ABSTRACT
Omega (n-)3 and n-6 long chain polyunsaturated fatty acids (LCPUFA) accumulation in the infant brain after birth is strongly driven by dietary supply of n-3 and n-6 LCPUFAs and their C18 precursors through breast milk or infant formula. n-3 LCPUFA accretion is associated with positive effects on neurodevelopmental outcome whereas high n-6 LCPUFA accumulation is considered disadvantageous. Maternal diet is crucial for breast milk fatty acid composition. Unfortunately, global increases in linoleic acid (C18:2n-6; LA) intake have dramatically increased n-6 LCPUFA and reduced n-3 LCPUFA availability for breastfed infants. We investigated the effects of reducing maternal dietary LA, or increasing n-3 LCPUFA, during lactation on milk and offspring brain fatty acids in mice. Offspring brain n-3 LCPUFA was higher following both interventions, although effects were mediated by different mechanisms. Because of competitive interactions between n-3 and n-6 fatty acids, lowering maternal LA intake may support neurodevelopment in breastfed infants.
Subject(s)
Brain Chemistry , Fatty Acids, Omega-3/administration & dosage , Fatty Acids, Unsaturated/analysis , Lactation/metabolism , Animals , Animals, Newborn , Animals, Suckling/blood , Brain/growth & development , Brain/metabolism , Dietary Supplements , Female , Linoleic Acid/adverse effects , Male , MiceABSTRACT
Epidemiological studies have shown an association between short or disrupted sleep and an increased risk to develop obesity. In animal studies, however, sleep restriction leads to an attenuation of weight gain that cannot be explained by changes in energy intake. In the present study, we assessed whether the attenuated weight gain under conditions of restricted sleep is a consequence of an overall increase in energy expenditure. Adult male rats were subjected to a schedule of chronic sleep restriction (SR) for 8 days with a 4h window of unrestricted rest per day. Electroencephalogram and electromyogram recordings were performed to quantify the effect of the sleep restriction schedule on sleep-wake patterns. In a separate experiment, we measured sleep restriction-induced changes in body weight, food intake, and regulatory hormones such as glucose, insulin, leptin and corticosterone. To investigate whether a change in energy expenditure underlies the attenuation of weight gain, energy expenditure was measured by the doubly labeled water method from day 5 until day 8 of the SR protocol. Results show a clear attenuation of weight gain during sleep restriction but no change in food intake. Baseline plasma glucose, insulin and leptin levels are decreased after sleep restriction which presumably reflects the nutritional status of the rats. The daily energy expenditure during SR was significantly increased compared to control rats. Together, we conclude that the attenuation of body weight gain in sleep restricted rats is explained by an overall increase in energy expenditure together with an unaltered energy intake.
Subject(s)
Body Weight/physiology , Energy Metabolism/physiology , Sleep Deprivation/metabolism , Sleep Deprivation/physiopathology , Analysis of Variance , Animals , Blood Glucose , Corticosterone/blood , Eating , Electroencephalography , Insulin/blood , Male , Radioimmunoassay , Rats , Rats, Wistar , Sleep Stages/physiology , Time Factors , WakefulnessABSTRACT
Olanzapine is an a-typical antipsychotic drug antagonizing predominantly 5-HT and dopamine, but also histamine, muscarin, and α-adrenergic receptors. In humans, Olanzapine induces weight gain and increases the risk of type 2 diabetes. The underlying mechanisms of Olanzapine-induced weight gain are unclear. To study this we administered Olanzapine (5mg/kg) in female Wistar rats on a medium fat diet for 14 days via a permanent gastric catheter twice a day, just prior to the onset and at the middle of dark phase. Food and water intake, locomotor activity and body temperature were measured. Olanzapine acutely induced hypothermia, markedly decreased locomotor activity and increased body weight during 14 days of treatment. Olanzapine treatment did not result in an alteration of 24h food intake, but diurnal patterns of feeding behavior and body temperature were dramatically changed. We conclude that in female Wistar rats Olanzapine has an acute hypothermic effect, that the effect of Olanzapine on feeding behavior is secondary to the effect on activity, and that Olanzapine-induced weight gain is primarily the result of reduction in locomotor activity.
