55mg/dL, HbA1c (7.43±0.69 % vs. 4.85±0.33%) were evident in DG as compared to CG. Prominent reduction (P<0.05) in vitamin D levels (13.69±1.85mg/dL) manifested in case subjects than that of control subjects (22.36±2.34mg/dL) as a negative correlation existed between HbA1c and vitamin D. Compared to control participants, substantially different FokI allele distribution was observed in T2DM patients. Current study s also showed no significant link between FokI genotype and the biochemical parameters. Present study endorsed the fact that diabetic patients have hypovitaminosis D and variable VDR polymorphisms. However, confirmational studies are indecisive and warrants further research.

Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe

Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.

Extratos aquosos de rizomas Zingiber officinale foram estudados para avaliar os seus efeitos antidiabéticos em glicação de proteínas e sobre a difusão de glicose in vitro, no presente estudo. Extratos aquosos de Zingiber officinale foram examinados nas concentrações de 5, 10, 20 e 40 g extrato de planta/L. Os efeitos antidiabéticos observados eram dependentes da dose. O potencial antidiabético de Zingiber officinale se verificou, principalmente, através da inibição da difusão de glicose e, em menor extensão, através da redução da glicação. Estudos adicionais são necessários para elucidar se efeitos in vitro representam potencial terapêutico, restringindo a absorção de glicose pós-prandial e a glicação de proteínas plasmáticas em indivíduos diabéticos.