ABSTRACT
Digitization of phase-modulated carrier signals with a commercially available analog-to-digital converter (ADC) is a common task in many communication and sensor applications. ADCs deliver phase-modulated digital carrier signals, which are numerically demodulated in order to extract the relevant information. However, the limited dynamic ranges of available ADCs limit the carrier-to-noise ratio of carrier signals after digitization. Correspondingly, the resolution of the demodulated digital signal is degraded. We demonstrate a sampling method with a simple demodulation scheme for phase-modulated signals with a small modulation index. Our new scheme overcomes the limitation due to digital noise defined by the ADC. Through simulations and experiments, we provide evidence that our method can improve the resolution of the demodulated digital signal significantly, when the carrier-to-noise ratio of phase-modulated signals is limited by digital noise. We employ our sampling and demodulation scheme to solve the problem of a possible degradation of measurement resolution after digital demodulation in heterodyne interferometers measuring small vibration amplitudes.
ABSTRACT
To elucidate the role of phospholipid asymmetry in calcium-phosphate-induced fusion of human erythrocytes, we examined the interaction of erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion of human erythrocytes was monitored by light microscopy as well as spectrophotometrically by the octadecylrhodamine dequenching assay. Phospholipid translocation and distribution between the inner and the outer leaflet of intact red blood cells were determined with spin-labeled phosphatidylserine (PS), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). Significant fusion of lipid-asymmetric red blood cells where PS and PE are predominantly oriented to the inner leaflet was only observed at Ca2+ concentrations greater than or equal to 10 mM (in the presence of 10 mM phosphate buffer) while fusion of lipid-symmetric erythrocyte membranes was established at greater than or equal to 1.5 mM Ca2+. The Ca2+ threshold of fusion of lipid-asymmetric red blood cells was significantly reduced (i) after exposure of PS to the outer layer but not after redistribution of PE alone, and (ii) upon incorporation of spin-labeled PS into the outer leaflet of red blood cells. Spin-labeled PE or PC did not affect fusion, suggesting that the serine headgroup is an important factor in calcium-phosphate-induced fusion.