Subject(s)
Benzodiazepines/toxicity , Feeding Behavior/drug effects , Feeding Behavior/psychology , Hypothermia/chemically induced , Motor Activity/drug effects , Weight Gain/drug effects , Animals , Drug Administration Schedule , Feeding Behavior/physiology , Female , Hypothermia/physiopathology , Motor Activity/physiology , Olanzapine , Rats , Rats, Wistar , Sucrose/administration & dosage , Weight Gain/physiologyABSTRACT
Voluntary physical activity may be related to personality traits. Here, we investigated these relations in two mouse lines selectively bred for high voluntary wheel-running behavior and in one non-selected control line. Selection lines were more explorative and "information gathering" in the open-field test, either with increased upright positions or horizontal locomotion toward the middle ring. Furthermore, one of the selection lines had an increased risk-taking behavior relative to the control line in approaching a novel object placed in the center of the open field. However, anxiety behavior was increased in selection lines during the plus-maze test. Maze learning was not statistically different among lines, but routine behavior was increased in both selection lines when the maze exit after 2 days of testing was displaced. Specifically, in the displaced maze, selected mice traveled more frequently to the old, habituated exit, bypassing the new exit attached to their home cage. Although the generality of the results would need to be confirmed in future studies including all eight lines in the selection experiment, the increased routine and exploratory behavior (at least in the lines used in the present study) may be adaptive to sustain high activity levels.
Subject(s)
Biological Evolution , Exploratory Behavior/physiology , Maze Learning/physiology , Motor Activity/physiology , Selection, Genetic , Animals , Animals, Outbred Strains , Anxiety , Breeding/methods , Female , Male , Mice/genetics , Models, Animal , Risk-TakingABSTRACT
Fluctuations in substrate preference and utilization across the circadian cycle may be influenced by the degree of physical activity and nutritional status. In the present study, we assessed these relationships in control mice and in mice from a line selectively bred for high voluntary wheel-running behavior, either when feeding a carbohydrate-rich/low-fat (LF) or a high-fat (HF) diet. Housed without wheels, selected mice, and in particular the females, exhibited higher cage activity than their non-selected controls during the dark phase and at the onset of the light phase, irrespective of diet. This was associated with increases in energy expenditure in both sexes of the selection line. In selected males, carbohydrate oxidation appeared to be increased compared to controls. In contrast, selected females had profound increases in fat oxidation above the levels in control females to cover the increased energy expenditure during the dark phase. This is remarkable in light of the finding that the selected mice, and in particular the females showed higher preference for the LF diet relative to controls. It is likely that hormonal and/or metabolic signals increase carbohydrate preference in the selected females, which may serve optimal maintenance of cellular metabolism in the presence of augmented fat oxidation.
Subject(s)
Breeding , Circadian Rhythm/genetics , Fats/metabolism , Feeding Behavior/physiology , Oxidation-Reduction , Running/physiology , Animals , Calorimetry, Indirect/methods , Eating/genetics , Female , Food Preferences/physiology , Male , Mice , Motor Activity/genetics , Oxygen Consumption/genetics , Respiration/genetics , Sex FactorsABSTRACT
The aim of the study was develop to an animal model that links coping style to insulin resistance. We hypothesized that the psychogenetically selected Roman Low Avoidance (RLA) rats may serve as such a model. To test this hypothesis, we submitted both RLA and Roman High avoidance (RHA) rats to a series of intravenous glucose tolerance tests (IVGTT). These IVGTT were followed by post mortem metabolic characterization of the selection lines. It was found that plasma insulin levels are markedly elevated in the passively coping RLA rat, both in baseline conditions and during the intravenous glucose tolerance tests. The elevation in plasma insulin was accompanied with increased levels of plasma corticosterone, FFA, leptin and triglycerides but not by changes in body weight. We conclude that the passive, highly emotional RLA rat is metabolically different from both the RHA rat and the standard control Wistar rat and may serve as a non-obese animal model for insulin resistance.
