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2.
Mem Inst Oswaldo Cruz ; 97(6): 897-900, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12386718

ABSTRACT

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.


Subject(s)
Chlamydia Infections/diagnosis , Chlamydia trachomatis/isolation & purification , Polymerase Chain Reaction/methods , Base Sequence , DNA Primers , False Negative Reactions , Humans , Sensitivity and Specificity
3.
Mem. Inst. Oswaldo Cruz ; 97(6): 897-900, Sept. 2002. ilus
Article in English | LILACS | ID: lil-320158

ABSTRACT

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis


Subject(s)
Humans , Chlamydia Infections , Chlamydia trachomatis , Polymerase Chain Reaction , Base Sequence , DNA Primers , False Negative Reactions , Sensitivity and Specificity
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