[Techniques for detection and role of extracellular neutrophil traps in antimicrobial protection].

New methods are proposed for the measurement of the number of extracellular neutrophil traps in peripheral blood and mucosal secretion. Effect of microbial factors on the ability ofgranulocytes to release nuclear DNA into extracellular space and form extracellular traps is evaluated. Normal microflora species are shown to more actively stimulate build-up of extracellular network. Formation of extracellular neutrophil traps may be a major efficacious mechanism for mucosal antimicrobial protection.

[Influence of Solcotrichovac vaccine on formation of neutrophil extracellular traps].

AIM: To study the influence of Solcotichovac vaccine on formation of neutrophil extracellular traps. MATERIALS AND METHODS: Solcotrichovac vaccine administered in various doses was used in order to assess the formation of neutrophil extracellular traps in peripheral blood of women of childbearing age. RESULTS: Therapeutic dose of Solcotrichovac stimulated the most prominent formation of neutrophil extracellular traps. CONCLUSION: Solcotrichovac vaccine stimulated non-specific arm of mucosal immunity due to formation of neutrophil extracellular traps and it is probable that this mechanism explains the effect of this vaccine.

[Immunological indices in differential diagnosis of active and inactive chronic endocervicitis].

A total of 132 patients were examined for chronical endocervicitis by clinical, immunological and morphological methods. According to the results obtained the patients were subdivided into several groups. The first group covered 77 females with an active clinical somatic form of the disease. The second group included 55 patients with chronical inactive endocervicitis. Controls included 25 practically healthy females. No differences were found between immunological indices of peripheral blood in groups of patients with active and inactive forms of chronic endocervicitis. Immunological tests on cervical mucus in patients with chronic endocervicitis revealed differences in the total number of leukocytes, content of living cells, functional activity of neutrophils, concentration of immunoglobulins and proinflammatory cytokines. This study may contribute to the development of immunological criteria for diagnosis of inflammatory process activity as well as a diagnostic model.

[Anti-inflammatory cytokines of cervical secretions and the blood serum in women with genital infections]. / Protivovospalitel'nye tsitotoksiny tservikal'nogo sekreta i syvorotki krovi u zhenshchin s genital'noi infektsiei.

In cervical secretions of healthy non-pregnant women of the reproductive age high concentrations of ant-inflammatory cytokines, greatly exceeding those in blood serum, were detected. During pregnancy the level of TNF-alpha in cervical secretions dropped. The inflammation of the uterus neck was accompanied by a drop in the levels of IL-alpha and IL-1beta and a rise in the level of IL-8 in cervical secretions in pregnant and non-pregnant women. Similar changes in the cytokine profile occurred also in the blood serum, but they were less pronounced and could be observed only in non-pregnant women. The threat of the interruption of pregnancy, developing simultaneously with cervicitis, was accompanied not only by changes in the levels of cytokines in cervical secretions, but also by a perceptible increase in the content of IL-1alpha in the blood serum.