Combination Insecticide Treatments with Methoprene and Pyrethrin for Control of Khapra Beetle Larvae on Different Commodities.

Trogoderma granarium Everts, the khapra beetle, is a serious pest of stored products throughout the world. Larvae pose a significant threat to stored products because they feed on >100 different commodities, possess the ability to enter facultative diapause, and are difficult to detect. Control methods for T. granarium include fumigation, contact insecticides, trapping, and insecticide-incorporated packaging. The objective of this study was to determine the residual efficacy of two insecticide formulations (methoprene + deltamethrin + piperonyl butoxide synergist Gravista® and methoprene + deltamethrin, DiaconIGR®Plus). These insecticides were evaluated on three stored product commodities, corn, wheat, and brown rice, by exposing T. granarium larvae during a 12-month testing period. Both formulations significantly reduced adult emergence on corn and wheat for 12 months and on brown rice for up to 6 months. Adult emergence was highest at month 12 for corn (8.41%), and brown rice (85.88%), and month 9 for wheat (39.52%), treated with DiaconIGR®Plus or Gravista®, respectively. A biological index used to measure the development of exposed larvae on the treated grain from the larval stage (low values) to adult emergence (high values) was lower (fewer adults) on corn and wheat compared to controls. Despite differences in formulations, each of these grain protectants could be utilized by stored commodity managers to protect commodities during storage and transportation for T. granarium when and if this pest is detected at USA ports of entry.

Aerosolized Insecticide Spray Distributions and Relationships to Efficacy against Stored Product Pests.

Aerosol insecticides are widely used in stored product insect management programs in food facilities. Previous research has shown spatial variation in aerosol efficacy within facilities, but information on how spatial patterns of aerosol droplet concentration, size distribution, dispersal, and deposition contribute to this variation in efficacy is limited. This study involved two aerosol application systems: a high-pressure cylinder containing TurboCide Py-75® with pyriproxyfen IGR (ChemTech Ltd., Des Moines, IA, USA) and a hand-held fogger containing Pyrocide 100® (MGK, Minneapolis, MN, USA) with Diacon II which contains methoprene IGR (Wellmark, Schaumburg, IL, USA). These systems were used at single or multiple application locations. The spray trials were conducted in a small-scale flour mill, Hall Ross Flour Mill (Kansas State University, Manhattan, KS, USA). The droplet size distributions were monitored at multiple positions within the room using nine aerodynamic particle sizing (APS, TSI Incorp, Shoreview, MN, USA) instruments. The APS data collected over the treatment period were summarized into a mass concentration index (MCI), which ranged from 155 to 2549 mg/m3 for Turbocide and 235-5658 mg/m3 for Pyrocide. A second parameter called the Deposition Index (Dep.Idx) was derived to estimate potential insecticide depositions on the floor and has units of g/m2. The Dep.Idx was below 5.3 g/m2 for most Turbocide applications, while the Dep.Idx was below 8.4 g/m2 for most Pyrocide applications. The MCI and Dep.Idx values varied with APS position and spray application location, with proximity to the aerosol application location and degree of obstruction between the release point and APS position contributing to this variation. We assessed the relationship between aerosol droplet parameters and insect efficacy using Tribolium confusum Jacqueline DuVal, the confused flour beetle. The adults were treated directly, while the larvae were treated two weeks later during the residual test (previously published). For Turbocide, efficacy against adults increased with MCI and Dep.Idx values, but for residual efficacy of the IGR, efficacy was high at all aerosol droplet values, so no relationship was apparent. In contrast, the relationship between Pyrocide deposition and adult insect efficacy was highly variable. But with larval insect efficacy, residual larvae control was directly related to increases in Pyrocide MCI and Dep.Idx. Contour plots of Dep.Idx values were developed, which could be used to predict areas of the mill that are not receiving an adequate application rate, and this could be used to develop more effective application strategies for aerosol insecticides in food facilities.

Comparison of different traps and attractants in 3 food processing facilities in Greece on the capture of stored product insects.