Subject(s)
Avoidance Learning/physiology , Disease Models, Animal , Insulin Resistance/physiology , Rats, Inbred Strains/genetics , Analysis of Variance , Animals , Behavior, Animal , Blood Glucose/physiology , Body Weight/physiology , Drinking/physiology , Eating/physiology , Exploratory Behavior , Glucose Tolerance Test , Insulin/blood , Leptin/blood , Male , RatsABSTRACT
In parallel with increased prevalence of overweight people in affluent societies are individuals trying to lose weight, often using low-carbohydrate diets. Nevertheless, long-term metabolic consequences of those diets, usually high in (saturated) fat, remain unclear. Therefore, we investigated long-term effects of high-fat diets with different carbohydrate/protein ratios on energy balance and fuel homeostasis in obese (fa/fa) Zucker and lean Wistar rats. Animals were fed high-carbohydrate (HC), high-fat (HsF), or low-carbohydrate, high-fat, high-protein (LC-HsF-HP) diets for 60 days. Both lines fed the LC-HsF-HP diet displayed reduced energy intake compared with those fed the HsF diet (Zucker, -3.7%) or the HC diet (Wistar rats, -12.4%). This was not associated with lower weight gain relative to HC fed rats, because of increased food efficiencies in each line fed HsF and particularly LC-HsF-HP food. Zucker rats were less glucose tolerant than Wistar rats. Lowest glucose tolerances were found in HsF and particularly in LC-HsF-HP-fed animals irrespective of line, but this paralleled reduced plasma adiponectin levels, elevated plasma resistin levels, higher retroperitoneal fat masses, and reduced insulin sensitivity (indexed by insulin-induced hypoglycemia) only in Wistar rats. In Zucker rats, however, improved insulin responses during glucose tolerance testing and tendency toward increased insulin sensitivities were observed with HsF or LC-HsF-HP feeding relative to HC feeding. Thus, despite adverse consequences of LC-HsF diets on blood glucose homeostasis, principal differences exist in the underlying hormonal regulatory mechanisms, which could have benefits for B-cell functioning and insulin action in the obese state but not in the lean state.
Subject(s)
Dietary Carbohydrates/metabolism , Dietary Fats/metabolism , Dietary Proteins/metabolism , Energy Intake , Obesity/physiopathology , Thinness/physiopathology , Adaptation, Physiological , Animals , Body Weight , Eating , Energy Metabolism , Homeostasis , Insulin Resistance/physiology , Male , Rats , Rats, Wistar , Rats, ZuckerABSTRACT
Food restriction leads to a paradoxical increase in physical activity and further suppression of food intake, such as observed in anorexia nervosa.(1,2) To understand this pathophysiological process, we induced physical hyperactivity and self-starvation in rats by restricting food in the presence of running wheels. Normally, decreased melanocortin receptor activity will prevent starvation.(3,4) However, we found that self-starvation increased melanocortin receptors in the ventral medial hypothalamus, a brain region involved in eating behavior.(5) Suppression of melanocortin receptor activity, via central infusion of Agouti-related protein (AgRP), increased survival rate in these rats by counteracting physical hyperactivity, food intake suppression as well as deregulated body temperature. We conclude that self-starvation may result from insufficient suppression of central melanocortin receptor activity.
