ABSTRACT
A new two-staged method for identifying the rhesus appurtenance of the blood is proposed. During the first stage anti-D-super (IgM monoclonal anti-D-antibodies) are used, during the second stage rhesus appurtenance is identified in a tube using standard universal reagent for determining rhesus factor RhO(D) or it is done using gelatin test with Anti-D (IgG monoclonal anti-D-antibodies). A negative result at stage 1 and positive at stage 2 is a sign of partial antigen D, most probably of the VIth category. Subjects with partial D are qualified as rhesus-positive donors and rhesus-negative recipients.
Subject(s)
Blood Donors/classification , Rh-Hr Blood-Group System/immunology , Antibodies, Monoclonal , Erythroblastosis, Fetal/diagnosis , Female , Gelatin , Humans , Immunoglobulin G/immunology , Indicators and Reagents , Infant, Newborn , Male , Pregnancy , Rh-Hr Blood-Group System/classification , Rh-Hr Blood-Group System/geneticsABSTRACT
The article describes the new two-stage method of determining of Rh-factor belonging. At the first stage tsoliclon "Anti-D-super" (monoclonic anti-D-antibody class IgM) was used. At the second stage Rh-factor belonging is determined in test-tube, using standard universal reagent for definition Rh-factor RhO (D). Combination of negative results at the first stage with positive ones at the second stage is a sign of partial antigen D, most probably category VI. The persons with partial D are qualified as Rh-positive donors and Rh-negative recipients.
