Next-generation methods for early disease detection in crops.

Plant pathogens are commonly identified in the field by the typical disease symptoms that they can cause. The efficient early detection and identification of pathogens are essential procedures to adopt effective management practices that reduce or prevent their spread in order to mitigate the negative impacts of the disease. In this review, the traditional and innovative methods for early detection of the plant pathogens highlighting their major advantages and limitations are presented and discussed. Traditional techniques of diagnosis used for plant pathogen identification are focused typically on the DNA, RNA (when molecular methods), and proteins or peptides (when serological methods) of the pathogens. Serological methods based on mainly enzyme-linked immunosorbent assay (ELISA) are the most common method used for pathogen detection due to their high-throughput potential and low cost. This technique is not particularly reliable and sufficiently sensitive for many pathogens detection during the asymptomatic stage of infection. For non-cultivable pathogens in the laboratory, nucleic acid-based technology is the best choice for consistent pathogen detection or identification. Lateral flow systems are innovative tools that allow fast and accurate results even in field conditions, but they have sensitivity issues to be overcome. PCR assays performed on last-generation portable thermocyclers may provide rapid detection results in situ. The advent of portable instruments can speed pathogen detection, reduce commercial costs, and potentially revolutionize plant pathology. This review provides information on current methodologies and procedures for the effective detection of different plant pathogens. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Phosphorus recovery from agricultural waste via cactus pear biomass.

In this study, the potential of cactus pear pruning waste (CPPW) as a low-cost adsorbent biomass for phosphorus (P) removal from aqueous solutions was investigated in batch mode. Biomass samples derived from cactus pear were collected and analyzed to investigate their properties when enriched with either calcium (Ca) or iron (Fe). The examination focused on the capacity of these samples to remove P. The P removal capacities were determined to be 2.27 mg g-1, 1.33 mg g-1, and 1.87 mg g-1 for Ca2+-enriched, Fe2+-loaded, and Fe3+-loaded biomass respectively. Among the various models studied, the Langmuir isotherm model was identified as the most appropriate for accurately describing the P adsorption the enriched biomass. The kinetics of the adsorption process were analyzed by applying the pseudo-first-order, pseudo-second-order, and intraparticle diffusion models. The pseudo-second-order model provided the best fit to the experimental data. Furthermore, the desorption and regeneration process was investigated, revealing minimal P desorption (less than 8%) from Ca or Fe-loaded biomass, indicating the strong stability of the biomass-cation-P system. The estimated cost ranged from 8 to 161 euros per tonne, with an additional 230 euros when considering the pruning costs inherent to the crop. These costs fall below the threshold (320 euros per tonne) for the economically viable P reuse at the farm level. Consequently, CPPW, when reduced to powder and loaded with ions, emerges as an affordable adsorbent with good removal performance, offering a promising avenue for direct utilization in agriculture as both soil conditioner and fertiliser.

The unseen world beneath our feet: Heliyon soil science. Exploring the cutting-edge techniques and ambitious goals of modern soil science.

In the face of climate change, ecosystem destruction, desertification, and increasing food demand, soil conservation is crucial for ensuring the sustainability of life on Earth. The Soil Section of Heliyon aims to be a platform for basic and applied soil science research, emphasizing the central role of soils and their interactions with human activities. This editorial highlights recent research trends in soil science, including the evolving definition of soil, the multifunctionality of soils and their biodiversity, soil degradation and erosion, the role of soil microflora, advancements in soil mapping techniques, global change and the carbon cycle, soil health, the relationship between soil and buildings, and the importance of considering soil quality in land use planning and policies. The Heliyon Soil Science section seeks to publish scientifically accurate and valuable research that explores the diverse functions of soil and their significance in sustainable land-use systems.

The crucial interactions between climate and soil.

Since the birth of soil science, climate has been recognized as a soil-forming factor, along with parent rock, time, topography, and organisms (from which humans were later kept distinct), often prevalent on the other factors on the very long term. But the climate is in turns affected by soils and their management. This paper describes the interrelationships between climate - and its current change - and soil, focusing on each single factor of its formation. Parent material governs, primarily through the particle size distribution, the capacity of soil to retain water and organic matter, which are two main soil-related drivers of the climate. Time is the only unmanageable soil-forming factor; however, extreme climatic phenomena can upset the soil or even dismantle it, so as to slow down the pathway of pedogenesis or even make it start from scratch. Topography, which drives the pedogenesis mostly controlling rainfall distribution - with repercussions also on the climate - is not anymore a given factor because humans have often become a shaper of it. Indeed humans now play a key role in affecting in a plethora of ways those soil properties that most deal with climate. The abundance and diversity of the other organisms are generally positive to soil quality and as a buffer for climate, but there are troubling evidences that climate change is decreasing soil biodiversity. The corpus of researches on mutual feedback between climate and soil has essentially demonstrated that the best soil management in terms of climate change mitigation must aim at promoting vegetation growth and maximizing soil organic matter content and water retention. Some ongoing virtuous initiatives (e.g., the Great Green Wall of Africa) and farming systems (e.g., the conservation agriculture) should be extended as much as possible worldwide to enable the soil to make the greatest contribution to climate change mitigation.