Subject(s)
Anorexia Nervosa/drug therapy , Anorexia Nervosa/metabolism , Motor Activity/drug effects , Proteins/metabolism , Proteins/pharmacology , Agouti-Related Protein , Animals , Animals, Outbred Strains , Caloric Restriction , Feeding Behavior/drug effects , Feeding Behavior/physiology , Female , Gene Expression/drug effects , Hypothalamo-Hypophyseal System/metabolism , Intercellular Signaling Peptides and Proteins , Pituitary-Adrenal System/metabolism , Pro-Opiomelanocortin/genetics , Rats , Rats, Wistar , Receptors, Corticotropin/metabolism , Receptors, Melanocortin , Ventromedial Hypothalamic Nucleus/metabolismABSTRACT
The CNS melanocortin (MC) system is implicated as a mediator of the central effects of leptin, and reduced activity of the CNS MC system promotes obesity in both rodents and humans. Because activation of CNS MC receptors has direct effects on autonomic outflow and metabolism, we hypothesized that food intake-independent mechanisms contribute to development of obesity induced by pharmacological blockade of MC receptors in the brain and that changes in hypothalamic neuropeptidergic systems known to regulate weight gain [i.e., corticotropin-releasing hormone (CRH), cocaine-amphetamine-related transcript (CART), proopiomelanocortin (POMC), and neuropeptide Y (NPY)] would trigger this effect. Relative to vehicle-treated controls, third intracerebroventricular (i3vt) administration of the MC receptor antagonist SHU9119 to rats for 11 d doubled food and water intake (toward the end of treatment) and increased body weight ( approximately 14%) and fat content ( approximately 90%), hepatic glycogen content ( approximately 40%), and plasma levels of cholesterol ( approximately 48%), insulin ( approximately 259%), glucagon ( approximately 80%), and leptin ( approximately 490%), whereas spontaneous locomotor activity and body temperature were reduced. Pair-feeding of i3vt SHU9119-treated animals to i3vt vehicle-treated controls normalized plasma levels of insulin, glucagon, and hepatic glycogen content, but only partially reversed the elevations of plasma cholesterol ( approximately 31%) and leptin ( approximately 104%) and body fat content ( approximately 27%). Reductions in body temperature and locomotor activity induced by i3vt SHU9119 were not reversed by pair feeding, but rather were more pronounced. None of the effects found can be explained by peripheral action of the compound. The obesity effects occurred despite a lack in neuropeptide expression responses in the neuroanatomical range selected across the arcuate (i.e., CART, POMC, and NPY) and paraventricular (i.e., CRH) hypothalamus. The results indicate that reduced activity of the CNS MC pathway promotes fat deposition via both food intake-dependent and -independent mechanisms.
Subject(s)
Behavior, Animal/physiology , Hypothalamus/metabolism , Obesity/metabolism , Receptors, Corticotropin/metabolism , Signal Transduction/physiology , Animals , Behavior, Animal/drug effects , Body Composition/drug effects , Body Temperature/drug effects , Cholesterol/blood , Drinking/drug effects , Eating/drug effects , Glucagon/blood , Hypothalamus/drug effects , Injections, Intraventricular , Insulin/blood , Leptin/blood , Male , Melanocyte-Stimulating Hormones/administration & dosage , Motor Activity/drug effects , Neurotransmitter Agents/genetics , Neurotransmitter Agents/metabolism , Pro-Opiomelanocortin/genetics , Pro-Opiomelanocortin/metabolism , RNA, Messenger/metabolism , Rats , Rats, Wistar , Receptors, Corticotropin/antagonists & inhibitors , Receptors, Melanocortin , Signal Transduction/drug effectsABSTRACT
We tested the hypothesis that excessive portal venous supply of long-chain fatty acids to the liver contributes to the development of insulin resistance via activation of the hypothalamus-pituitary-adrenal axis (HPA axis) and sympathetic system. Rats received an intraportal infusion of the long-chain fatty acid oleate (150 nmol/min, 24 h), the medium-chain fatty acid caprylate, or the solvent. Corticosterone (Cort) and norepinephrine (NE) were measured as indexes for HPA axis and sympathetic activity, respectively. Insulin sensitivity was assessed by means of an intravenous glucose tolerance test (IVGTT). Oleate infusion induced increases in plasma Cort (Delta = 13.5 +/- 3.6 microg/dl; P < 0.05) and NE (Delta = 235 +/- 76 ng/l; P < 0.05), whereas caprylate and solvent had no effect. The area under the insulin response curve to the IVGTT was larger in the oleate-treated group than in the caprylate and solvent groups (area = 220 +/- 35 vs. 112 +/- 13 and 106 +/- 8, respectively, P < 0.05). The area under the glucose response curves was comparable [area = 121 +/- 13 (oleate) vs. 135 +/- 20 (caprylate) and 96 +/- 11 (solvent)]. The results are consistent with the concept that increased portal free fatty acid is involved in the induction of visceral obesity-related insulin resistance via activation of the HPA axis and sympathetic system.
