[The alteration of concentration of essential microelements in blood plasma in children with chronic diseases of gastrointestinal tract and having different body mass].

The article deals with the characteristics of alterations of mineral metabolism indicators in children of various age with chronic diseases ofgastrointestinal tract and having body mass of normal and different surplus degrees. The sample consisted of 127 children aged from 6 to 15 years with chronic gastroduodenitis, pancreatitis and cholecystitis. The concentration of bioelements in plasma was determined using the technique of mass spectrometry with inductively coupled plasma. The study established the increase of concentration of molybdenum 4.3 times, chrome--8.4 times and selenium--1.36 times. The revealed bioelemental misbalance impacts the overall health of children and course of disease and hence has to be accounted in case of application of treatment targeted to the correction of mineral metabolism.

[Analytical methods for measuring the content of trace elements in children with chronic gastrointestinal diseases].

Plasma trace elements (TE) were determined by atomic absorption spectrometry with electrothermal atomization and by mass spectrometry with inductively bound plasma. These techniques are highly effective and may be used to determine TE in biological samples. In children, chronic gastrointestinal diseases are accompanied by mineral metabolic disorders. In children with chronic gastrointestinal diseases whose age was 6 to 15 years and whose weight was varying, the scores of some TEs depend on a child's body weight and may be used to evaluate the degree of a pathological process.

Disorders in restructuring of T-cell receptor gamma-chain in malignant skin lymphomas.

Disorders in restructuring of T-cell receptor gamma-chain in DNA from skin biopsy specimens and peripheral blood lymphocyte of patients with malignant skin lymphomas at different stages of the disease were detected by PCR. Nucleotide sequences of monoclonal T-cell receptors were determined. The study showed that DNA sequences from the skin and lymphocytes did not coincide in some cases.

Relative content of cytokines in different tissues in mycosis fungoides.

The concentrations of tumor necrosis factor-alpha, interleukin-1beta, interleukin-2, interleukin-4, and the content of antibodies to H1 histone fraction in peripheral blood lymphocytes, epidermis, and serum were evaluated in patients with mycosis fungoides. It was found that as the disease progresses, cytokine synthesis is switched from Th1 to Th2 class and production of proinflammatory cytokines increases, which indirectly promotes the development of the autoimmune reaction.

[Chromatin nuclear proteins in evaluation of the effectiveness of treatment of patients with psoriasis]. / Iadernye belki khromatina v otsenke éffektivnosti lecheniia bol'nykh psoriazom.

Histone proteins and nucleic acid precursors were studied in 10 normal subjects and 20 patients with disseminated psoriasis before and after combined therapy including Enkad. A drop in the concentrations of H1 histones was detected in the blood lymphocytes and plasma in parallel with an increase in the concentrations of arginine-rich histones and a many-fold decrease in the acid-soluble fraction representing a pool of nucleic acid precursors. After combined therapy the ratio of lysin- and arginine-rich nuclear proteins in blood lymphocytes changed significantly and approximated the normal value; the distribution of histone-like proteins virtually did not change in the plasma, but the level of plasma and erythrocyte acid-soluble fraction normalized. This indicates positive changes in nucleic acid transport after therapy. The authors demonstrate the possibility of these findings for evaluation of the treatment efficiency.

[Effects of vitamin D metabolites on indicators of protein, phosphorus and calcium metabolism in children with chronic renal failure]. / Vliianie metabolitov vitamina D na pokazateli belkovogo i fosforno-kal'tsevogo obmena u detei s khronicheskoi pochechnoi nedostatochnost'iu.

Amino acid analysis and investigation of nitrogen, calcium and phosphorus balance were done in two groups of patients with renal failure and osteodystrophy on vitamin D metabolites and analogues treatment. The results of the investigation confirm vitamin D metabolites influence on free amino acid metabolism and on nitrogen, calcium, phosphorus balance. We observed significant decreases of blood amino acids and good influence on renal osteodystrophy in patients treated with vitamin D metabolites. Vitamin D metabolites didn't influence retention, urinary and fecal excretion of endogenous nitrogen in patients with renal failure.