Certain lures are marketed toward particular pests or classes of pests, while others might be multi-species lures. Investigative aims for this study included both which trap was most sensitive and whether different combinations of traps and attractants were delivering novel information about the stored product insect community. Comparisons were made for all combinations of 3 commercial traps and 4 different attractants plus an untreated control on the capture of stored-product insects for 2 consecutive years in 3 food processing facilities in Central Greece. The traps used in the experiments were Dome Trap (Trécé Inc., USA), Wall Trap (Trécé) and Box Trap (Insects Limited, Ltd., USA). The attractants that were evaluated were 0.13 g of (i) PantryPatrol gel (Insects Limited), (ii) Storgard kairomone food attractant oil (Trécé), (iii) wheat germ (Honeyville, USA), and (iv) Dermestid tablet attractant (Insects Limited). The traps were inspected approximately every 15 days and rotated. A total of 34,000+ individuals were captured belonging to 26 families and at least 48 species. The results indicated that Indian meal moth, Plodia interpunctella (Hübner), red flour beetle, Tribolium castaneum (Herbst), and cigarette beetle, Lasioderma serricorne (F.) were the most abundant. Although there were noticeable differences among the different traps and attractants for specific species, all combinations provided similar information on population dynamics. Generally, Dome traps baited with either the oil or the gel, were found to be the most sensitive. The results of the present study demonstrate the importance of long-term trapping protocols, as a keystone in IPM-based control strategies in food processing facilities.

Long-Lasting Insecticide-Treated Netting Affects Reproductive Output and Mating Behavior in Tribolium castaneum (Coleoptera: Tenebrionidae) and Trogoderma variabile (Coleoptera: Dermestidae).

Preventing insect infestations is a critical component for establishing a pest management plan for stored-product insects. Long-lasting insecticide-treated netting (LLIN) is a potential tool to reduce insect movement by providing a chemical barrier, where insects may be able to physically pass through but ultimately die after exposure to the netting. Sublethal effects, such as reduced movement immediately after exposure and reduced ability to colonize, have been reported. Here we examine the sublethal effects of exposure to LLIN on two beetle species, Trogoderma variabile Ballion, warehouse beetle, and Tribolium castaneum Herbst, red flour beetle. We found that both female and male T. castaneum exposed to LLIN produced significantly less adult progeny than those exposed to untreated netting. Adult progeny output did not differ for T. variabile, but survivorship increased in T. variabile females exposed to LLIN. Importantly, the overall net reproductive rate was significantly decreased for both T. variabile and T. castaneum. The number of copulation attempts did not differ between males or females exposed to LLIN compared to untreated netting, but males exposed to LLIN showed increased durations of attempted and successful copulation events. This research demonstrates that the implications of LLIN exposure extend past direct mortality, with sublethal effects on reproductive output potentially increasing the effectiveness of this tool for preventing insect infestations.

Sublethal exposure of Trogoderma granarium everts (Coleoptera: Dermestidae) to insecticide-treated netting alters thigmotactic arrestment and olfactory-mediated anemotaxis.

Long-lasting insecticide treated netting (LLIN) has a number of potential uses for the control of insect pests. Using such netting, stored products may be protected from insects including the khapra beetle (Trogoderma granarium Everts, Coleoptera: Dermestidae) a widespread pest of many agricultural commodities. Here we first examined whether brief exposures of larvae to LLIN, for less than 30 min, decreased the chance of eventual adult emergence compared to larvae exposed on untreated netting. Next, we observed the responses of larvae that were either not exposed to any netting, exposed to untreated netting, or exposed to LLIN for 10 min and then placed in a wind tunnel and monitored for movement toward a stimulus. The wind-tunnel assay was performed either with or without a lure containing kairomones and pheromones known to be attractive to larvae of this species. There was little effect of the LLIN on adult emergence of exposed larvae. However, there were interacting effects of untreated netting and LLIN relating to thigmotaxis and anemotaxis. Larvae not exposed to netting showed increased likelihood of walking upwind if the semiochemical lure was provided, as expected. A similar pattern was observed when the untreated netting was used, but the larvae became more likely to remain stationary in the assay after acclimating to the net. When LLIN was used, the larvae became more likely to move and there was a baseline increase in the likelihood of moving upwind. However, upwind walking was no longer related to semiochemical presentation. These observations suggest that particular care should be used in relation to the airflow patterns and semiochemical landscape of the warehouse settings in which LLIN is deployed.