Subject(s)
Hypothalamo-Hypophyseal System/metabolism , Microvascular Angina/metabolism , Oleic Acid/blood , Pituitary-Adrenal System/metabolism , Sympathetic Nervous System/metabolism , Animals , Blood Pressure/physiology , Caprylates/blood , Caprylates/pharmacokinetics , Corticosterone/blood , Epinephrine/blood , Glucose Tolerance Test , Heart Rate/physiology , Hypothalamo-Hypophyseal System/drug effects , Insulin Resistance/physiology , Liver/blood supply , Liver/metabolism , Male , Microvascular Angina/chemically induced , Norepinephrine/blood , Obesity/chemically induced , Obesity/metabolism , Oleic Acid/pharmacokinetics , Pituitary-Adrenal System/drug effects , Portal Vein/physiology , Rats , Rats, Wistar , Sympathetic Nervous System/drug effects , Triglycerides/metabolismABSTRACT
Many studies have indicated that neuropeptide Y (NPY) stimulates and leptin inhibits food intake. In line with this, intracerebroventricular injection of NPY (10 microg) stimulated and leptin (10 microg) inhibited intake of a sucrose solution when female rats were required to obtain the solution from a bottle. However, NPY inhibited and leptin stimulated intake if the solution was infused intraorally. Thus NPY stimulates the responses used to obtain food but inhibits those used to consume food, and leptin has the opposite effects. To test the specificity of these responses the sexual behavior of male rats was examined. NPY-treated males showed minor deficits in sexual behavior but chose to ingest a sucrose solution rather than copulate with a female if offered the choice. By contrast, leptin-treated males ingested little sucrose and displayed an increase in ejaculatory frequency if given the same choice. It is suggested that NPY is not merely an orexigenic peptide, but one that directs attention toward food. Similarly, leptin may not be an anorexic peptide, but one that diverts attention away from food toward alternate stimuli.
Subject(s)
Appetite/drug effects , Feeding Behavior/drug effects , Leptin/pharmacology , Neuropeptide Y/pharmacology , Sexual Behavior, Animal/drug effects , Administration, Oral , Animals , Arcuate Nucleus of Hypothalamus/physiology , Dietary Sucrose/pharmacology , Female , Injections, Intraventricular , Male , Rats , Rats, WistarABSTRACT
Energy balance is the resultant of ingested calories and energy expenditure and is generally maintained within narrow limits over prolonged periods. Exercise leads to an increase in energy expenditure which is, in the long-term, counteracted by increased energy intake. Evidence for this comes from a study in voluntarily running female rats that increased their daily food intake to 130% of the sedentary controls. In contrast, when considered on a short-term basis, exercise will suppress food intake to prevent a potentially dangerous disruption of energy substrate homeostasis. Studies in permanently cannulated rats submitted to a test meal and 2 hrs swimming reveal that both food intake and exercise lead to increases in glucose and free fatty acid (FFA) levels in the blood. These changes in glucose and FFA, combined with the exercise-induced alteration in among others glucagon, corticotropin releasing hormone (CRH) and body temperature, may lead to the short-term anorexic effect of exercise.