[Effect of vitamin D metabolites on the markers of protein, calcium and phosphorus turnover in children with chronic renal failure]. / Vliianie metabolitov vitamina D na pokazatelibelkovogo i fosforno- kal'tsievogo obmena u detei s khronicheskoi pochechnoi nedostatochnost'iu.

Amino acid analysis and investigation of nitrogen, calcium and phosphorus balance were done in two groups of the patients with renal failure and osteodystrophy on the Vitamin D metabolites and analogues treatment. The results of the investigation confirm vitamin D metabolites influence on free amino acid turnover and on nitrogen, calcium, phosphorus balance. We observed significant decreases of blood amino acids and good influence on renal osteodystrophy in patients treated with the vitamin D metabolites. Vitamin D metabolites didn't influence on the retention, urinary and fecal excretion of endogenous nitrogen in patients with renal failure.

[Lupus erythematosus: effects of different therapeutic methods on intranuclear characteristics of lymphoid cells]. / Vliianie razlichnykh metodov lecheniia krasnoi volchanki na vnutriiadernye kharakteristiki limfoidnykh kletok.

In lymphocytes of peripheral blood from 46 patients with various forms of lupus erythematosus measurements were made of histone fractions and DNA. It is proved that intranuclear characteristics of lymphoid cells can be used in the choice of treatment and assessment of its efficacy. Lupus erythematosus treatment with glucocorticoids (diprospan, diprospan + celeston) is effective in correction of intranuclear lymphocyte changes in this disease.

[An analysis of the lymphoid cell histones in patients with different forms of lupus erythematosus]. / Analiz gistonov limfoidnykh kletok u bol'nykh s razlichnymi formami krasnoi volchanki.

The study was made of lisine- and arginine-rich histone fractions and DNA in lymphocytes of peripheral blood in patients with lupus erythematosus. The findings showed intranuclear changes of immunocompetent cells in various forms of the disease. The above parameters may be used in the diagnosis, evaluation of treatment efficacy, prognosis.

[Effects of vitamin D on protein turnover in children with renal osteopathy]. / Vliianie vitamina D na pokazateli belkovogo obmena u detei s renal'nymi osteopatiiami.

Amino acid analysis and investigation of nitrogen balance were done in 2 groups of the patients with renal failure and osteodystrophy on the diet and vitamin D treatment. The results of the investigations confirm vitamin D influence on free amino acid turnover. We observed significant elevations of plasma amino acids in patients treated with the diet and vitamin D in comparison with the patients without vitamin D supplementation. Vitamin D didn't influence on the retention, urinary and fecal excretion of endogenous nitrogen in patients with renal failure

[The amino acid spectrum of plasma and erythrocytes in children with kidney diseases at the stage of chronic kidney insufficiency]. / Aminokislotnyi spektr plazmy i éritrotsitov pri bolezniakh pochek v stadii khronicheskoi pochechnoi nedostatochnosti u detei.

The effect of diets with varying protein restrictions on the amino-acid spectrum of plasma and erythrocytes was studied in 46 children with total chronic renal insufficiency (CRI), stages I and II, resultant from tubulo-interstitial diseases of the kidneys and glomerulonephritis. Qualitative and quantitative changes in the spectrum of blood amino acids are recorded at the initial stage of CRI and depend on the degree of protein restriction in the diet. The diet with a sharp protein restriction (Giordano-Giovanetti type) produces hypoazotemic effect and possesses anabolic properties. Disorders in amino-acid metabolism serve indications for using essential amino acids and their ketoanalogues in children with uremia.

[Metabolism of nuclear proteins of the thymus and bone marrow cells in mice with hepatoma 22]. / Metabolizm iadernykh belkov v kletkakh timusa i kostnogo mozga myshei s gepatomoi 22.

Specific characteristics of nuclear proteins synthesis were studied in thymus and bone marrow cells of mice with solid hepatoma 22, which enabled to find three main periods of alterations in the protein synthesis within 1-3 days, 5-9 and 11-13 days after administration of hepatoma 22. The most distinct alterations were observed within first day after the hepatoma 22 administration, which may be considered as a primary reaction of nuclear apparatus in the cells of tumor-bearing animals.

