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1.
Proteomics ; 4(8): 2333-51, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15274127

ABSTRACT

We present an integrated proteomics platform designed for performing differential analyses. Since reproducible results are essential for comparative studies, we explain how we improved reproducibility at every step of our laboratory processes, e.g. by taking advantage of the powerful laboratory information management system we developed. The differential capacity of our platform is validated by detecting known markers in a real sample and by a spiking experiment. We introduce an innovative two-dimensional (2-D) plot for displaying identification results combined with chromatographic data. This 2-D plot is very convenient for detecting differential proteins. We also adapt standard multivariate statistical techniques to show that peptide identification scores can be used for reliable and sensitive differential studies. The interest of the protein separation approach we generally apply is justified by numerous statistics, complemented by a comparison with a simple shotgun analysis performed on a small volume sample. By introducing an automatic integration step after mass spectrometry data identification, we are able to search numerous databases systematically, including the human genome and expressed sequence tags. Finally, we explain how rigorous data processing can be combined with the work of human experts to set high quality standards, and hence obtain reliable (false positive < 0.35%) and nonredundant protein identifications.


Subject(s)
Body Fluids/chemistry , Gene Expression Profiling , Information Management/methods , Proteins/analysis , Proteins/chemistry , Proteomics/methods , Chromatography/instrumentation , Chromatography/methods , Computational Biology , Databases, Factual , Humans , Information Management/instrumentation , Mass Spectrometry/instrumentation , Mass Spectrometry/methods , Peptides/analysis , Proteins/genetics , Proteins/metabolism , Reproducibility of Results , User-Computer Interface
2.
Proteomics ; 4(7): 2125-50, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15221774

ABSTRACT

Human blood plasma is a useful source of proteins associated with both health and disease. Analysis of human blood plasma is a challenge due to the large number of peptides and proteins present and the very wide range of concentrations. In order to identify as many proteins as possible for subsequent comparative studies, we developed an industrial-scale (2.5 liter) approach involving sample pooling for the analysis of smaller proteins (M(r) generally < ca. 40 000 and some fragments of very large proteins). Plasma from healthy males was depleted of abundant proteins (albumin and IgG), then smaller proteins and polypeptides were separated into 12 960 fractions by chromatographic techniques. Analysis of proteins and polypeptides was performed by mass spectrometry prior to and after enzymatic digestion. Thousands of peptide identifications were made, permitting the identification of 502 different proteins and polypeptides from a single pool, 405 of which are listed here. The numbers refer to chromatographically separable polypeptide entities present prior to digestion. Combining results from studies with other plasma pools we have identified over 700 different proteins and polypeptides in plasma. Relatively low abundance proteins such as leptin and ghrelin and peptides such as bradykinin, all invisible to two-dimensional gel technology, were clearly identified. Proteins of interest were synthesized by chemical methods for bioassays. We believe that this is the first time that the small proteins in human blood plasma have been separated and analyzed so extensively.


Subject(s)
Blood Chemical Analysis/methods , Blood Proteins/metabolism , Plasma/metabolism , Proteomics/methods , Amino Acid Sequence , Chromatography , Chromatography, Gel , Chromatography, High Pressure Liquid , Chromatography, Ion Exchange , Computational Biology , Databases as Topic , Electrophoresis, Gel, Two-Dimensional/methods , Humans , Immunoglobulin G/chemistry , Mass Spectrometry , Molecular Sequence Data , Peptides/chemistry , Proteome , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Subcellular Fractions , Time Factors , Trypsin/pharmacology
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