Subject(s)
Energy Intake , Energy Metabolism , Exercise/physiology , Animals , Eating , Humans , Physical Conditioning, Animal/physiologySubject(s)
Fatty Acids, Nonesterified/blood , Hypothalamo-Hypophyseal System/metabolism , Insulin Resistance , Liver/metabolism , Pituitary-Adrenal System/metabolism , Portal Vein , Sympathetic Nervous System/physiopathology , Animals , Diabetes Mellitus, Type 2/metabolism , Humans , Hypertension/metabolism , Sympathetic Nervous System/metabolismABSTRACT
We asked whether the likelihood for mice of the C57BL/6J strain to develop glucose intolerance when fed a high-fat diet is related to the increase in circulating levels of leptin or free fatty acids (FFA). We therefore administered a high-fat diet (58% fat) or a control diet (11% fat) for 1.5 years. NMRI mice were used as a more glucose-tolerant control group. After a high-fat diet, the area under the glucose curve following an intraperitoneal glucose challenge (1 g/kg) increased more markedly in C57BL/6J mice (by 42+/-8%) than in NMRI mice (by 21+/-3%, P = 0.007). Plasma levels of insulin, leptin and FFA increased in both strains of mice, whereas plasma glucose levels were elevated after the high-fat diet only in C57BL/6J mice. The slope of the relationship between body weight and plasma leptin was higher in C57BL/6J mice than in NMRI mice. suggesting leptin insensitivity. Circulating leptin correlated to circulating insulin in both strains of mice, whereas plasma FFA correlated to plasma insulin in NMRI mice but not in C57BL/6J mice. These correlations remained significant after adjustment for body weight. The results show that elevated leptin and FFA levels evolve after high-fat feeding in mice, in conjunction with evolvement of glucose intolerance and hyperglycemia.
Subject(s)
Dietary Fats/administration & dosage , Glucose Intolerance , Proteins/metabolism , Animals , Body Weight , Fatty Acids, Nonesterified/blood , Hyperglycemia/blood , Insulin/blood , Insulin Resistance , Leptin , Mice , Mice, Inbred C57BLABSTRACT
Although pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates insulin secretion, its net influence on glucose homeostasis in vivo has not been established. We therefore examined the action of PACAP-27 and PACAP-38 on insulin secretion, insulin sensitivity, and glucose disposal as derived from the minimal model of glucose disappearance during an intravenous glucose tolerance test in anesthetized mice. PACAP-27 and PACAP-38 markedly and equipotently potentiated glucose-stimulated insulin secretion, with a half-maximal effect at 33 pmol/kg. After PACAP-27 or PACAP-38 (1.3 nmol/kg), the acute (1-5 min) insulin response was 3.8 +/- 0.4 nmol/l (PACAP-27) and 3.3 +/- 0.3 nmol/l (PACAP-38), respectively, vs. 1.4 +/- 0.1 nmol/l after glucose alone (P < 0.001), and the total area under the curve for insulin (AUCinsulin) was potentiated by 60% (P < 0.001). In contrast, PACAP-27 and PACAP-38 reduced the insulin sensitivity index (SI) [0.23 +/- 0.04 10(-4) min-1/(pmol/l) for PACAP-27 and 0.29 +/- 0.06 10(-4) min-1/(pmol/l) for PACAP-38 vs. 0.46 +/- 0.02 10(-4) min-1/(pmol/l) for controls (P < 0.01)]. Furthermore, PACAP-27 or PACAP-38 did not affect glucose elimination determined as glucose half-time or the glucose elimination rate after glucose injection or the area under the curve for glucose. Moreover, glucose effectiveness and the global disposition index (AUCinsulin times SI) were not affected by PACAP-27 or PACAP-38. Finally, when given together with glucose, PACAP-27 did not alter plasma glucagon or norepinephrine levels but significantly increased plasma epinephrine levels. We conclude that PACAP, besides its marked stimulation of insulin secretion, also inhibits insulin sensitivity in mice, the latter possibly explained by increased epinephrine. This complex action explains why the peptide does not enhance glucose disposal.
