ABSTRACT
OBJECTIVE: The aim of this study was to evaluate quantitative telomerase activity in malignant, benign and normal gynecological tissue samples by using the Telomerase-PCR ELISA kit, and to determine a cut-off level for malignancy by this quantitative method. MATERIALS AND METHODS: Fifty gynecological tumors, 27 benign gynecological disorders and 29 normal tissues were analyzed by the Telomerase-PCR ELISA kit. All tissues were confirmed by a pathologist. A ROC (receiver operator characteristic) curve was drawn to determine a threshold level best discriminating malignant tissues from benign pathologies and normal tissues. Telomerase activity was compared in malignant, benign and normal tissues. RESULTS: The mean level of telomerase activity of the malignant tumor samples (1.03 +/- 0.53 units) was significantly (p < .001) higher than the normal tissues (0.13 +/- 0.07 units) and benign pathologies (0.37 +/- 0.25 units). The cut-off point to differentiate malignant samples from benign samples was set at 0.42 units, where the sensitivity was 93.8% and the specificity was 89.3%. Positive predictive value was 84% and negative predictive value was 89.3%. There was a significant difference in telomerase activity between malignant, benign and normal tissues within each histological group. CONCLUSION: In this preliminary study, the telomerase-PCR ELISA method was found to have a high sensitivity and specificity to differentiate malignant gynecological tissues from benign tissues.
Subject(s)
Biomarkers, Tumor/metabolism , Genital Neoplasms, Female/metabolism , Telomerase/metabolism , Carcinoma, Endometrioid/metabolism , Carcinoma, Endometrioid/pathology , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/pathology , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Female , Genital Neoplasms, Female/pathology , Humans , Polymerase Chain Reaction , Predictive Value of Tests , ROC Curve , Sensitivity and SpecificityABSTRACT
Acute fatty liver is a rare but fatal complication of pregnancy. Here we describe a patient presenting with stupor and jaundice after aspirin intake at 35 weeks of gestation. Supportive management and delivery resulted in uneventful discharge of the patient and the newborn. Differential diagnosis and management of this condition are discussed.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/adverse effects , Aspirin/adverse effects , Fatty Liver/chemically induced , Pregnancy Complications/chemically induced , Adult , Aspirin/administration & dosage , Blood Component Transfusion , Diagnosis, Differential , Fatty Liver/mortality , Fatty Liver/therapy , Female , Humans , Pregnancy , Pregnancy Complications/diagnosis , Pregnancy Complications/therapy , Pregnancy Trimester, ThirdABSTRACT
A fetal sublingual cystic lesion was diagnosed by routine prenatal ultrasonography at 21 weeks of gestation and followed up until term in a tertiary care center. Fetal growth was normal as was the amniotic fluid volume. Ex utero intrapartum treatment was performed and the cyst was aspirated to allow breathing and swallowing during planned Cesarean section. The cyst was totally excised when the newborn was 27 days of age and histological examination revealed a mucous cyst of the mouth floor.
Subject(s)
Fetal Diseases/diagnostic imaging , Ranula/diagnostic imaging , Ultrasonography, Prenatal , Adult , Female , Gestational Age , Humans , Infant , Mouth Mucosa , Pregnancy , Pregnancy Trimester, Second , Ranula/congenital , Ranula/surgeryABSTRACT
Prenatal diagnosis of congenital atresia of the larynx is difficult but is possible by the findings of increased lung echogenicity and size coexisting with fetal ascites in ultrasonography. Sonographic findings may not always be typical. We report on a case of congenital laryngeal atresia diagnosed prenatally by the findings of fetal hydrops and hyperechogenic lungs. Our case presented with oligohydramnios. We also review syndromes that demonstrate laryngeal anomalies.
Subject(s)
Laryngostenosis/diagnostic imaging , Larynx/abnormalities , Ultrasonography, Prenatal , Abortion, Eugenic , Adult , Cystic Adenomatoid Malformation of Lung, Congenital/diagnostic imaging , Diagnosis, Differential , Female , Gestational Age , Humans , Laryngostenosis/congenital , Laryngostenosis/pathology , Larynx/diagnostic imaging , Male , PregnancySubject(s)
Cytomegalovirus Infections/complications , Cytomegalovirus/isolation & purification , Hydrops Fetalis/diagnosis , Pregnancy Complications, Infectious , Prenatal Diagnosis , Adult , Cytomegalovirus/genetics , DNA, Viral/isolation & purification , Female , Humans , Hydrops Fetalis/etiology , PregnancyABSTRACT
The ovary is a frequent site of metastasis from a wide variety of malignant neoplasias, with the majority originating in the GI tract. The best known tumor of this type is signet ring cell adenocarcinoma (Krukenberg tumor). The gallbladder and bile ducts are rare sources of these metastases. We are reporting two such cases in which the patients presented with no hepatic symptoms and vague gastrointestinal complaints. The gallbladder and bile duct carcinomas were incidental findings during exploratory laparatomy for an ovarian mass.
