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1.
Ocul Immunol Inflamm ; 29(2): 244-249, 2021 Feb 17.
Article in English | MEDLINE | ID: mdl-32141793

ABSTRACT

Purpose: Identify genes associated with ocular sarcoidosis (OS).Methods: We genotyped 1.1 million genetic variants to identify significant OS associations, defined as those that achieved p < 5 × 10-8 in a genome-wide comparison of OS cases to healthy controls in our European- or African-American cohorts (EA, AA). Potential functional roles of all associated variants were assessed.Results: Eight significant non-HLA variants were found in AA OS cases compared to healthy controls and confirmed as at least suggestive when comparing OS to non-OS cases. Seven of these were within MAGI1 and include transcription factor binding sites and expression quantitative trait loci. Our EA cohort, while showing similar effect sizes at variants within MAGI1, had no significant variants. Association analysis of HLA-DRB1 alleles confirmed association to OS in EA to *04:01.Conclusion: Our results support organ-specific genetic risk in OS in a compelling candidate, MAGI1, known to be associated with barrier function and autoimmunity.


Subject(s)
Adaptor Proteins, Signal Transducing/genetics , Black or African American/genetics , Cell Adhesion Molecules/genetics , Eye Diseases/genetics , Genome-Wide Association Study/methods , Guanylate Kinases/genetics , HLA-DRB1 Chains/genetics , Polymorphism, Single Nucleotide , Sarcoidosis/genetics , Adaptor Proteins, Signal Transducing/metabolism , Adult , Autoimmunity/genetics , Case-Control Studies , Cell Adhesion Molecules/metabolism , DNA/genetics , Eye Diseases/ethnology , Eye Diseases/immunology , Female , Follow-Up Studies , Genotype , Guanylate Kinases/metabolism , HLA-DRB1 Chains/immunology , Humans , Male , Middle Aged , Morbidity/trends , Sarcoidosis/ethnology , Sarcoidosis/immunology , United States/epidemiology
2.
Front Immunol ; 11: 567342, 2020.
Article in English | MEDLINE | ID: mdl-33363531

ABSTRACT

Sarcoidosis is a systemic inflammatory disease characterized by infiltration of immune cells into granulomas. Previous gene expression studies using heterogeneous cell mixtures lack insight into cell-type-specific immune dysregulation. We performed the first single-cell RNA-sequencing study of sarcoidosis in peripheral immune cells in 48 patients and controls. Following unbiased clustering, differentially expressed genes were identified for 18 cell types and bioinformatically assessed for function and pathway enrichment. Our results reveal persistent activation of circulating classical monocytes with subsequent upregulation of trafficking molecules. Specifically, classical monocytes upregulated distinct markers of activation including adhesion molecules, pattern recognition receptors, and chemokine receptors, as well as enrichment of immunoregulatory pathways HMGB1, mTOR, and ephrin receptor signaling. Predictive modeling implicated TGFß and mTOR signaling as drivers of persistent monocyte activation. Additionally, sarcoidosis T cell subsets displayed patterns of dysregulation. CD4 naïve T cells were enriched for markers of apoptosis and Th17/Treg differentiation, while effector T cells showed enrichment of anergy-related pathways. Differentially expressed genes in regulatory T cells suggested dysfunctional p53, cell death, and TNFR2 signaling. Using more sensitive technology and more precise units of measure, we identify cell-type specific, novel inflammatory and regulatory pathways. Based on our findings, we suggest a novel model involving four convergent arms of dysregulation: persistent hyperactivation of innate and adaptive immunity via classical monocytes and CD4 naïve T cells, regulatory T cell dysfunction, and effector T cell anergy. We further our understanding of the immunopathology of sarcoidosis and point to novel therapeutic targets.


Subject(s)
Gene Expression Profiling , Monocytes/immunology , Monocytes/metabolism , Sarcoidosis/etiology , Single-Cell Analysis , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Transcriptome , Apoptosis/genetics , Apoptosis/immunology , Biomarkers , Case-Control Studies , Cell Movement/genetics , Cell Movement/immunology , Clonal Anergy/genetics , Clonal Anergy/immunology , Disease Progression , Female , Humans , Immunophenotyping , Male , Models, Biological , Organ Specificity , Receptors, Antigen, T-Cell/metabolism , Sarcoidosis/diagnosis , Sarcoidosis/metabolism , Sarcoidosis/therapy , Signal Transduction
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