ABSTRACT
The immune response to diphtheria toxin (clinically pronounced infection or carrier state with the presence of toxigenic bacteria) or toxoid (vaccination) the blood has been found to contain lymphocytes (1.00-5.71%), specifically binding diphtheria toxoid (DTX). The method for the determination of lymphocytes, specifically binding DTX, may serve both for early diagnosis and in the process of the disease, irrespective of the injection of therapeutic serum to the patient. The result of the test is obtained 2-3 days earlier than the positive result of the bacteriological analysis. The set of immune reagents for the rapid diagnosis of diphtheria by the method of rosette formation has been developed.
Subject(s)
Clinical Laboratory Techniques , Diphtheria/diagnosis , Emergencies , Antibodies, Bacterial/biosynthesis , Antigen-Antibody Reactions , Bacteriological Techniques , Diphtheria Toxoid/immunology , Humans , Lymphocytes/immunology , Rosette FormationABSTRACT
An experiment of rabbits subjected to artificial lead intoxication revealed that after the immunization of the animals with adsorbed DPT vaccine myelopid and thymoptin exhibited practically the same immunomodulating activity with respect to antibody response to diphtheria and tetanus toxoids, exceeding that of rabbits without intoxication. Still the immunomodulators used in this experiment did not completely compensate the negative effect of intoxication on antibody response to the toxoids. For the first time after immunization with adsorbed DPT toxoid lymphocytes capable of binding diphtheria toxoid were detected and the specific features of their dynamics in comparison with the activity of antitoxin were established. Myelopid and thymoptin were found to modulate immune response, evaluated by the content of antigen-binding lymphocytes, the injection of thymoptin completely compensating the negative effect of intoxication.
