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Appl Radiat Isot ; 79: 103-8, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23747514

ABSTRACT

Cytogenetic and somatic effects of various x-ray treatments were evaluated in pepper, Capsicum baccatum var. pendulum cv. "Cayenne", with the aim to assess optimal conditions for obtaining viable lines. The cytogenetic effects were quantified by counting chromosome aberrations. The level of DNA fragmentation was estimated with TUNEL test (terminal transferase mediated dUTP-fluorescein nick end labeling). Irradiation to 20 Gy with 16-h presoaking can be a suitable treatment of the selected pepper cultivar for a mutagenesis program.


Subject(s)
Capsicum/radiation effects , Radiation, Ionizing , Capsicum/genetics , Chromosome Aberrations , DNA Fragmentation , In Situ Nick-End Labeling
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