Effects of Aerosol Insecticide Application Location on the Patterns of Residual Efficacy Against Tribolium confusum (Coleoptera: Tenebrionidae) Larvae.

Aerosol insecticides are one tool that pest management professionals can utilize as a spatial treatment inside food facilities and storage warehouses. Methods of aerosol application can vary significantly and can affect the spatial pattern of efficacy achieved. We investigated how the location from which an aerosol insecticide is applied inside a mill influenced the spatial dispersal of the insecticide. Treatments were performed using two commercial formulations, pyrethrin + pyriproxyfen (insect growth regulator [IGR]) and pyrethrin + methoprene (IGR), applied at one of three static locations or a fourth application comprising of splitting the application among all three locations. Concrete arenas were placed out at different locations within the mill during applications. At 2, 4, and 6 wk post-aerosol application, Tribolium confusum Jacquelin du Val, confused flour beetle, larvae were added to the concrete arenas and monitored for development and efficacy was evaluated based on percent adult emergence and an efficacy index that ranged from 1 (low) to 21 (high). The spatial pattern of aerosol coverage varied between insecticide formulations and the aerosol application location. Areas of the mill near walls, corners, equipment, and farthest away from the application location had larger zones of low efficacy index values among all four application locations. This study illustrated that the aerosol insecticide formulation, application location, and delivery method all significantly influenced residual efficacies of the insecticides. To increase the overall spatial coverage and IGR efficacy, targeting these areas of a mill floor with the aerosol or additional intervention techniques would increase uniform coverages and overall effectiveness.

Bioassays and Methodologies for Insecticide Tests with Larvae of Trogoderma granarium (Everts), the Khapra Beetle.

New insecticide treatment options would be beneficial for control programs for Trogoderma granarium Everts, the khapra beetle, in the United States. Two insecticides were evaluated, the Polyzone® formulation of deltamethrin and a formulation of the insect growth regulator methoprene combined with deltamethrin and the synergist piperonyl butoxide. In the test with Polyzone® deltamethrin, concrete arenas were treated with a low and high rate, and held outside, inside a shed, or inside a lab. Compared to storage in the lab, residue degradation increased slightly in the shed, and then further outside, as evidenced by greater larval survival and adult emergence. Across all environmental treatments, the high rate was more effective than the lower rate. For the combination methoprene product, the effect of food contact with treated surfaces was examined. When treating arenas with food and transferring the food to clean dishes, there was no immediate effect on larval survival, but there was a reduction in survival and emergence to the adult stage after one month. For both tests, larvae apparently often went into diapause after they were introduced onto the treatment arenas. Both treatments could be utilized in management programs if T. granarium infestations are detected.

Case Study: A Practical Application of an Aerosol Treatment in a Commercial Mill.

In recent years, there has been an increasing interest and need for alternatives to structural fumigations, and one alternative that has been used across the industry is aerosol insecticides. Previous tests inside a pilot-scale mill demonstrated that aerosol particle size, delivery method, and the spatial configuration of the mill all influenced effectiveness. However, there is no research conducted inside large commercial facilities. The objective of this research was to evaluate a pyrethrin-plus-methoprene aerosol application inside a commercial mill on adult Tribolium confusum Jacquelin duVal, confused flour beetle, directly exposed to the aerosol and residual effects on larvae. Additionally, five aerodynamic particle sizer spectrometers were placed in the facility and recorded instantaneous spray concentration and estimated aerosol deposition. Adult T. confusum exposed nearest to the aerosol application points had the highest percentage of affected adults (>60%). The aerosol also had vertical movement when released at the top of a three-story open room; instantaneous concentrations were recorded on the ground floor. The aerosol residual was highly effective after 6-weeks post aerosol exposure, as 80% of the bioassays did not have any adult emergence from exposed larvae. This research demonstrates a practical use of aerosol insecticides and their potential to be an effective alternative to structural fumigations.

