ABSTRACT
Two Pseudomonas strains were isolated from the Ficus elastica leaves. The O-antigens were obtained using phenol-water method and mild acid degradation. The following structures of the O-polysaccharides were established by sugar analysis and 2D NMR spectroscopy: OPS of Pseudomonas psychrotolerans BIM B-1171 G -2)[aDGlcp(1-3)]bDRhap(1-3)aDManp(1-3)aDRhap(1- OPS of Pseudomonas sp. BIM B-1172 G -2)bDRhap(1-3)aDRhap(1-3)[aDGlcp(1-2)]aDRhap(1-.
Subject(s)
Ficus/chemistry , O Antigens/chemistry , Pseudomonas/chemistry , Ficus/microbiology , O Antigens/isolation & purification , Plant Leaves/chemistry , Plant Leaves/microbiology , Pseudomonas/isolation & purificationABSTRACT
Two Pseudomonas strains were isolated from the strawberry leaves. The O-antigens were obtained using phenol-water method and mild acid degradation. The following structures of the O-polysaccharides were established by sugar analysis and 2D NMR spectroscopy: OPS of Pseudomonas koreensis BIM B-970G â3)-α-D-FucNAcp-(1 â 2)-ß-D-Quip3NAc-(1 â 3)-α-L-6dTalp4OAc-(1â OPS of Pseudomonas oryzihabitans BIM B-1072G â4)-α-L-FucpNAm3OAc-(1 â 3)-α-D-QuipNAc-(1 â 4)-ß-D-GlcpNAc3NAcA-(1â Where Am - acetimidoyl.
Subject(s)
Fragaria/chemistry , Plant Leaves/chemistry , Polysaccharides/isolation & purification , Pseudomonas/chemistry , Carbohydrate Conformation , Magnetic Resonance Spectroscopy , Polysaccharides/chemistryABSTRACT
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSIâ3)-α-D-Manp-(1â2)-α-D-Manp-(1â; PSIIâ3)-α-D-Rhap-(1â2)-ß-D-Rhap-(1â3)-α-D-Rhap-(1â; α-D-Glcp-(1Ë©; 2.
Subject(s)
Polysaccharides, Bacterial/chemistry , Pseudomonas/chemistry , Carbohydrate Conformation , Nuclear Magnetic Resonance, BiomolecularABSTRACT
Two specific polysaccharides, together with an â4)-α-d-Glcp-(1â glucan (bacterial glycogen), were obtained from a lipopolysaccharide preparation isolated from the bacterium Pseudomonas putida BIM B-1100 by phenol/water extraction. The following structures of the polysaccharides were established by composition analysis, Smith degradation, ESI-MS, and 1D and 2D NMR spectroscopy.
