ABSTRACT
The new kit of reagents in format of the immunochip "ImmunoChip Borreliosis" for multiplex serologic analysis of mite-borne borreliosis demonstrated high diagnostic sensitivity and specificity. The percentage of detection of specific immunoglobulins was higher in "ImmunoChip Borreliosis" as compared with screening results in immune enzyme analysis. The high correlation between results of testing in immunochip and data of immune blotting is demonstrated to.
Subject(s)
Borrelia burgdorferi/isolation & purification , Ixodes/microbiology , Lyme Disease/diagnosis , Lyme Disease/microbiology , Animals , Antibodies, Bacterial/blood , Antibodies, Bacterial/immunology , Antibodies, Bacterial/isolation & purification , Borrelia burgdorferi/pathogenicity , Humans , Immunoglobulin G/blood , Immunoglobulin G/isolation & purification , Immunoglobulin M/blood , Immunoglobulin M/isolation & purification , Lyme Disease/blood , Lyme Disease/immunology , Sensitivity and Specificity , Serologic Tests/methodsABSTRACT
Peptide CH3CO-Lys-Lys-Arg-Arg-NH2 (protectin) was synthesized and its activity was studied on the model of experimental myocardial infarction in rats in comparison to the reference antihypoxant drug riboxin. Intranasal injections ofprotectin at doses within 2-20 microg/kg once a day by course of 7 days produced a pronounced anti-ischemic action, improved coronary circulation of the blood, increases contractile activity of myocardium, reduced intensity of lipid peroxidation, and improved antioxidant protection. In some respects (improved coronary circulation of the blood, increased antioxidant protection), protectin was more effective than riboxin.
Subject(s)
Inosine Diphosphate/administration & dosage , Myocardial Infarction/drug therapy , Oligopeptides , Administration, Intranasal , Animals , Antioxidants/metabolism , Coronary Circulation/drug effects , Disease Models, Animal , Dose-Response Relationship, Drug , Lipid Peroxidation/drug effects , Male , Oligopeptides/administration & dosage , Oligopeptides/chemical synthesis , Rats , Rats, Sprague-DawleyABSTRACT
The activity of the KKRR synthetic peptide corresponding to the 15-18 sequence of human adrenocorticotropic hormone (ACTH) and its analogues KKKK, RRRR, RRKK, kKRR, KkRR, KKrR, and KKRr (amino acid residues of the D configuration are designated by small letters) was studied in vivo on rats under cold and heat shock. Intranasal administration of the KKRR peptide at doses of 2-10 microg/animal 1 day before the shock was found to prevent a dramatic increase in the level of corticosterone in rat adrenal glands and blood plasma caused by the temperature effect. Amino acid substitutions in the KKRR peptide were shown to result in an abrupt decrease in its activity. The peptide analogues exhibit a low stress-protective activity and had a low affinity for the ACTH receptor.