Mobility of Adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) After Exposure to Long-Lasting Insecticide-Incorporated Netting.

Stored products represent an enormous economic output, but insects regularly immigrate into stored products from the surrounding landscape throughout the postharvest supply chain. Long-lasting insecticide-incorporatednetting (LLIN), which usually contains an incorporated pyrethroid, has been used as part of a strategy to reduce the spread of malaria in tropical regions since the 1990s and has only recently been considered for its application in pre- and postharvest agricultural contexts. The goal of this study was to determine how short-term exposure to LLINs in the laboratory impacts the locomotory behavior and mortality for adult Tribolium castaneum (Herbst), (Coleoptera: Tenebrionidae) red flour beetle, and Rhyzopertha dominica (Fauvel), (Coleoptera: Bostrichidae) lesser grain borer, at different periods of time after exposure. Exposure to LLINs resulted in multiple-fold reductions in the distance moved and elevated angular velocity in both species that quickly took effect and persisted even after 168 h compared with adults exposed to control netting. R. dominica was somewhat more susceptible than T. castaneum to LLINs. Finally, the dispersal capacity of both species, measured as ability to move to a remote resource patch, was significantly impaired or absent after exposure to LLINs compared to adults exposed to control netting. Our results demonstrate that LLINs are a promising new technology for reducing infestation by stored product insects since even short exposures limit movement and ultimately lead to knockdown and death.

Aerosol Dispersal Patterns and Resulting Effects on Tribolium confusum (Coleoptera: Tenebrionidae) Adults.

Aerosol application of insecticides as part of an integrated pest management program is becoming more widely adopted in food facilities such as flour mills. However, the method of application and the complex structural features within a facility can impact how insecticide particles travel and settle on surfaces and therefore impact the consistency in efficacy obtained. Here we investigated how the location from which an aerosol insecticide is released impacts the spatial pattern of aerosol deposition, using efficacy against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), the confused flour beetle, as a model insects. Concrete bioassay arenas containing T. confusum adults and flour were exposed to two commercial aerosol formulations, pyrethrin + pyriproxyfen and pyrethrin + methoprene, and observed for initial adult knockdown and subsequent mortality. Aerosols were applied from one of three static locations or a fourth application comprised of multiple aerosol release points. The aerosol release position had a significant effect on T. confusum adult knockdown and mortality; both ranged from 0 to 100% depending on bioassay arena location. The multiple aerosol release positions tended to have more bioassay arenas with higher knockdown and mortality, and had a more consistent impact across all arena locations, yet there were still areas on the mill floor where little adult beetle efficacy was observed. The effect of each aerosol on T. confusum adults is a culmination of how the aerosol was applied, the distance the aerosol particles traveled, and the complexity of a milling facility.

Evaluating Penetration Ability of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) Larvae into Multilayer Polypropylene Packages.

The larvae of the Indian meal moth, Plodia interpunctella (Hübner), can invade or penetrate packaging materials and infest food products. Energy bars with three polypropylene packaging types were challenged with eggs (first instars), third instars, and fifth instars of P. interpunctella to determine package resistance at 28 °C and 65% r.h. The packing types were also challenged with two male and two female pupae of P. interpunctella under similar conditions in order to determine which package provided the greatest protection against larval penetration. Samples infested with eggs, third instars, and pupae were evaluated after 21 days and 42 days to count the number of larvae, pupae, and adults found inside the packages. Packages challenged with fifth instars were observed after 21 days to count the number of larvae, pupae, and adults inside each package. The number and diameter of the holes were determined in each package, followed by the amount of damage sustained to the energy bar. Third and fifth instars showed a higher tendency to penetrate all of the packaging types. First instars showed a reduction in package penetration ability compared with third and fifth instars. The increase in exposure time resulted in an increase in the damage sustained to the energy bars. Among packaging types, the thickest package (Test A) was most resilient to penetration by all of the larval stages. In conclusion, energy bar manufacturers need to invest more effort into improving packaging designs, creating thicker gauge films, or advancing odor barrier technology, in order to prevent penetration and infestation by P. interpunctella larvae.