ABSTRACT
A strategy for determining the bioaccessibility of bromine and iodine from edible seaweeds was proposed for the first time using microwave-induced combustion (MIC) and ion chromatography coupled to mass spectrometry (IC-MS) after in vitro digestion. The concentrations of bromine and iodine in edible seaweeds using the proposed methods (MIC and IC-MS) were not statistically different from those using MIC and inductively coupled plasma mass spectrometry (p > 0.05). Trueness was assessed by recovery experiments (101-110%, relative standard deviation <10%). Following an in vitro digestion protocol, MIC was proposed as sample preparation for bioaccessible and residual fractions. Using this strategy, the mass balance totaled from 97 to 111%. No statistical difference (p > 0.05) was observed between the total concentration of bromine or iodine and their concentration in bioaccessible and residual fractions for three edible seaweed species, indicating full analyte quantification in the fractions.
ABSTRACT
The halogen determination is important in view of their biological and environmental roles, but their determination has still been considered a challenge, especially at low concentrations. Therefore, a method for honey decomposition using microwave-induced combustion (MIC) combined with ion chromatography and conductimetric detection (for Cl, F, and S determination) or mass detection (for Br and I determination) (IC-CD-MS) is proposed. Trueness was evaluated by adding reference materials (RMs) or a standard solution in the sample. By using 50 mmol L-1 NH4OH as the absorbing solution, recoveries for all analytes were between 94 and 103%, in both tests. Moreover, no statistical difference (t test, confidence level of 95%) was observed for the results obtained by IC in comparison with those obtained by inductively coupled plasma optical emission spectroscopy (Cl and S) and by inductively coupled plasma mass spectrometry (Br and I). Finally, the proposed method was applied to 19 honey samples from different origins. The concentrations ranged from < 0.45 to 2.39 mg kg-1 (Br), 21.8 to 671 mg kg-1 (Cl), and 11 to 154 mg kg-1 (S), while the F and I concentrations were below that their quantification limits (LOQs) in all analyzed samples. The LOQs for Br, Cl, F, I, and S were 0.45, 21, 3.7, 0.077, and 8.7 mg kg-1, respectively. The MIC method provided a compatible solution to IC for the halogen and S determination in honey by a single analysis. Graphical abstract.