Subject(s)
Insulin Resistance/physiology , Insulin/metabolism , Neuropeptides/pharmacology , Neurotransmitter Agents/pharmacology , Animals , Drug Combinations , Epinephrine/blood , Glucagon/blood , Glucose/pharmacokinetics , Glucose/pharmacology , Injections, Intravenous , Insulin/blood , Insulin/pharmacology , Insulin Secretion , Mice , Mice, Inbred Strains , Pituitary Adenylate Cyclase-Activating Polypeptide , Sodium Chloride/pharmacologyABSTRACT
Leptin, a hormone secreted by adipose tissue in proportion to body adiposity, is proposed to be involved in the central nervous regulation of food intake and body weight. In addition, evidence is emerging that leptin regulates neuroendocrine and metabolic functions as well, presumably via its action in the central nervous system (CNS). To investigate this regulatory effect of leptin, we infused 3.5 microg of human leptin directly into the third cerebral ventricle (i3vt) of lean male Long-Evans rats, 90 min before the onset of their dark phase. Before and after infusion, blood samples were withdrawn through indwelling catheters for assessment of hormonal (plasma corticosterone, insulin, leptin), autonomic (plasma norepinephrine, epinephrine), and metabolic (plasma glucose) parameters. I3vt leptin caused an increase in plasma corticosterone and plasma leptin levels relative to the control condition. The effects of i3vt leptin on corticosterone secretion became particularly apparent after the onset of the dark phase. The results of the present study indicate that i3vt leptin stimulates the hypothalamo-pituitary-adrenal (HPA) axis, particularly when rats normally encounter their largest meals. These results are consistent with the possibility that high circulating leptin levels may underlie the increased activity of the HPA axis that is generally characteristic of human obesity and most animal models of obesity.
Subject(s)
Cerebral Ventricles/physiology , Circadian Rhythm/physiology , Corticosterone/metabolism , Proteins/pharmacology , Analysis of Variance , Animals , Blood Glucose/drug effects , Blood Glucose/metabolism , Cerebral Ventricles/drug effects , Corticosterone/blood , Darkness , Epinephrine/blood , Humans , Hypothalamo-Hypophyseal System/physiology , Infusions, Parenteral , Insulin/blood , Insulin/metabolism , Insulin Secretion , Leptin , Light , Male , Norepinephrine/blood , Obesity , Pituitary-Adrenal System/physiology , Proteins/administration & dosage , Proteins/pharmacokinetics , RatsABSTRACT
To study islet function following reduced insulin sensitivity, we examined mice of the C57BL/6J strain, the genotype of which carries an increased propensity to develop insulin resistance when metabolically challenged. The mice received either a high-fat diet (58% fat on an energy basis) or a control diet (11% fat) for 12 weeks. The body weight of mice on the high-fat diet increased significantly more than that of mice on the control diet (25.8 +/- 0.4 v 21.3 +/- 0.2 g, P < .001). Already after 1 week on the high-fat diet, a significant hyperglycemia accompanied by hyperinsulinemia had evolved, indicative of insulin resistance. After 12 weeks, plasma glucose levels for high-fat diet-treated mice were 7.5 +/- 0.1 mmol/L, versus 6.5 +/- 0.1 mmol/L in controls (P < .001); corresponding values for plasma insulin were 248 +/- 17 and 104 +/- 7 pmol/L, respectively (P < .001). Mice given a high-fat diet also had elevated levels of total cholesterol, triglycerides, and free fatty acids (FFAs) compared with controls. After 4, 8, and 12 weeks, glucose (2.8, 8.3, or 16.7 mmol/kg) or the cholinergic agonist carbachol (0.16 or 0.53 micromol/kg) was injected intraperitoneally. The insulinotropic response to glucose was not different between the two groups after 4 or 8 weeks, whereas after 12 weeks, glucose-induced insulin secretion was markedly impaired in high-fat diet-treated mice (P < .001). In contrast, after 8 and 12 weeks on a high-fat diet, carbachol-stimulated insulin secretion was potentiated (P < .01), whereas carbachol-stimulated glucagon secretion was not significantly altered. Furthermore, after 12 weeks on the high-fat diet, insulin secretion from isolated islets was impaired at glucose levels of 8.3, 11.1, and 16.7 mmol/L (P < or = .05). Moreover, islet morphology as examined by immunocytochemistry using insulin antibodies and islet innervation, as revealed by immunostaining of tyrosine hydroxylase (TH), neuropeptide Y (NPY), galanin, vasoactive intestinal polypeptide (VIP), and substance P (SP) were unaffected by the high-fat diet for 12 weeks. However, quantitative in situ hybridization showed a 3.5-fold upregulation of insulin gene expression in response to the high-fat diet (P < .001) despite unaltered B-cell mass and pancreatic insulin content. We conclude that as little as 1 week of treatment with a high-fat diet induces insulin resistance in C57BL/6J mice. This is accompanied later by hyperlipemia, potentiated carbachol-stimulated insulin secretion, and increased insulin gene expression but impaired glucose-stimulated insulin secretion. We suggest that after several weeks' duration, insulin resistance is accompanied by enhanced islet sensitivity to cholinergic activation and exaggerated insulin gene expression, whereas the failing islet sensitivity to glucose represents decompensation.