[Free amino acids in the blood serum of rats with liver failure developing as a consequence of the functioning of small intestine fistulae]. / Svobodnye aminokisloty v syvorotke krovi u krys s pechenochnoi nedostatochnost'iu, razvivaiushcheisia vsledstvie funktsionirovaniia tonkokishechnykh svishchei.

The experiments on 60 mongrel rats with hepatic failure due to small intestinal fistulas of high and medium location were carried out for the examination of amino acid spectrum in the animal plasma. 16 free amino acids were studied by ion-exchange chromatography. It has been established that hepatic failure due to small intestinal fistulas is characterized by the reduction of the total amino acid content by 20-30%. These changes were brought about mainly by the reduction of glucoblastic and essential amino acids. A dramatic (400%) increase in His and a slight (20%) increase in Asp were noted in high fistulas. The changes were caused by profound losses through fistulas which markedly activated gluconeogenesis and led to full exhaustion of amino acid reserves. It was concluded that the question of diagnostic amino acid value should be considered taking into account the amount of losses and the activity of gluconeogenesis in the pathogenesis of hepatic failure.

[Characteristics of the levels of various nucleic acid precursors in the tissues of rabbits with various thyroid conditions]. / Osobennosti soderzhaniia nekotorykh predshestvennikov nukleinovykh kislot v tkaniakh krolikov s razlichnym tireoidnym sostoianiem.

The content of the precursors and metabolites of nucleic acids in the brain and liver of normal rabbits, rabbits with hypothyrosis (thyroidectomy) and hyperthyrosis (100-150 Mg of L-thyroxine per 1 kg of body mass daily for 10-12 days) was determined by liquid chromatography with a high resolution. A decrease in the content of deoxyguanosine, thymine, hypoxanthine, cytosine, thymidine and an increase in the content of inosine, adenine and adenosine in the liver of animals with hypothyrosis were shown. In the brain of these rabbits the level of cytidine was lowered, and the levels of deoxyguanosine, thymine, inosine, hypoxanthine, adenine and uracil were on an increase. An increase in the levels of deoxyguanosine, thymine, adenine, thymidine and a decrease in the levels of cytidine, hypoxanthine, cytosine, adenine and uracil in the liver were noted in hyperthyrosis. In the brain of rabbits with hyperthyrosis an increase in the levels of deoxyguanosine, cytidine, inosine and adenine was combined with a decrease in the levels of thymine and hypoxanthine. These thyroid-dependent changes in the content of registered metabolites and precursors of nucleic acids in tissues suggest that the observed shifts illustrate one of many mechanisms of the regulation of cell metabolism by thyroid hormones.

[Effect of hormones and partial hepatectomy on incorporation of labeled precursor in nuclear proteins of mouse liver]. / Vliianie gormonov i operatsii chastichnoi gepatéktomii na vkliuchenie mechenogo predshestvennika v iadernye belki pecheni myshei.

Partial hepatectomy altered the nuclear protein synthesis, most distinct for H1 histones, within 1,5-3 hrs after the operation. Laparotomy, administration of hormones and dibutyryl-cAMP did not affect the cell nuclear apparatus throughout similar to partial hepatectomy. The cell response to partial hepatectomy appears to develop involving a number of factors between which unspecific stress reactions and hormones carry out a definite role.

[Effect of a single dose of hydrocortisone and adrenaline on the synthesis of nuclear proteins and DNA in the rat thymus]. / Vliianie odnokratnogo vvedeniia gidrokortizona i adrenalina na uroven' sinteza iadernykh belkov i DNK v timuse krys.

Single injections of hydrocortisone and adrenaline low doses long before the rat killing induced a change in the level of thymic DNA and nuclear protein synthesis. The effect and direction of hormone activity were dependent on the day time and duration of hormone presence in the organism. Hydrocortisone in a dose of 10 mg/kg, injected at 11 o'clock, promoted thymic DNA synthesis, whereas adrenaline in a dose of 0.06 mg/kg, administered at 11 o'clock, inhibited DNA synthesis, stimulating it, when injected at 23 o'clock.