Widespread survey finds no evidence of Haplosporidium nelsoni (MSX) in Gulf of Mexico oysters.

The advent of molecular detection assays has provided a set of very sensitive tools for the detection of pathogens in marine organisms, but it has also raised problems of how to interpret positive signals that are not accompanied by visual confirmation. PCR-positive results have recently been reported for Haplosporidium nelsoni (MSX), a pathogen of the oyster Crassostrea virginica in 31 of 40 oysters from 6 sites in the Gulf of Mexico and the Caribbean Sea. Histological confirmation of the PCR results was not undertaken, and no haplosporidian has been reported from the numerous histological studies and surveys of oysters in the region. To further investigate the possibility that H. nelsoni is present in this region, we sampled 210 oysters from 40 sites around the Gulf of Mexico and Puerto Rico using PCR and 180 of these using tissue-section histology also. None of the oysters showed evidence of H. nelsoni by PCR or of any haplosporidian by histology. We cannot, therefore, confirm that H. nelsoni is present and widespread in the Gulf of Mexico and the Caribbean Sea. Our results do not prove that H. nelsoni is absent from the region, but taken together with results from previous histological surveys, they suggest that for the purposes of controlling oyster importation, the region should continue to be considered free of the parasite.

Minchinia mercenariae n. sp. (Haplosporidia) in the hard clam Mercenaria mercenaria: implications of a rare parasite in a commercially important host.

During routine histopathology of 180 juvenile hard clams, Mercenaria mercenaria, from a site in Virginia, USA, in 2007, we discovered a single individual heavily infected with a parasite resembling a haplosporidian, some members of which cause lethal bivalve diseases. Scanning electron microscopy of spores and sequencing of small subunit ribosomal DNA confirmed a new species: Minchinia mercenariae n. sp. Further sampling of clams at the site found prevalences up to 38% using polymerase chain reaction (PCR). No parasites were found in routine histological screening of the same individuals, but re-examination of clams judged positive by in situ hybridization (ISH) revealed very faintly staining plasmodia. No unusual mortalities have occurred among the sampled groups. Analysis of clams from Massachusetts to Florida by PCR failed to detect the parasite, but a haplosporidian found in a clam from New Jersey in 2001 was subsequently identified by ISH as M. mercenariae. No other haplosporidians have been reported in thousands of hard clams from the US east coast examined histologically since the mid-1980s. The discovery underscores critical questions about how to assess the risks associated with parasites in groups known to be lethal, but that themselves are not considered a problem.

Perkinsus chesapeaki in stout razor clams Tagelus plebeius from Delaware Bay.

Perkinsus chesapeaki is reported from stout razor clams Tagelus plebeius in Delaware Bay, extending the known range of P. chesapeaki north of Chesapeake Bay. P. marinus, which causes dermo disease, is prevalent in cultured and wild oysters at this site, but was not detected in T. plebeius. Evidence for the presence of disseminated neoplasia, also reported from Chesapeake Bay, was equivocal. Although P. chesapeaki infections were associated with mortality events, light infection intensities and a general lack of histopathological evidence of disease limit inferences about a causal relationship. A comparison of Ray's fluid thioglycollate medium (RFTM)-based and PCR-based detection assays highlight differences in detection capabilities related to the quantity and type of tissue processed rather than assay sensitivity per se, a point that should be considered when surveying populations for disease prevalence. Investigators are further cautioned to use care when applying and interpreting diagnostic assays when used with novel species.