Outlook from the soil perspective of urban expansion and food security.

The use of soil as support for built-up areas represents only one of its several functions. Farmlands at the fringe of conurbations have more chance of being converted into built-up areas due to the favourable topography and the accessibility to existing infrastructure, being in the vicinity of urban areas. We analysed the global land-take during the period 2000-2014. The data are based on a global dataset describing the spatial evolution of human settlements using the Global Human Settlement Layer, which was derived from Landsat images collected in 1975, 1990, 2000 and 2014. Although the global land-take represents roughly 0.1% of the global terrestrial Earth, it affects 1% of the naturally fertile soils, according to the proposed Soil Productivity Indexes (SPI), based upon the potential soil productivity, calculated on the basis of the Harmonized World Soil Database. We have found that, few large conurbations develop on potentially high productive soil, while scarcely productive soils sustain the expansion of several megalopolises. On a global scale and through the centuries, considered comparatively as individual overall age of settlements, a trend between the intrinsic quality of the soils and its use for settlement purposes as major competitor, was not observed.

Strawberry fields forever: That is, how many grams of plastics are used to grow a strawberry?

In important areas of the agricultural sector, plastic is one of the most important tools for improving the managerial and economic performance of companies. Horticultural production under protected cultivation, in particular, makes abundant use of plastic polymers-polymers that mostly turn into waste. This study aims to identify similar organisational models among farmers operating in the strawberry production sector in relation to their intention to recycle plastic material and which factors characterize these models (attitudes, subjective norms, perceived behavioural control, and socio-demographic characteristics) and to quantify the plastic used in relation to the different types of materials used. In order to achieve this aim, data coming from one of Italy's most specialized areas in the strawberry sector, Marsala, were analysed using a principal component analysis to identify latent factor and subsequently clustered in order to identify similar organisational models. The main findings show that behind a typical 250-gram basket of strawberries, the production process has generated more than 20 g of plastic waste, consisting of different polymers. In addition, younger farmers, who manage companies of smaller physical dimensions, and with a higher level of education, show a greater intention to join plastic recycling programs, guided by their attitudes, by the necessity to respond to social pressures, and by the ability to manage recycling programs. Our main result adds to previous knowledge that the informed producer (via greater awareness) is the key to solving the problem through improving environmental sustainability and creating a circular economy.

Resource or waste? A perspective of plastics degradation in soil with a focus on end-of-life options.

'Capable-of-being-shaped' synthetic compounds are prevailing today over horn, bone, leather, wood, stone, metal, glass, or ceramic in products that were previously left to natural materials. Plastic is, in fact, economical, simple, adaptable, and waterproof. Also, it is durable and resilient to natural degradation (although microbial species capable of degrading plastics do exist). In becoming a waste, plastic accumulation adversely affects ecosystems. The majority of plastic debris pollutes waters, accumulating in oceans. And, the behaviour and the quantity of plastic, which has become waste, are rather well documented in the water, in fact. This review collects existing information on plastics in the soil, paying particular attention to both their degradation and possible re-uses. The use of plastics in agriculture is also considered. The discussion is organised according to their resin type and the identification codes used in recycling programs. In addition, options for post-consumer plastics are considered. Acknowledged indicators do not exist, and future study they will have to identify viable and shared methods to measure the presence and the degradation of individual polymers in soils.

Multitemporal mapping of peri-urban carbon stocks and soil sealing from satellite data.

Peri-urbanisation is the expansion of compact urban areas towards low-density settlements. This phenomenon directly challenges the agricultural landscape multifunctionality, including its carbon (C) storage capacity. Using satellite data, we mapped peri-urban C stocks in soil and built-up surfaces over three areas from 1993 to 2014 in the Emilia-Romagna region, Italy: a thinly populated area around Piacenza, an intermediate-density area covering the Reggio Emilia-Modena conurbation and a densely anthropized area developing along the coast of Rimini. Satellite-derived maps enabled the quantitative analysis of spatial and temporal features of urban growth and soil sealing, expressed as the ratio between C in built-up land and organic C in soils (Cc/Co). The three areas show substantial differences in C stock balance and soil sealing evolution. In Piacenza (Cc/Co=0.07 in 1993), although questioned by late industrial expansion and connected residential sprawl (Cc/Co growth by 38%), most of the new urbanisation spared the best rural soils. The Reggio Emilia-Modena conurbation, driven by the polycentricism of the area and the heterogeneity of economic sectors (Cc/Co rising from 0.08 to 0.14 from 1993 to 2014), balances sprawl and densification. Rimini, severely sealed since the 1960s (Cc/Co=0.23 in 1993), densifies its existing settlements and develops an industrial expansion of the hinterland, with Cc/Co growth accelerating from +15% before 2003 to +36% for the last decade.

Release of phosphorus under reducing and simulated open drainage conditions from overfertilised soils.

