ABSTRACT
Efficient routes to alpha-tert-butyl- and alpha-iso-propyl-ortho-hydroxybenzylamines 1a and 1b are described. Highly enantioenriched 1a and 1b were obtained by resolution of the methoxy derivatives 2 by recrystallization of the salts formed with mandelic acid followed by Lewis acid mediated demethylation. The chiral 1,3-amino alcohol 1a has also been obtained in an asymmetric synthesis with the key step a diastereoselective alkylation of the imine obtained by condensation of o-anisaldehyde with phenyl glycinol. The absolute stereochemistry of these 1,3-aminophenols was determined by CD spectroscopy of the salicylideneamines 12 and by an X-ray structure analysis of the salt formed between (R)-mandelic acid and (S)-alpha-tert-butyl-ortho-methoxybenzylamine ((S)-2a).
ABSTRACT
We studied the survival of human cytomegalovirus inoculated experimentally into saliva and urine-soaked paper diapers. Infectious virus could be recovered from saliva maintained at room temperature or 37 degrees C for up to 2 hours after inoculation. Cytomegalovirus survived in paper diapers for periods as long as 48 hours, with the quantity of virus in urine remaining relatively constant for the initial 12 hours. These observations provide further support for the concept that fomites may have a role in the transmission of cytomegalovirus infections.