Subject(s)
Carbachol/pharmacology , Dietary Fats/pharmacology , Glucose/pharmacology , Insulin Resistance/physiology , Muscarinic Agonists/pharmacology , Animals , Blood Glucose/analysis , Body Weight/physiology , Cholesterol/blood , Eating/physiology , Fatty Acids, Nonesterified/blood , Female , Fluorescent Antibody Technique, Indirect , Galanin/analysis , Galanin/metabolism , Gene Expression , Genotype , Glucagon/blood , Glucose/analysis , Immunohistochemistry , In Situ Hybridization , Insulin/blood , Insulin/genetics , Insulin/metabolism , Islets of Langerhans/chemistry , Islets of Langerhans/metabolism , Islets of Langerhans/physiology , Mice , Mice, Inbred C57BL , Neuropeptide Y/analysis , Neuropeptide Y/metabolism , Substance P/analysis , Substance P/metabolism , Triglycerides/blood , Vasoactive Intestinal Peptide/analysis , Vasoactive Intestinal Peptide/metabolismABSTRACT
The autonomic nervous system plays an important role in the regulation of body processes in health and disease. Overfeeding and obesity (a disproportional increase of the fat mass of the body) are often accompanied by alterations in both sympathetic and parasympathetic autonomic functions. The overfeeding-induced changes in autonomic outflow occur with typical symptoms such as adiposity and hyperinsulinemia. There might be a causal relationship between autonomic disturbances and the consequences of overfeeding and obesity. Therefore studies were designed to investigate autonomic functioning in experimentally and genetically hyperphagic rats. Special emphasis was given to the processes that are involved in the regulation of peripheral energy substrate homeostasis. The data revealed that overfeeding is accompanied by increased parasympathetic outflow. Typical indices of vagal activity (such as the cephalic insulin release during food ingestion) were increased in all our rat models for hyperphagia. Overfeeding was also accompanied by increased sympathetic tone, reflected by enhanced baseline plasma norepinephrine (NE) levels in both VMH-lesioned animals and rats rendered obese by hyperalimentation. Plasma levels of NE during exercise were, however, reduced in these two groups of animals. This diminished increase in the exercise-induced NE outflow could be normalized by prior food deprivation. It was concluded from these experiments that overfeeding is associated with increased parasympathetic and sympathetic tone. In models for hyperphagia that display a continuously elevated nutrient intake such as the VMH-lesioned and the overfed rat, this increased sympathetic tone was accompanied by a diminished NE response to exercise. This attenuated outflow of NE was directly related to the size of the fat reserves, indicating that the feedback mechanism from the periphery to the central nervous system is altered in the overfed state.
Subject(s)
Autonomic Nervous System/physiopathology , Hyperphagia/physiopathology , Obesity/metabolism , Obesity/physiopathology , Animals , Disease Models, Animal , Energy Metabolism , Homeostasis , Humans , Hyperphagia/metabolism , RatsABSTRACT
Obesity is often accompanied by alterations in both sympathetic and parasympathetic autonomic functions. The present paper summarizes the results of a number of studies designed to investigate autonomic functioning in normal, genetically, and experimentally obese rats. Particular emphasis is given to autonomic functioning and dysfunctioning in relation to the processes that are involved in the regulation of peripheral energy substrate homeostasis. It is concluded that alterations in autonomic regulation in obesity are determined by causal factors such as overeating, genetic make-up, age and/or the duration of obesity.