Does removal of cations from soil solution during soil reduction stimulate phosphorus (P) release? An ion-exchange resin system was employed to provide a sink during the incubation of twelve soils under fully reducing conditions. This experimental design was considered to better simulate the loss of ions likely to occur under field conditions than more routine batch type closed extraction systems where solutes build-up in the extract solution. The small solute concentrations that remain in the equilibrating solution suggest the mixed resin system acted as an effective sink over the whole experimental period. By maintaining a small P concentration the resin system mimics soil drainage conditions and encourages P release from soil. Measurement of soil P forms by sequential extraction after the incubation period indicated an increase in the amorphous forms present. Here we show that even if the P-containing solution is retained by the soil, the potential for a subsequent P loss is increased under aerobic conditions. The management of drainage systems should try and avoid the onset of anaerobic conditions. Eventually, magnesium- and calcium-based precipitation products could recapture P from drains recycling it in topsoils as fertilizer.

DRIFTS sensor: soil carbon validation at large scale (Pantelleria, Italy).

A fast and accurate measurement of soil carbon is needed in current scientific issues. Today there are many sensors suitable for these purposes, but choosing the appropriate sensor depends on the spatial scale at which the studies are conducted. There are few detailed studies that validate these types of measures allowing their immediate use. Here it is validated the quick use of a sensor in execution at Pantelleria, chosen for size, use and variability of the parameter measured, to give an operational tool for carbon stocks studies. The DRIFT sensor used here has been validated in the first 60 cm of the soil of the whole island, and it has shown predictivity higher than 90%.

The scent of Mare Nostrum: medicinal and aromatic plants in Mediterranean soils.

In the Mediterranean area, the simultaneous occurrence of pedological, climatic and economic constraints often sets a limit on the profitability of agriculture, and farmers are forced to grow a reduced number of species, dealing with a secure-albeit low-market income. The introduction of medicinal and aromatic plants (MAPs) inside the current farming systems could represent a useful means to meet the multifunctional role of agriculture: producing safe food, in respect of the environment, and contributing to the development of rural areas. The study of the relationships between MAPs and the soils in which they may be grown may have two approaches: (1) the evaluation of yield and qualitative response of MAPs to the variation of soil features; and (2) the study of selective recovery of certain elements (toxic and beneficial), and their subsequent release in herbal products. In many MAPs, significant variations of plant characteristics have been ascertained with varying soil traits, and the selective recovery and subsequent release in food of certain elements have been demonstrated. Hence, great attention must be paid to the choice of soil and cropping strategies, to obtain satisfactory yields of high quality and best-priced products, respecting their safety and nutritional value.

Are agricultural soils under a continental temperate climate susceptible to episodic reducing conditions and increased leaching of phosphorus?

Soil science research has probably underestimated the significance that short-term, episodic cycles of reduction and oxidation has had on phosphorus (P) reactivity. Here, the effects of eleven pulsed reduction-oxidation (including wet-dry) cycles on soil P dynamics are compared for 12 soils having contrasting properties and all overfertilised with respect to P. The laboratory based incubation conditions attempted to simulate transient waterlogging of the soil profile and involved repeated sampling and analysis of both the solution and solid phase P forms. An initial increase in P concentration in solution that occurred up to and including the fourth full cycle was followed by a sharp decline in concentration for all but one soil. Accompanying changes in the main extractable forms of P, which appeared to be cumulative, could be summarised as a general decline in the organic P fraction and an overall increase in amorphous associated inorganic forms of P. The fact that up to 60% of the total soil P was demonstrated to change its sensitivity for a particular extractant suggests that these operationally defined P forms are susceptible to transformation as a consequence of changing environmental conditions. There was also a suggestion that certain of the changes in P forms were irreversible. While the laboratory conditions imposed do represent extreme conditions the soils only experienced cyclic changes in their moisture regime. If timing and frequency of intense precipitation events are likely to increase, as predicted in many climate change scenarios, then these results suggest that the effects of episodic redox pulses may have implications for P cycling in agricultural soils.

Influence of 150 years of land use on anthropogenic and natural carbon stocks in Emilia-Romagna region (Italy).

Changes in land use/cover that are commonly associated with urbanization can dramatically influence the amount, chemical form, and spatial distribution of carbon (C) stocks. Measured values and relative literature for composition of natural and anthropogenic materials have been compiled. These data are used in conjunction with land cover statistics and expert assessment of building design to calculate C stocks associated with 150 years of land use change and development for an area of the Po River Valley, Northern Italy. Using 4 time periods (1853, 1954, 1976, and 2003), we demonstrate that the C stocks within this densely populated area have undergone considerable modification. A 52% increase in population density has been associated with an increase in the proportion of total C stored in anthropogenic stocks from 0.2% to 6%; this has been accompanied by a one order of magnitude increase in the carbon emission per capita per unit area. These changes have also been accompanied by a major shift in stocks from organic to inorganic forms of C.

Some effects of a buried electricity transmission cable on bulk soil.

A case study in NW Italy investigating an underground electric line (1 m depth triple cable at operative voltages 220-380 kV) measured electric fields in the surrounding soil virtually close to zero but magnetic fields (microTs) 20 times the background level. After 6 months, the influence radius around the cable on microbial activity (estimated by soil ATP), organic carbon, and total nitrogen follows exactly the inverse trend of the MF, shifting the biological activity with a lag distance of 5 m from the 220 kV cable.