ABSTRACT
The results of the examination of association of polymorphisms of DNA repair genes and chromosomal aberrations in lung cancer patients are discussed. A significant positive association between the hOGG1 G/G genotypes, XPD G/G genotype and lung cancer was found. The hOGG1 C/C genotypes were significantly negatively associated with lung cancer. The patient chromosomal aberration frequencies were significantly higher than in control. Carriers of all APE1 and XPD genotypes, XRCC1 G/G genotype, ADPRT T/T genotype, hOGG1 C/C and Ser/Cys genotypes had statistically significant differences in the level of the chromosomal aberrations between patient and control groups. Statistically significant differences in the level of chromosomal aberrations between XPD T/T and G/G genotype of lung cancer patients were observed.
Subject(s)
Adenocarcinoma/genetics , Chromosome Aberrations , DNA Repair/genetics , Lung Neoplasms/genetics , Adenocarcinoma/pathology , DNA Glycosylases/genetics , DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics , DNA-Binding Proteins/genetics , Genetic Association Studies , Genotype , Humans , Lung Neoplasms/pathology , X-ray Repair Cross Complementing Protein 1 , Xeroderma Pigmentosum Group D Protein/geneticsABSTRACT
The results of the investigation of the interrelationship between frequency of chromosomal aberrations and detoxification enzymes (GSTM1, GSTT1) and DNA repair (hOGG1, XPD) genes in the employees of fuel energy complex in Kemerovo are presented In the group of the workers frequency of metaphases with aberrations (3,9 +/- 0,2%: n = 288) was shown to be significantly higher than in the comparison group (2,1 0, 2%: n = +/- 141). In the group of workers and control donors statistically significant differences were revealed in the frequency of distribution of the GSTT1 and hOGG1 genes. The level of chromosomal aberrations was established to be higher in patients with GSTM1 genotype "0/0" in the group of control donors.
Subject(s)
Air Pollutants, Occupational/toxicity , Chromosome Aberrations/chemically induced , DNA Repair Enzymes/genetics , Polymorphism, Genetic , Xenobiotics/toxicity , Adult , Air Pollutants, Occupational/pharmacokinetics , Cells, Cultured , Chromosome Aberrations/statistics & numerical data , DNA Glycosylases/genetics , Glutathione Transferase/genetics , Humans , Inactivation, Metabolic , Power Plants/standards , Real-Time Polymerase Chain Reaction , Xenobiotics/pharmacokinetics , Xeroderma Pigmentosum Group D Protein/geneticsABSTRACT
The paper presents the results of investigating an association between the frequency of chromosome aberrations and the CYP1A1, CYP1A2*1F GSTM1, and GSTT1 genes involved in xenobiotic biotransformation in workers engaged in heat and power engineering. The group of workers is shown to have a significantly higher rate of metaphases with aberrations (4.03 +/- 0.17%; n = 231) than the control group (2.06 +/- 0.17%; n = 141). Cytogenetic disorders are not found to depend on gender, age, and length of service. The study indicated that the level of chromosome aberrations was significantly higher in carriers of the CTP1A1 C/C, GSTM1 0/0 and GSTT1 0/0 genotypes. In individuals with a combination of the GSTM1 0/0 and GSTT1 0/0 genotypes, the level of chromosome aberrations was ascertained to be significantly higher than that in those with the protective form of these genes.
Subject(s)
Chromosome Aberrations , Genome, Human , Polymorphism, Genetic , Power Plants , Workplace/standards , Adult , Biotransformation/genetics , Case-Control Studies , Chromosome Aberrations/chemically induced , Chromosome Aberrations/statistics & numerical data , Data Interpretation, Statistical , Genotype , Humans , Mutagens/pharmacokinetics , Mutagens/toxicity , Occupational Exposure/adverse effects , Xenobiotics/pharmacokinetics , Xenobiotics/toxicityABSTRACT
We present the results of cytogenetic monitoring of the districts in Kemerovo region, which differ in standardized indices of cancer incidence. It has been shown that residents of the districts with high incidence of malignancies had higher average frequency of metaphases with chromosomal aberrations than the control group (4.06 +/- 0.12% and 2.76 +/- 0.13%, respectively). This difference is caused primarily by single or paired fragments. The increase in the frequency of aberrant metaphase incidence in the districts with elevated cancer frequency was observed both in the male and female groups as well as both in adults and children.
Subject(s)
Chromosome Aberrations , Metaphase/genetics , Neoplasms/epidemiology , Neoplasms/genetics , Adult , Child , Child, Preschool , Female , Humans , Incidence , Male , SiberiaABSTRACT
Chromosomal aberration rate has been estimated in peripheral blood lymphocytes of subjects occupationally exposed to a set of hazardous factors (employees of the Kemerovo Heat Power Plant). The frequency of metaphases with aberrations in the workers (3.23 +/- 0.26%, N = 104) is significantly higher than in control subjects (2.11 +/- 0.28%, N = 70). The cytogenetic aberrations did not depend on the sex, age, duration of employment, or smoking.
Subject(s)
Chromosome Aberrations , DNA Damage , Occupational Exposure/adverse effects , Power Plants , Adult , Female , Humans , Lymphocytes , Male , Middle Aged , SiberiaABSTRACT
GSTM1 and GSTT1 gene polymorphisms were studied in Shorians, Teleuts, and Caucasians of the Kemerovo region. It has been shown that distribution of homozygous deletions in the examined groups is significantly heterogeneous. The frequency of deletion genotypes and combinations of deletion in these genes was lower in Shorians and, Teleuts than in Caucasians.
Subject(s)
Asian People , Glutathione Transferase/genetics , Polymorphism, Genetic , White People , Genotype , Homozygote , HumansABSTRACT
The authors present results of complex study concerning mutagenic and carcinogenic effects of work conditions in chemical-recovery industry. Oncologic morbidity and frequency of chromosomal aberrations in workers group is considerably higher than in controls. These effects appear to increase in males with longer length of service.
